Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2469 and PP_2470 are separated by 31 nucleotidesPP_2470 and PP_2471 are separated by 4 nucleotidesPP_2471 and PP_2472 overlap by 20 nucleotidesPP_2472 and PP_t44 are separated by 114 nucleotides PP_2469: PP_2469 - Phenylalanine--tRNA ligase alpha subunit, at 2,812,816 to 2,813,832 _2469 PP_2470: PP_2470 - Phenylalanine--tRNA ligase beta subunit, at 2,813,864 to 2,816,245 _2470 PP_2471: PP_2471 - Integration host factor subunit alpha, at 2,816,250 to 2,816,552 _2471 PP_2472: PP_2472 - putative transcriptional regulator, MerR family, at 2,816,533 to 2,816,889 _2472 PP_t44: PP_t44 - tRNA-Pro, at 2,817,004 to 2,817,080 _t44
Group Condition PP_2469 PP_2470 PP_2471 PP_2472 PP_t44
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.6 N.D.
stress R2A with Fusaric 480 ug/mL N.D. N.D. N.D. -1.4 N.D.
temperature shift Temperature shift 10_to_30; with MOPS N.D. N.D. N.D. -1.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.2 N.D.
temperature shift Temperature shift 30_to_21; with MOPS N.D. N.D. N.D. -1.1 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. N.D. -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.1 N.D.
pH Growth at pH6 and (C) D-Glucose N.D. N.D. N.D. -1.1 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs N.D. N.D. N.D. -1.0 N.D.
stress R2A with Fusaric 480 ug/mL N.D. N.D. N.D. -1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.0 N.D.
nitrogen source DL-2-Aminobutyric (N) N.D. N.D. N.D. -0.9 N.D.
stress R2A with Fusaric 480 ug/mL N.D. N.D. N.D. -0.9 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. N.D. N.D. -0.9 N.D.
phage P. putida South MOI 0.1 N.D. N.D. N.D. +0.9 N.D.
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay N.D. N.D. N.D. +0.9 N.D.
phage PUT 8 MOI 1 N.D. N.D. N.D. +0.9 N.D.
phage P. put South MOI 1 N.D. N.D. N.D. +0.9 N.D.
phage P. putida PUT10 MOI 1 N.D. N.D. N.D. +0.9 N.D.
phage P. putida PUT10 MOI 10 N.D. N.D. N.D. +0.9 N.D.
phage P. put PUT 10 MOI 10 N.D. N.D. N.D. +1.0 N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay N.D. N.D. N.D. +1.0 N.D.
phage P. putida PUT10 MOI 0.1 N.D. N.D. N.D. +1.0 N.D.
phage PUT8 MOI 10 N.D. N.D. N.D. +1.0 N.D.
carbon source Valerolactone (C) N.D. N.D. N.D. +1.0 N.D.
nitrogen source Propandiamine (N) N.D. N.D. N.D. +1.1 N.D.
phage P. putida South MOI 1 N.D. N.D. N.D. +1.1 N.D.
phage P. putida PUT10 MOI 10 N.D. N.D. N.D. +1.1 N.D.
carbon source Valerolactone (C) N.D. N.D. N.D. +1.2 N.D.
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