Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2404 and PP_2405 overlap by 11 nucleotidesPP_2405 and PP_2406 are separated by 113 nucleotidesPP_2406 and PP_2407 overlap by 4 nucleotidesPP_2407 and PP_2408 are separated by 238 nucleotides PP_2404: PP_2404 - conserved exported protein of unknown function, at 2,748,726 to 2,749,001 _2404 PP_2405: PP_2405 - conserved exported protein of unknown function, at 2,748,991 to 2,749,527 _2405 PP_2406: PP_2406 - putative quinate/shikimate dehydrogenase, at 2,749,641 to 2,750,489 _2406 PP_2407: PP_2407 - 3-dehydroquinate dehydratase 2, type II, at 2,750,486 to 2,750,935 _2407 PP_2408: PP_2408 - cobalt-zinc-cadmium resistance protein, at 2,751,174 to 2,752,451 _2408
Group Condition PP_2404 PP_2405 PP_2406 PP_2407 PP_2408
carbon source Quinic Acid (C) -0.2 -0.2 -2.0 N.D. -0.1
carbon source Quinic Acid (C) -0.2 +0.0 -2.1 N.D. -0.2
carbon source Myristic (C) -1.3 +0.1 -0.6 N.D. -0.4
carbon source carbon source D-Glucose 55.5 mM -0.1 -0.2 -0.8 N.D. -1.1
nitrogen source Urea (N) -1.5 -0.1 -0.3 N.D. -0.1
phage P. putida Sci small MOI 0.1 -1.2 -0.9 -0.1 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.7 +0.1 -0.9 N.D. -0.4
nitrogen source D-2-Aminobutyric (N) -1.1 -0.4 +0.4 N.D. -0.8
pH Growth at pH8 and (C) D-Glucose -1.3 -0.0 -0.2 N.D. -0.2
pH Growth at pH6 and (C) D-Glucose -1.2 -0.4 -0.6 N.D. +0.4
phage P. putida PUT 7 MOI 1 -0.1 +0.0 -1.3 N.D. -0.2
nitrogen source no extra (N); with MOPS -1.1 -0.1 +0.3 N.D. -0.6
nitrogen source Propiolactam (N) -0.9 -0.6 +0.1 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.4 -0.5 N.D. -0.2
phage P. putida Sci small MOI 0.1 -0.5 -0.1 -0.7 N.D. +0.4
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.7 +0.2 +0.4 N.D. -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.9 -0.2 +0.4 N.D. -0.1
carbon source ferulate (C); with MOPS +0.6 -0.4 -1.3 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.5 -0.5 -0.3 N.D. -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.5 -0.3 -0.3 N.D. +0.5
nitrogen source nitrogen source nitrite 0.5mM +0.8 -0.3 -0.4 N.D. -0.7
pH pH 7 +1.0 -0.3 -0.1 N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.7 -0.3 -0.5 N.D. -0.6
nitrogen source nitrogen source nitrite 1mM +0.9 -0.1 +0.2 N.D. -0.6
nitrogen source nitrogen source nitrate 20mM +0.6 +0.3 +0.4 N.D. -0.4
temperature shift Temperature shift 30_to_25; with MOPS +0.8 -0.4 +0.3 N.D. +0.3
stress R2A with Fusaric 480 ug/mL +0.9 +0.5 -0.4 N.D. -0.0
carbon source Tween 20 (C) +0.5 +0.4 -0.3 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 -0.2 +1.4 N.D. -0.1
carbon source L-Leucine (C) +0.0 +0.6 +0.7 N.D. +0.7
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