Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2547 and PP_2548 are separated by 243 nucleotidesPP_2548 and PP_2549 are separated by 14 nucleotidesPP_2549 and PP_2550 are separated by 97 nucleotidesPP_2550 and PP_2551 are separated by 137 nucleotides PP_2547: PP_2547 - Transcriptional regulator, LysR family, at 2,891,898 to 2,892,767 _2547 PP_2548: PP_2548 - putative Sugar MFS transporter, at 2,893,011 to 2,894,327 _2548 PP_2549: PP_2549 - conserved protein of unknown function, at 2,894,342 to 2,895,733 _2549 PP_2550: PP_2550 - putative transcriptional regulatory protein, at 2,895,831 to 2,896,544 _2550 PP_2551: PP_2551 - Transcriptional regulator, LysR family, at 2,896,682 to 2,897,626 _2551
Group Condition PP_2547 PP_2548 PP_2549 PP_2550 PP_2551
mixed carbon source mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM +0.2 +0.1 +0.1 -3.1 -2.9
mixed carbon source mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM -0.4 +0.1 +0.4 -2.4 -2.9
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.9 -0.3 -0.4 -1.7 -0.4
carbon source ferulate (C); with MOPS -0.0 -0.0 -0.5 -1.9 -0.3
stress R2A with Fusaric 240 ug/mL -0.5 -0.2 -0.6 -1.5 +0.1
pH Growth at pH6 and (C) D-Glucose -2.7 +0.3 +0.4 -0.4 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.1 -0.1 -0.0 -0.6 -0.5
phage P. putida PUT 7 MOI 1 -1.5 +0.2 -0.2 -0.8 -0.0
micoeukaryotes Acanthamoeba sp., muxed culture-2 -0.2 -0.4 -0.2 -1.4 -0.1
stress Growth with Chloride 200 mM +0.3 -0.4 +0.1 -1.6 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 -0.6 -0.1 -1.1 +0.4
carbon source carbon source D-Glucose 55.5 mM +0.0 -0.0 +0.8 -2.9 +0.4
phage P. putida Sci small MOI 0.1 +0.1 +0.5 -0.8 -0.5 -1.0
nitrogen source nitrate (N) -1.1 -0.3 -0.1 +0.4 -0.6
temperature shift Temperature shift 30_to_25; with MOPS -0.7 -0.1 -0.2 -1.0 +0.5
nitrogen source D-2-Aminobutyric (N) +0.7 -0.3 -0.1 -1.4 -0.1
nitrogen source D-2-Aminobutyric (N) -1.0 -0.7 +0.4 +0.5 -0.2
carbon source Valeric (C) +0.8 +0.0 +0.2 -0.4 -1.0
phage P. putida Sci small MOI 0.1 -1.1 +0.4 +0.7 -0.1 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 +0.3 -0.4 +0.8 -0.9
carbon source carbon source Bisabolene 48.9 mM +0.6 -0.2 +0.4 -1.2 +0.4
temperature Growth at 25C -0.9 +0.2 +0.3 +0.4 +0.7
carbon source 4-Aminobutyric 10 mM (C) +0.3 +0.3 +0.5 +1.0 -0.6
pH pH 8 +1.1 +0.0 +0.7 +0.2 +0.4
carbon source Tween 20 (C) +0.3 +0.4 +0.5 +0.9 +0.4
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.4 -0.2 +1.0 +0.3 +1.2
carbon source carbon source 3-O-methyl-glucose 50 mM +0.7 -0.1 +0.8 +1.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.7 +0.5 -0.4 +2.1 -0.2
pH pH 9 +0.6 -0.2 +1.1 +0.5 +1.0
pH pH 6 +0.8 +0.1 +0.8 +1.0 +0.8
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