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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2509 and PP_2510 are separated by 1658 nucleotides
PP_2510 and PP_2511 are separated by 125 nucleotides
PP_2511 and PP_2512 overlap by 16 nucleotides
PP_2512 and PP_5535 are separated by 35 nucleotides
PP_2509: PP_2509 - conserved protein of unknown function, at 2,856,567 to 2,856,731
_2509
PP_2510: PP_2510 - conserved protein of unknown function, at 2,858,390 to 2,858,644
_2510
PP_2511: PP_2511 - conserved protein of unknown function, at 2,858,770 to 2,859,183
_2511
PP_2512: PP_2512 - GTP cyclohydrolase 1, at 2,859,168 to 2,859,728
_2512
PP_5535: PP_5535 - conserved membrane protein of unknown function, at 2,859,764 to 2,860,222
_5535
Group
Condition
PP
_2509
PP
_2510
PP
_2511
PP
_2512
PP
_5535
pH
Growth at pH7 and (C) D-Glucose
-0.8
-4.7
-0.9
-0.1
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.5
-4.9
-1.0
+0.0
-0.3
pH
Growth at pH7 and (C) Trisodium citrate
-0.7
-4.5
-0.2
+0.0
+0.3
pH
Growth at pH6 and (C) Trisodium citrate
-0.3
-4.4
-0.5
+0.1
+0.2
phage
PUT 8 MOI 1
-0.5
-4.8
+0.1
+0.3
+0.1
temperature shift
Temperature shift 30_to_21; with MOPS
-0.3
-2.8
-0.4
-0.3
-0.2
phage
P. putida PUT 7 MOI 1
+0.7
+0.1
-4.5
-0.1
+0.3
carbon source
Tween 20 (C)
-0.7
-0.7
-1.2
-0.4
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.4
-1.6
-0.7
-0.8
+0.1
pH
Valerolactone (C) pH 6.5
+0.2
-2.7
-0.3
+0.1
-0.4
carbon source
L-Leucine (C)
+0.3
-2.4
-0.5
-0.2
-0.4
carbon source
Valerolactone (C)
-0.4
+0.1
-1.9
-0.2
-0.7
carbon source
1,3-Butandiol (C)
+0.1
-2.1
-0.8
+0.0
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.4
-2.6
-0.6
+0.3
+0.2
carbon source
ferulate (C); with MOPS
+0.5
-3.3
+0.1
+0.1
-0.2
phage
P. putida Sci small MOI 0.1
-0.1
+1.3
-5.3
+0.7
+0.6
pH
pH 4
+0.2
-2.0
-0.3
-0.4
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.9
-2.9
+0.3
+0.3
+0.4
nitrogen source
NAG (N); with MOPS
-0.5
+0.3
-1.0
-0.5
-1.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
-3.5
+0.6
+0.2
+0.0
pH
Growth at pH8 and (C) D-Glucose
+0.0
-3.1
+0.3
-0.1
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.3
-1.0
-1.2
+0.4
-0.8
phage
P. putida South MOI 10
-1.1
-1.4
+0.7
-0.1
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-1.5
-0.7
+0.4
-0.2
+0.6
stress
Growth with Chloride 200 mM; with MOPS
+0.3
-1.8
-0.5
+0.5
+0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.7
+1.1
+0.1
-0.7
nitrogen source
D-2-Aminobutyric (N)
-0.6
-1.8
+1.6
-0.3
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.5
-1.4
+1.2
+0.0
-0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+1.0
+1.1
-2.1
+0.2
-0.0
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
+0.5
-0.6
+1.4
+0.6
+0.3
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