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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2306 and PP_2307 are separated by 14 nucleotides
PP_2307 and PP_2308 are separated by 77 nucleotides
PP_2308 and PP_2309 are separated by 41 nucleotides
PP_2309 and PP_2310 are separated by 75 nucleotides
PP_2306: PP_2306 - conserved exported protein of unknown function, at 2,636,745 to 2,637,023
_2306
PP_2307: PP_2307 - conserved protein of unknown function, at 2,637,038 to 2,637,817
_2307
PP_2308: PP_2308 - putative Acyl-CoA thioesterase II, at 2,637,895 to 2,638,692
_2308
PP_2309: PP_2309 - conserved protein of unknown function, at 2,638,734 to 2,639,102
_2309
PP_2310: PP_2310 - Methyl-accepting chemotaxis transducer, at 2,639,178 to 2,640,656
_2310
Group
Condition
PP
_2306
PP
_2307
PP
_2308
PP
_2309
PP
_2310
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.5
-4.1
-0.5
-2.0
+0.2
nitrogen source
nitrate (N)
-0.0
-2.2
-0.0
-5.7
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.3
-3.8
-0.1
-3.9
+0.2
nitrogen source
NAG (N); with MOPS
-0.4
-6.9
+0.2
-0.6
+0.0
carbon source
Trisodium citrate (C)
-0.0
-1.1
+0.1
-5.7
-0.6
nitrogen source
NAG (N); with MOPS
+0.4
-0.8
+0.2
-5.7
-0.6
temperature shift
Temperature shift 30_to_25
-0.2
-6.8
+0.2
+0.5
+0.1
temperature
Growth at 30C; with MOPS
+0.7
-0.2
-0.2
-5.7
+0.1
pH
Growth at pH6 and (C) D-Glucose
-1.6
-2.0
+0.2
-1.8
-0.1
phage
P. putida Sci small MOI 0.1
-1.0
+1.3
+0.3
-4.9
-0.5
carbon source
Valerolactone (C)
+0.1
-4.0
+0.2
-1.0
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-1.6
-0.2
-0.1
-2.7
+0.1
carbon source
Crotonic (C)
-0.6
-1.8
-0.3
-1.4
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-1.5
+0.0
-2.8
-0.3
carbon source
Glutaric (C)
-0.9
-2.4
-0.1
-1.1
+0.1
temperature shift
Temperature shift 30_to_21; with MOPS
-4.9
-0.5
+0.3
+1.3
-0.1
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-1.4
-1.9
-0.6
+0.3
-0.2
pH
Growth at pH6 and (C) Trisodium citrate
-3.6
+0.1
+0.5
-0.2
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.1
-0.9
-0.2
-2.0
+0.9
carbon source
3-Hydroxydecanoic (C)
-2.1
-1.6
-0.1
+0.6
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
+0.9
-3.8
+0.4
-0.6
+0.1
phage
P. put South MOI 1
-0.3
+0.6
+0.2
-3.1
+0.2
phage
P. putida Sci Big MOI 0.1
-3.5
+0.6
+0.7
+0.6
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-2.3
+0.5
+0.5
-0.8
+0.3
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
-2.1
-0.8
+0.1
+1.4
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-2.8
+0.2
+0.4
+1.2
+0.2
carbon source
Myristic (C)
-3.4
+0.6
+0.8
+1.3
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
+1.3
-2.6
+0.2
+0.6
+0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+2.2
-0.7
+0.1
-1.7
+0.8
nitrogen source
D-2-Aminobutyric (N)
+1.5
+2.0
+0.9
-0.0
-0.1
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