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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2547 and PP_2548 are separated by 243 nucleotides
PP_2548 and PP_2549 are separated by 14 nucleotides
PP_2549 and PP_2550 are separated by 97 nucleotides
PP_2550 and PP_2551 are separated by 137 nucleotides
PP_2547: PP_2547 - Transcriptional regulator, LysR family, at 2,891,898 to 2,892,767
_2547
PP_2548: PP_2548 - putative Sugar MFS transporter, at 2,893,011 to 2,894,327
_2548
PP_2549: PP_2549 - conserved protein of unknown function, at 2,894,342 to 2,895,733
_2549
PP_2550: PP_2550 - putative transcriptional regulatory protein, at 2,895,831 to 2,896,544
_2550
PP_2551: PP_2551 - Transcriptional regulator, LysR family, at 2,896,682 to 2,897,626
_2551
Group
Condition
PP
_2547
PP
_2548
PP
_2549
PP
_2550
PP
_2551
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM
+0.2
+0.1
+0.1
-3.1
-2.9
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and Parachlorophenylalanine 10 mM
-0.4
+0.1
+0.4
-2.4
-2.9
micoeukaryotes
Tetrahymena sp., Mixed culture-2
-0.9
-0.3
-0.4
-1.7
-0.4
carbon source
ferulate (C); with MOPS
-0.0
-0.0
-0.5
-1.9
-0.3
stress
R2A with Fusaric 240 ug/mL
-0.5
-0.2
-0.6
-1.5
+0.1
pH
Growth at pH6 and (C) D-Glucose
-2.7
+0.3
+0.4
-0.4
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-1.1
-0.1
-0.0
-0.6
-0.5
phage
P. putida PUT 7 MOI 1
-1.5
+0.2
-0.2
-0.8
-0.0
micoeukaryotes
Acanthamoeba sp., muxed culture-2
-0.2
-0.4
-0.2
-1.4
-0.1
stress
Growth with Chloride 200 mM
+0.3
-0.4
+0.1
-1.6
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.4
-0.6
-0.1
-1.1
+0.4
carbon source
carbon source D-Glucose 55.5 mM
+0.0
-0.0
+0.8
-2.9
+0.4
phage
P. putida Sci small MOI 0.1
+0.1
+0.5
-0.8
-0.5
-1.0
nitrogen source
nitrate (N)
-1.1
-0.3
-0.1
+0.4
-0.6
temperature shift
Temperature shift 30_to_25; with MOPS
-0.7
-0.1
-0.2
-1.0
+0.5
nitrogen source
D-2-Aminobutyric (N)
+0.7
-0.3
-0.1
-1.4
-0.1
nitrogen source
D-2-Aminobutyric (N)
-1.0
-0.7
+0.4
+0.5
-0.2
carbon source
Valeric (C)
+0.8
+0.0
+0.2
-0.4
-1.0
phage
P. putida Sci small MOI 0.1
-1.1
+0.4
+0.7
-0.1
-0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.1
+0.3
-0.4
+0.8
-0.9
carbon source
carbon source Bisabolene 48.9 mM
+0.6
-0.2
+0.4
-1.2
+0.4
temperature
Growth at 25C
-0.9
+0.2
+0.3
+0.4
+0.7
carbon source
4-Aminobutyric 10 mM (C)
+0.3
+0.3
+0.5
+1.0
-0.6
pH
pH 8
+1.1
+0.0
+0.7
+0.2
+0.4
carbon source
Tween 20 (C)
+0.3
+0.4
+0.5
+0.9
+0.4
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
+0.4
-0.2
+1.0
+0.3
+1.2
carbon source
carbon source 3-O-methyl-glucose 50 mM
+0.7
-0.1
+0.8
+1.1
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.7
+0.5
-0.4
+2.1
-0.2
pH
pH 9
+0.6
-0.2
+1.1
+0.5
+1.0
pH
pH 6
+0.8
+0.1
+0.8
+1.0
+0.8
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