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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2816 and PP_2817 are separated by 91 nucleotides
PP_2817 and PP_2818 are separated by 15 nucleotides
PP_2818 and PP_2819 overlap by 4 nucleotides
PP_2819 and PP_2820 are separated by 82 nucleotides
PP_2816: PP_2816 - putative Transcriptional regulator NfxB, at 3,212,276 to 3,212,842
_2816
PP_2817: PP_2817 - Multidrug efflux RND membrane fusion protein MexC, at 3,212,934 to 3,214,070
_2817
PP_2818: PP_2818 - Multidrug efflux RND transporter MexD, at 3,214,086 to 3,217,214
_2818
PP_2819: PP_2819 - Outer membrane protein OprJ, at 3,217,211 to 3,218,617
_2819
PP_2820: PP_2820 - HTH-type transcriptional regulator nfxB, at 3,218,700 to 3,219,257
_2820
Group
Condition
PP
_2816
PP
_2817
PP
_2818
PP
_2819
PP
_2820
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.2
-0.5
-0.2
-0.0
-5.5
nitrogen source
D-2-Aminobutyric (N)
-0.9
-0.1
-0.1
-0.6
-3.5
nitrogen source
D-2-Aminobutyric (N)
+0.1
-0.4
-0.3
-0.6
-3.2
carbon source
Vanillin (C)
-0.9
-0.1
+0.0
-0.4
-3.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.5
+0.0
+0.1
-0.1
-3.7
carbon source
Delta-Undecalactone (C)
+1.0
-2.5
-2.3
-0.2
-0.1
carbon source
Delta-Undecalactone (C)
+0.1
-1.6
-1.7
-0.5
-0.3
carbon source
3-methyl-1-butanol (C)
-1.0
+0.1
-0.1
-0.1
-2.8
temperature shift
Temperature shift 10_to_25; with MOPS
-0.7
+0.1
-0.2
+0.1
-2.7
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM
-0.3
-0.3
-0.1
-0.9
-1.9
carbon source
Vanillin (C)
-0.8
-0.1
+0.0
+0.0
-2.7
nitrogen source
NAG (N); with MOPS
-1.8
-0.1
-0.3
+0.6
-1.8
carbon source
ferulate (C); with MOPS
-1.0
-0.2
-0.0
-0.4
-1.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.9
-0.3
+0.0
+0.7
-2.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.2
-0.0
+0.2
+0.1
-3.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.8
+0.6
-0.2
+0.3
-2.7
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.4
-0.2
-0.2
-0.7
-2.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.5
+0.8
-0.4
-2.7
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
+0.5
-0.7
-0.2
+0.0
-2.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
+0.3
+0.1
+0.1
+0.3
-3.1
nitrogen source
Hippuric (N)
+0.5
-0.1
-0.0
-0.2
-2.4
nitrogen source
Hippuric (N)
+0.2
-0.2
-0.1
+0.5
-2.4
carbon source
Tween 20 (C)
+0.1
+0.3
+0.2
-0.7
-2.1
carbon source
acetate (C)
+0.6
-0.4
-0.1
-0.7
-1.4
pH
pH 6
+0.5
+0.1
-0.0
+0.1
-2.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.6
+0.5
+0.2
-1.2
-0.8
carbon source
1,3-Hexanediol (C)
+0.7
+0.0
-0.1
+0.3
-2.7
phage
P. putida Sci small MOI 0.1
-0.1
-0.2
-0.4
-1.6
+1.3
carbon source
L-Lysine (C)
+0.8
+0.2
-0.1
+0.4
-2.2
carbon source
Delta-Decalactone (C)
+1.3
-0.7
-0.6
-0.1
+0.7
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