Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2439 and PP_2440 are separated by 124 nucleotidesPP_2440 and PP_2441 are separated by 166 nucleotidesPP_2441 and PP_2442 are separated by 4 nucleotidesPP_2442 and PP_2443 are separated by 88 nucleotides PP_2439: PP_2439 - peroxiredoxin, alkylhydroperoxide reductase - small subunit, at 2,786,990 to 2,787,553 _2439 PP_2440: PP_2440 - Alkyl hydroperoxide reductase subunit F, at 2,787,678 to 2,789,240 _2440 PP_2441: PP_2441 - conserved protein of unknown function, at 2,789,407 to 2,789,595 _2441 PP_2442: PP_2442 - conserved protein of unknown function, DUF480 family, at 2,789,600 to 2,790,247 _2442 PP_2443: PP_2443 - Serine/threonine transporter SstT, at 2,790,336 to 2,791,547 _2443
Group Condition PP_2439 PP_2440 PP_2441 PP_2442 PP_2443
nitrogen source Ammonium chloride (N) -0.3 +0.1 -0.6 -5.0 +0.3
phage PUT 8 MOI 1 +0.2 +0.4 -0.4 -5.2 -0.5
carbon source DL-3-Hydroxybutyric (C) -1.1 -0.2 -0.6 -3.1 -0.2
pH pH 8 -0.5 -0.6 -0.9 -2.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 -0.5 +0.1 -4.0 -0.1
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -1.4 -0.2 -0.8 -1.7 -0.0
stress MOPS minimal media_Glucose20mM with Chloride 200 mM -1.3 -0.5 -0.5 -1.7 +0.1
stress MOPS minimal media_Glucose20mM with Poly(3-hydroxybutyric) 0.1 mg/mL -1.4 -0.1 -0.8 -1.4 -0.1
pH D-Glucose (C) pH 7 -1.3 -0.2 -0.0 -2.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.4 -0.8 -2.3 -0.2
pH D-Glucose (C) pH 6.5 -1.9 -0.2 -0.2 -1.3 -0.1
carbon source carbon source D-Glucose 55.5 mM -0.5 -0.2 -1.9 -0.6 -0.4
pH D-Glucose (C) pH 7.5 -1.6 -0.3 -0.6 -1.3 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 -0.9 +0.1 -1.7 -0.4
pH Growth at pH6 and (C) D-Glucose -0.8 -0.1 -1.8 -1.2 +0.3
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM -1.6 -0.3 -0.4 -1.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -3.9 -0.2 -0.1 +1.3 -0.3
nitrogen source 2,6-diaminopimelic (N) -0.0 -0.0 -4.1 +0.5 +0.4
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL -1.8 -0.1 +0.1 -1.5 +0.1
stress MOPS minimal media_Glucose20mM with Lithium chloride 200 mM -1.3 -0.1 +0.0 -1.9 +0.2
carbon source DL-3-Hydroxyisobutyric (C) -1.9 +0.0 +0.5 -1.7 +0.1
nitrogen source D-2-Aminobutyric (N) -1.4 +0.1 -1.2 -0.9 +0.4
no stress control Growth with no stress +0.1 +0.2 +0.1 -3.3 +0.1
carbon source 3-methyl-1-butanol 0.1 vol% (C) -2.5 +0.4 -0.5 +0.5 -0.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.6 -0.3 +0.9 -2.1 -0.0
attachment on pdms attachment on PDMS; 2 hours +0.2 -0.2 +0.5 -2.6 -0.2
nitrogen source NAG (N); with MOPS +0.2 +0.5 -0.5 -2.8 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.6 +0.3 -0.6 +0.8 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.9 -0.5 +0.8 +0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.3 +2.8 -0.0 +0.4
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