Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2595 and PP_2596 overlap by 7 nucleotidesPP_2596 and PP_2597 are separated by 109 nucleotidesPP_2597 and PP_2598 are separated by 205 nucleotidesPP_2598 and PP_2599 overlap by 26 nucleotides PP_2595: PP_2595 - putative ABC transporter, permease/ATP-binding protein, at 2,964,869 to 2,966,626 _2595 PP_2596: PP_2596 - putative ABC transporter, permease/ATP-binding protein, at 2,966,620 to 2,968,413 _2596 PP_2597: PP_2597 - conserved protein of unknown function, at 2,968,523 to 2,969,134 _2597 PP_2598: PP_2598 - conserved protein of unknown function, at 2,969,340 to 2,969,687 _2598 PP_2599: PP_2599 - Chemotaxis sensory transducer family protein, at 2,969,662 to 2,972,052 _2599
Group Condition PP_2595 PP_2596 PP_2597 PP_2598 PP_2599
phage P. putida Sci small MOI 0.1 -1.1 -0.2 -1.2 N.D. -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.1 -2.0 N.D. +0.2
carbon source Myristic (C) -0.8 -0.4 -1.1 N.D. -0.0
nitrogen source D-2-Aminobutyric (N) -0.5 -0.9 -0.8 N.D. -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.8 -0.3 -0.5 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 -1.0 -0.9 N.D. -0.2
nitrogen source nitrogen source nitrite 0.5mM -0.5 -0.7 -0.4 N.D. -0.0
stress MOPS minimal media_Glucose20mM with Chloride 200 mM -0.6 -0.3 -0.9 N.D. +0.1
phage P. putida Sci small MOI 0.1 -0.1 -0.6 -0.3 N.D. -0.7
temperature shift Temperature shift 10_to_25; with MOPS -1.0 +0.2 -0.2 N.D. -0.3
phage P. putida PUT 7 MOI 1 -0.3 -0.4 +0.2 N.D. -0.8
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 -0.3 -1.3 N.D. +0.3
nitrogen source Propandiamine (N) -0.9 -0.4 +0.4 N.D. -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.3 -0.7 N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 -0.5 -0.3 N.D. -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.4 -0.5 -0.8 N.D. +0.1
phage P. putida Sci Big MOI 0.1 +0.7 -0.5 -0.8 N.D. -0.2
stress R2A with Fusaric 480 ug/mL +0.4 +0.1 -0.6 N.D. -0.4
temperature shift Temperature shift 30_to_21; with MOPS +0.5 -0.2 -0.9 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.4 -0.6 N.D. +0.2
pH Growth at pH7 and (C) D-Glucose +0.3 +0.4 -1.4 N.D. +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.6 -0.3 +0.5 N.D. +0.1
nitrogen source 2,6-diaminopimelic (N) +0.6 -0.6 +0.2 N.D. -0.3
pH Growth at pH7 and (C) Trisodium citrate +0.1 +0.4 -0.8 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 +0.5 -0.6 N.D. -0.1
pH Growth at pH6 and (C) D-Glucose +0.3 +0.1 +0.5 N.D. -0.6
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.4 -0.4 +0.8 N.D. -0.2
nitrogen source Ammonium chloride (N) -0.5 +0.4 +0.5 N.D. +0.3
stress 1-Pentanol 0.3 vol% +0.8 -0.1 +0.5 N.D. -0.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.8 -0.2 +0.4 N.D. +0.1
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