Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2461 and PP_2462 are separated by 220 nucleotidesPP_2462 and PP_2463 are separated by 200 nucleotidesPP_2463 and PP_5525 are separated by 162 nucleotidesPP_5525 and PP_2465 are separated by 338 nucleotides PP_2461: PP_2461 - conserved protein of unknown function, at 2,807,567 to 2,807,779 _2461 PP_2462: PP_2462 - conserved protein of unknown function, at 2,808,000 to 2,808,374 _2462 PP_2463: PP_2463 - nucleic acid cold-shock chaperone, at 2,808,575 to 2,808,787 _2463 PP_5525: PP_5525 - conserved exported protein of unknown function, at 2,808,950 to 2,809,255 _5525 PP_2465: PP_2465 - Threonine--tRNA ligase, at 2,809,594 to 2,811,516 _2465
Group Condition PP_2461 PP_2462 PP_2463 PP_5525 PP_2465
pH Growth at pH7 and (C) Trisodium citrate N.D. +0.7 N.D. -3.2 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. -1.0 N.D. -1.3 N.D.
temperature shift Temperature shift 10_to_30; with MOPS N.D. -1.6 N.D. -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.4 N.D. -1.6 N.D.
temperature shift Temperature shift 30_to_21; with MOPS N.D. -1.2 N.D. -0.8 N.D.
nitrogen source Urea (N) N.D. -2.5 N.D. +0.7 N.D.
stress 3-methyl-1-butanol 0.4 vol% N.D. -0.8 N.D. -1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.7 N.D. -0.0 N.D.
pH Growth at pH6 and (C) D-Glucose N.D. -2.5 N.D. +0.8 N.D.
carbon source Nonanoic (C) N.D. -0.4 N.D. -1.3 N.D.
carbon source Myristic (C) N.D. -2.2 N.D. +0.8 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs N.D. +0.3 N.D. -1.5 N.D.
stress Growth with Chloride 500 mM N.D. -1.6 N.D. +0.5 N.D.
pH Growth at pH7 and (C) D-Glucose N.D. -1.4 N.D. +0.5 N.D.
carbon source D-Glucose (C) N.D. -1.3 N.D. +0.4 N.D.
carbon source Valerolactone (C) N.D. -1.4 N.D. +0.5 N.D.
temperature shift Temperature shift 10_to_25; with MOPS N.D. -1.4 N.D. +0.6 N.D.
carbon source carbon source 4-vinylphenol 0.1 mM N.D. +1.2 N.D. -1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.0 N.D. +0.8 N.D.
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. +1.1 N.D. -1.0 N.D.
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM N.D. +1.0 N.D. -0.7 N.D.
pH Growth at pH6 and (C) Trisodium citrate N.D. +1.3 N.D. -0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +1.2 N.D. -0.5 N.D.
nitrogen source D-2-Aminobutyric (N) N.D. +1.2 N.D. -0.5 N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +1.4 N.D. -0.6 N.D.
nitrogen source D-2-Aminobutyric (N) N.D. +1.9 N.D. -0.4 N.D.
phage P. putida South MOI 0.1 N.D. +1.2 N.D. +0.7 N.D.
phage P. putida South MOI 0.1 N.D. +1.5 N.D. +0.6 N.D.
phage P. putida PUT10 MOI 1 N.D. +1.8 N.D. +0.6 N.D.
phage P. putida PUT10 MOI 10 N.D. +1.8 N.D. +0.7 N.D.
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