Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2474 and PP_2475 are separated by 390 nucleotidesPP_2475 and PP_2476 are separated by 112 nucleotidesPP_2476 and PP_5528 are separated by 120 nucleotidesPP_5528 and PP_2477 are separated by 99 nucleotides PP_2474: PP_2474 - Glutathione S-transferase family protein, at 2,820,711 to 2,821,331 _2474 PP_2475: PP_2475 - Transcriptional regulator, TetR family, at 2,821,722 to 2,822,315 _2475 PP_2476: PP_2476 - NADPH-dependent curcumin/dihydrocurcumin reductase, at 2,822,428 to 2,823,462 _2476 PP_5528: PP_5528 - conserved protein of unknown function, at 2,823,583 to 2,823,825 _5528 PP_2477: PP_2477 - isoquinoline 1-oxidoreductase subunit alpha, at 2,823,925 to 2,824,386 _2477
Group Condition PP_2474 PP_2475 PP_2476 PP_5528 PP_2477
micoeukaryotes Tetrahymena sp., Mixed culture-2 -1.4 -0.5 -0.3 -3.3 -0.3
temperature Growth at 25C -1.8 -1.5 +0.1 -1.9 -0.4
carbon source 3-methyl-1-butanol (C) -1.0 -4.3 +0.0 +0.1 +0.0
nitrogen source NAG (N); with MOPS -2.3 -1.2 -0.2 -0.8 -0.6
carbon source 3-methyl-1-butanol (C) -0.3 -4.5 +0.1 -0.1 -0.0
carbon source 4-Methyl-2-oxopentanoic (C) -0.3 -1.5 -0.1 -2.8 +0.2
nitrogen source Ammonium chloride and TNT (N) -0.2 -3.7 +0.0 -0.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -2.1 -0.6 +0.2 -2.0 +0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.5 -0.4 +0.1 -4.1 -0.1
nitrogen source D-2-Aminobutyric (N) -0.4 -0.4 +0.2 -3.2 -0.1
nitrogen source Ammonium chloride and TNT (N) +0.4 -3.6 +0.0 -0.4 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.1 -0.8 -0.0 -2.8 +0.0
carbon source acetate (C) -3.0 -1.1 -0.1 +1.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -1.1 -0.2 -1.8 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.7 -2.8 -0.1 +0.4 -0.2
nitrogen source Ammonium chloride and TNT (N) +0.5 -2.6 +0.0 -0.1 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -3.3 -0.1 -0.2 +0.5 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.7 -2.2 +0.1 -1.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -1.2 +0.0 -2.1 +0.4
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.5 -4.3 +0.3 +0.7 +0.4
carbon source D-Glucose (C); with MOPS +0.6 -1.2 -0.0 -1.8 +0.3
phage P. putida PUT 7 MOI 1 +0.6 -0.4 -0.0 -2.7 +0.5
nitrogen source 2,6-diaminopimelic (N) +0.3 +0.5 +0.3 -2.6 -0.1
phage P. putida Sci Big MOI 0.1 -0.4 +1.0 +0.6 -2.8 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.1 -1.9 -0.0 +1.2 -0.5
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.4 +1.4 -0.0 -2.2 -0.6
pH Growth at pH9 and (C) Trisodium citrate +1.3 -0.9 -0.0 +1.0 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.5 -1.0 +0.1 +2.3 +0.4
carbon source Valerolactone (C) +0.1 -0.1 +0.3 +3.1 -0.4
carbon source Valerolactone (C) -0.3 -0.0 +0.0 +3.6 +0.0
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