Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2647 and PP_2648 are separated by 292 nucleotidesPP_2648 and PP_2649 are separated by 234 nucleotidesPP_2649 and PP_2650 are separated by 142 nucleotidesPP_2650 and PP_2651 are separated by 44 nucleotides PP_2647: PP_2647 - Major facilitator family transporter, at 3,033,962 to 3,035,188 _2647 PP_2648: PP_2648 - Universal stress protein family, at 3,035,481 to 3,036,410 _2648 PP_2649: PP_2649 - Transcriptional regulator, LysR family, at 3,036,645 to 3,037,586 _2649 PP_2650: PP_2650 - putative 4-hydroxybutyrate dehydrogenase, at 3,037,729 to 3,038,871 _2650 PP_2651: PP_2651 - Major facilitator family transporter, at 3,038,916 to 3,040,259 _2651
Group Condition PP_2647 PP_2648 PP_2649 PP_2650 PP_2651
carbon source carbon source 3-O-methyl-glucose 10 mM +0.2 -0.6 -0.6 -1.6 -0.2
carbon source Myristic (C) -0.8 -1.0 -0.0 -0.9 -0.0
stress R2A with Fusaric 240 ug/mL -0.1 -0.1 -0.1 -1.8 -0.4
carbon source carbon source 4-vinylphenol 0.01 mM +0.2 -0.1 -1.1 -1.2 -0.2
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.4 -0.6 -0.4 -1.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 +0.6 +0.3 -1.1 -0.9
pH pH 7 +0.5 -1.0 +0.0 -0.8 -0.4
nitrogen source D-2-Aminobutyric (N) -0.2 -0.7 +0.3 -1.2 +0.3
temperature shift Temperature shift 30_to_21; with MOPS -0.2 +0.6 -0.2 -1.7 +0.0
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.5 -0.4 -0.6 -0.5 -0.3
pH Growth at pH7 and (C) Trisodium citrate +0.2 -0.1 -1.4 +0.6 -0.6
nitrogen source Urea (N); with MOPS +0.3 -1.1 +0.1 -0.6 +0.1
phage P. putida PUT 7 MOI 1 +0.2 +0.5 +0.3 -1.5 -0.0
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM +0.1 +0.6 -0.1 -1.3 +0.2
temperature shift Temperature shift 10_to_25; with MOPS +0.4 +0.3 -1.1 +0.3 -0.3
pH pH 5 +0.3 -0.3 +0.6 -0.6 -0.5
pH Growth at pH7 and (C) D-Glucose +0.3 +0.2 +0.6 -0.9 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 +0.4 -0.9 +0.3 +0.2
carbon source D-Glucose (C); with MOPS -0.3 +0.6 -1.0 +0.5 -0.0
nitrogen source nitrogen source nitrate 20mM -0.2 -1.0 +0.3 +0.9 +0.1
phage P. putida Sci small MOI 0.1 +0.3 -0.9 +1.1 +0.5 -0.7
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.8 +0.3 +1.2 -0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.4 +1.1 -0.6 -0.4
nitrogen source NAG (N); with MOPS +0.4 +0.6 -1.4 +1.3 -0.3
carbon source Valerolactone (C) -0.2 +0.5 +1.1 -0.0 -0.5
phage P. putida Missley MOI 0.1 -0.4 +1.0 -0.3 +0.0 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 -0.2 +1.6 -0.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 +0.7 +0.7 -0.3 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.1 -0.4 +1.1 +0.5
temperature shift Temperature shift 30_to_25; with MOPS +0.2 +0.6 +0.6 +0.5 +0.4
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