Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2290 and PP_2291 overlap by 14 nucleotidesPP_2291 and PP_2292 are separated by 7 nucleotidesPP_2292 and PP_2293 are separated by 148 nucleotidesPP_2293 and PP_2294 are separated by 36 nucleotides PP_2290: PP_2290 - conserved protein of unknown function, at 2,618,024 to 2,618,260 _2290 PP_2291: PP_2291 - conserved protein of unknown function, at 2,618,247 to 2,618,534 _2291 PP_2292: PP_2292 - conserved protein of unknown function, at 2,618,542 to 2,619,312 _2292 PP_2293: PP_2293 - DNA maturase B, at 2,619,461 to 2,621,149 _2293 PP_2294: PP_2294 - conserved exported protein of unknown function, at 2,621,186 to 2,621,626 _2294
Group Condition PP_2290 PP_2291 PP_2292 PP_2293 PP_2294
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -2.1 +0.1 -0.2 -0.2
nitrogen source nitrate (N) -0.4 -1.9 -0.0 -0.1 +0.3
stress R2A with Fusaric 480 ug/mL -1.0 -1.0 +0.1 +0.1 +0.1
carbon source Myristic (C) -0.9 -1.7 +0.6 +0.3 +0.2
pH Growth at pH6 and (C) Trisodium citrate +0.5 -2.0 +0.0 -0.0 +0.0
pH pH 5 +0.4 -0.6 -0.9 +0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.3 +0.5 -0.1 -0.2 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.6 -0.6 -0.5 -0.4 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.5 +0.3 +0.0 -0.0 +0.4
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.8 -0.7 +0.4 +0.2 +0.3
nitrogen source 2,6-diaminopimelic (N) +0.4 -1.1 +0.3 +0.4 -0.4
carbon source ferulate (C); with MOPS +1.0 -1.2 -0.0 +0.1 -0.2
carbon source Tween 20 (C) +0.6 -0.5 -0.3 -0.4 +0.4
phage P. putida South MOI 10 +0.3 -1.1 -0.1 +0.5 +0.4
phage PUT8 MOI 0.1 -0.6 -0.4 +0.7 +0.1 +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 +1.1 -0.2 +0.2 -0.1
pH Growth at pH6 and (C) D-Glucose -0.9 +0.9 -0.0 +0.3 +0.4
phage PUT 8 MOI 1 +0.7 -0.2 +0.9 -0.4 -0.2
stress Growth with Chloride 200 mM; with MOPS +0.5 +0.9 -0.7 +0.1 +0.2
carbon source Valerolactone (C) +0.9 -0.6 +0.4 +0.4 +0.1
temperature shift Temperature shift 30_to_21; with MOPS +0.6 +0.9 -0.2 +0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +1.1 +0.5 -0.4 -0.3
stress Growth with Chloride 200 mM +0.7 +0.9 -0.4 +0.2 +0.0
pH Growth at pH9 and (C) Trisodium citrate +0.1 +1.5 +0.5 +0.2 -0.6
phage P. putida Sci small MOI 0.1 -0.3 +0.6 +0.5 +0.8 +0.3
phage P. putida Missley MOI 0.1 +0.3 +0.9 -0.4 +0.6 +0.6
phage PUT8 MOI 10 +0.3 +0.8 +0.7 +0.2 +0.3
pH Growth at pH9 and (C) D-Glucose +1.0 +1.0 +0.0 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +1.9 +0.6 +0.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.4 +0.7 +0.7 -0.3 +0.1
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