Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5534 and PP_2509 overlap by 4 nucleotidesPP_2509 and PP_2510 are separated by 1658 nucleotidesPP_2510 and PP_2511 are separated by 125 nucleotidesPP_2511 and PP_2512 overlap by 16 nucleotides PP_5534: PP_5534 - protein of unknown function, at 2,856,340 to 2,856,570 _5534 PP_2509: PP_2509 - conserved protein of unknown function, at 2,856,567 to 2,856,731 _2509 PP_2510: PP_2510 - conserved protein of unknown function, at 2,858,390 to 2,858,644 _2510 PP_2511: PP_2511 - conserved protein of unknown function, at 2,858,770 to 2,859,183 _2511 PP_2512: PP_2512 - GTP cyclohydrolase 1, at 2,859,168 to 2,859,728 _2512
Group Condition PP_5534 PP_2509 PP_2510 PP_2511 PP_2512
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 -0.5 -4.9 -1.0 +0.0
pH Growth at pH7 and (C) D-Glucose +0.2 -0.8 -4.7 -0.9 -0.1
pH Growth at pH7 and (C) Trisodium citrate +0.1 -0.7 -4.5 -0.2 +0.0
pH Growth at pH6 and (C) Trisodium citrate +0.1 -0.3 -4.4 -0.5 +0.1
temperature shift Temperature shift 30_to_21; with MOPS -0.2 -0.3 -2.8 -0.4 -0.3
carbon source Tween 20 (C) -0.9 -0.7 -0.7 -1.2 -0.4
phage PUT 8 MOI 1 +1.0 -0.5 -4.8 +0.1 +0.3
pH Valerolactone (C) pH 6.5 -0.6 +0.2 -2.7 -0.3 +0.1
phage P. putida Sci small MOI 0.1 +0.1 -0.1 +1.3 -5.3 +0.7
phage P. putida PUT 7 MOI 1 +0.6 +0.7 +0.1 -4.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.4 -2.6 -0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 -0.4 -1.6 -0.7 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.9 -2.9 +0.3 +0.3
carbon source L-Leucine (C) -0.2 +0.3 -2.4 -0.5 -0.2
carbon source 1,3-Butandiol (C) -0.2 +0.1 -2.1 -0.8 +0.0
carbon source Trisodium citrate (C) -0.5 -0.1 +0.2 -1.3 -1.1
pH Growth at pH8 and (C) D-Glucose +0.0 +0.0 -3.1 +0.3 -0.1
carbon source ferulate (C); with MOPS +0.1 +0.5 -3.3 +0.1 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.0 -3.5 +0.6 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.3 -1.5 -0.7 +0.4 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 +0.3 -1.0 -1.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.6 -0.1 -2.4 +0.1 -0.1
pH pH 4 +0.4 +0.2 -2.0 -0.3 -0.4
phage P. putida South MOI 10 -0.1 -1.1 -1.4 +0.7 -0.1
stress Growth with Chloride 200 mM; with MOPS -0.0 +0.3 -1.8 -0.5 +0.5
nitrogen source D-2-Aminobutyric (N) +0.4 -0.6 -1.8 +1.6 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 +1.0 +1.1 -2.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.5 -1.4 +1.2 +0.0
stress R2A with Polymyxin B sulfate 0.001 mg/ml +0.1 +0.5 -0.6 +1.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.7 +1.9 +0.0 +0.3
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