Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2306 and PP_2307 are separated by 14 nucleotidesPP_2307 and PP_2308 are separated by 77 nucleotidesPP_2308 and PP_2309 are separated by 41 nucleotidesPP_2309 and PP_2310 are separated by 75 nucleotides PP_2306: PP_2306 - conserved exported protein of unknown function, at 2,636,745 to 2,637,023 _2306 PP_2307: PP_2307 - conserved protein of unknown function, at 2,637,038 to 2,637,817 _2307 PP_2308: PP_2308 - putative Acyl-CoA thioesterase II, at 2,637,895 to 2,638,692 _2308 PP_2309: PP_2309 - conserved protein of unknown function, at 2,638,734 to 2,639,102 _2309 PP_2310: PP_2310 - Methyl-accepting chemotaxis transducer, at 2,639,178 to 2,640,656 _2310
Group Condition PP_2306 PP_2307 PP_2308 PP_2309 PP_2310
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.5 -4.1 -0.5 -2.0 +0.2
nitrogen source nitrate (N) -0.0 -2.2 -0.0 -5.7 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 -3.8 -0.1 -3.9 +0.2
nitrogen source NAG (N); with MOPS -0.4 -6.9 +0.2 -0.6 +0.0
carbon source Trisodium citrate (C) -0.0 -1.1 +0.1 -5.7 -0.6
nitrogen source NAG (N); with MOPS +0.4 -0.8 +0.2 -5.7 -0.6
temperature shift Temperature shift 30_to_25 -0.2 -6.8 +0.2 +0.5 +0.1
temperature Growth at 30C; with MOPS +0.7 -0.2 -0.2 -5.7 +0.1
pH Growth at pH6 and (C) D-Glucose -1.6 -2.0 +0.2 -1.8 -0.1
phage P. putida Sci small MOI 0.1 -1.0 +1.3 +0.3 -4.9 -0.5
carbon source Valerolactone (C) +0.1 -4.0 +0.2 -1.0 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.6 -0.2 -0.1 -2.7 +0.1
carbon source Crotonic (C) -0.6 -1.8 -0.3 -1.4 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -1.5 +0.0 -2.8 -0.3
carbon source Glutaric (C) -0.9 -2.4 -0.1 -1.1 +0.1
temperature shift Temperature shift 30_to_21; with MOPS -4.9 -0.5 +0.3 +1.3 -0.1
stress R2A with Polymyxin B sulfate 0.001 mg/ml -1.4 -1.9 -0.6 +0.3 -0.2
pH Growth at pH6 and (C) Trisodium citrate -3.6 +0.1 +0.5 -0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.1 -0.9 -0.2 -2.0 +0.9
carbon source 3-Hydroxydecanoic (C) -2.1 -1.6 -0.1 +0.6 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.9 -3.8 +0.4 -0.6 +0.1
phage P. put South MOI 1 -0.3 +0.6 +0.2 -3.1 +0.2
phage P. putida Sci Big MOI 0.1 -3.5 +0.6 +0.7 +0.6 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.3 +0.5 +0.5 -0.8 +0.3
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM -2.1 -0.8 +0.1 +1.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.8 +0.2 +0.4 +1.2 +0.2
carbon source Myristic (C) -3.4 +0.6 +0.8 +1.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs +1.3 -2.6 +0.2 +0.6 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.2 -0.7 +0.1 -1.7 +0.8
nitrogen source D-2-Aminobutyric (N) +1.5 +2.0 +0.9 -0.0 -0.1
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