Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2816 and PP_2817 are separated by 91 nucleotidesPP_2817 and PP_2818 are separated by 15 nucleotidesPP_2818 and PP_2819 overlap by 4 nucleotidesPP_2819 and PP_2820 are separated by 82 nucleotides PP_2816: PP_2816 - putative Transcriptional regulator NfxB, at 3,212,276 to 3,212,842 _2816 PP_2817: PP_2817 - Multidrug efflux RND membrane fusion protein MexC, at 3,212,934 to 3,214,070 _2817 PP_2818: PP_2818 - Multidrug efflux RND transporter MexD, at 3,214,086 to 3,217,214 _2818 PP_2819: PP_2819 - Outer membrane protein OprJ, at 3,217,211 to 3,218,617 _2819 PP_2820: PP_2820 - HTH-type transcriptional regulator nfxB, at 3,218,700 to 3,219,257 _2820
Group Condition PP_2816 PP_2817 PP_2818 PP_2819 PP_2820
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.2 -0.5 -0.2 -0.0 -5.5
nitrogen source D-2-Aminobutyric (N) -0.9 -0.1 -0.1 -0.6 -3.5
nitrogen source D-2-Aminobutyric (N) +0.1 -0.4 -0.3 -0.6 -3.2
carbon source Vanillin (C) -0.9 -0.1 +0.0 -0.4 -3.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 +0.0 +0.1 -0.1 -3.7
carbon source Delta-Undecalactone (C) +1.0 -2.5 -2.3 -0.2 -0.1
carbon source Delta-Undecalactone (C) +0.1 -1.6 -1.7 -0.5 -0.3
carbon source 3-methyl-1-butanol (C) -1.0 +0.1 -0.1 -0.1 -2.8
temperature shift Temperature shift 10_to_25; with MOPS -0.7 +0.1 -0.2 +0.1 -2.7
mixed carbon source mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM -0.3 -0.3 -0.1 -0.9 -1.9
carbon source Vanillin (C) -0.8 -0.1 +0.0 +0.0 -2.7
nitrogen source NAG (N); with MOPS -1.8 -0.1 -0.3 +0.6 -1.8
carbon source ferulate (C); with MOPS -1.0 -0.2 -0.0 -0.4 -1.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 -0.3 +0.0 +0.7 -2.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 -0.0 +0.2 +0.1 -3.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.8 +0.6 -0.2 +0.3 -2.7
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.4 -0.2 -0.2 -0.7 -2.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.5 +0.8 -0.4 -2.7
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.5 -0.7 -0.2 +0.0 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +0.3 +0.1 +0.1 +0.3 -3.1
nitrogen source Hippuric (N) +0.5 -0.1 -0.0 -0.2 -2.4
nitrogen source Hippuric (N) +0.2 -0.2 -0.1 +0.5 -2.4
carbon source Tween 20 (C) +0.1 +0.3 +0.2 -0.7 -2.1
carbon source acetate (C) +0.6 -0.4 -0.1 -0.7 -1.4
pH pH 6 +0.5 +0.1 -0.0 +0.1 -2.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.6 +0.5 +0.2 -1.2 -0.8
carbon source 1,3-Hexanediol (C) +0.7 +0.0 -0.1 +0.3 -2.7
phage P. putida Sci small MOI 0.1 -0.1 -0.2 -0.4 -1.6 +1.3
carbon source L-Lysine (C) +0.8 +0.2 -0.1 +0.4 -2.2
carbon source Delta-Decalactone (C) +1.3 -0.7 -0.6 -0.1 +0.7
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