Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2875 and PP_2876 are separated by 282 nucleotidesPP_2876 and PP_2877 are separated by 106 nucleotidesPP_2877 and PP_2878 are separated by 22 nucleotidesPP_2878 and PP_2879 are separated by 52 nucleotides PP_2875: PP_2875 - conserved protein of unknown function, at 3,276,345 to 3,276,695 _2875 PP_2876: PP_2876 - Beta-lactamase, at 3,276,978 to 3,278,120 _2876 PP_2877: PP_2877 - putative osmotic pressure-regulated transporter, at 3,278,227 to 3,279,228 _2877 PP_2878: PP_2878 - conserved protein of unknown function, at 3,279,251 to 3,279,433 _2878 PP_2879: PP_2879 - conserved exported protein of unknown function, at 3,279,486 to 3,280,079 _2879
Group Condition PP_2875 PP_2876 PP_2877 PP_2878 PP_2879
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -0.1 +0.1 -3.8 +0.2
carbon source L-Leucine (C) N.D. +0.3 +0.1 -3.6 +0.1
pH Growth at pH6 and (C) D-Glucose N.D. -0.2 -0.3 -2.5 +0.2
phage P. putida PUT 7 MOI 1 N.D. -0.6 -0.4 -1.0 -0.5
temperature Growth at 30C N.D. -0.1 -0.3 -2.4 +0.4
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. +0.2 -0.4 -2.0 -0.2
carbon source ferulate (C); with MOPS N.D. -0.3 -0.4 -1.4 -0.1
stress Growth with Chloride 500 mM N.D. -0.4 +0.1 -1.7 -0.1
carbon source D-Glucose (C); with MOPS N.D. -0.3 +0.2 -2.1 +0.1
phage P. putida Sci Big MOI 0.1 N.D. -0.2 -0.4 -1.7 +0.4
temperature shift Temperature shift 10_to_30; with MOPS N.D. -0.1 -0.2 -1.6 +0.0
carbon source D-Glucose (C) N.D. +0.2 -0.0 -2.2 +0.3
temperature shift Temperature shift 30_to_34; with MOPS N.D. -0.1 +0.2 -2.0 +0.2
nitrogen source no extra (N); with MOPS N.D. -0.3 +0.0 -1.5 +0.1
phage P. putida Sci small MOI 0.1 N.D. +0.2 -1.4 -0.4 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 -0.3 -1.2 -0.2
temperature Growth at 10C; with MOPS N.D. -0.1 -0.1 -1.4 +0.3
nitrogen source Ammonium chloride (N) N.D. -0.2 +0.1 -1.4 +0.3
pH Growth at pH7 and (C) Trisodium citrate N.D. -0.2 +0.3 -1.8 +0.5
carbon source Trisodium citrate (C) N.D. +0.0 +0.4 -1.5 +0.3
carbon source Tween 20 (C) N.D. -0.4 +0.3 -0.9 +0.3
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.3 +0.5 -1.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs N.D. +0.1 +0.5 -1.5 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.1 -0.5 -0.6 +1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.2 -0.6 +1.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.3 +0.1 +1.2 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.3 -0.3 +1.0 +0.6
phage PUT8 MOI 0.1 N.D. +0.1 -0.0 +1.8 -0.3
carbon source L-Glutamic (C) N.D. +1.3 +0.0 +0.7 +0.4
pH Valerolactone (C) pH 6.5 N.D. +0.1 +0.2 +2.2 +0.2
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