Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5505 and PP_2264 are separated by 39 nucleotidesPP_2264 and PP_2265 are separated by 126 nucleotidesPP_2265 and PP_t37 are separated by 269 nucleotidesPP_t37 and PP_t38 are separated by 41 nucleotides PP_5505: PP_5505 - conserved transmembrane protein of unknown function, at 2,582,462 to 2,582,734 _5505 PP_2264: PP_2264 - putative Sugar ABC transporter, periplasmic sugar-binding protein, at 2,582,774 to 2,584,513 _2264 PP_2265: PP_2265 - methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 2,584,640 to 2,585,494 _2265 PP_t37: PP_t37 - tRNA-Arg, at 2,585,764 to 2,585,840 _t37 PP_t38: PP_t38 - tRNA-His, at 2,585,882 to 2,585,957 _t38
Group Condition PP_5505 PP_2264 PP_2265 PP_t37 PP_t38
pH Growth at pH7 and (C) Trisodium citrate -4.1 -0.2 N.D. N.D. N.D.
nitrogen source Propandiamine (N) -2.4 -0.2 N.D. N.D. N.D.
temperature shift Temperature shift 10_to_30 -2.5 -0.1 N.D. N.D. N.D.
pH Growth at pH9 and (C) Trisodium citrate -2.6 +0.1 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -2.1 -0.0 N.D. N.D. N.D.
carbon source L-Leucine (C) -2.0 +0.2 N.D. N.D. N.D.
pH Growth at pH9 and (C) Trisodium citrate -1.8 -0.0 N.D. N.D. N.D.
nitrogen source nitrite (N) -1.6 -0.2 N.D. N.D. N.D.
phage P. putida Sci small MOI 0.1 -1.6 -0.1 N.D. N.D. N.D.
carbon source Trisodium citrate (C) -1.5 -0.1 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.5 +0.0 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.8 +0.3 N.D. N.D. N.D.
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.6 +0.2 N.D. N.D. N.D.
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -1.1 -0.3 N.D. N.D. N.D.
nitrogen source nitrate (N) -1.4 +0.0 N.D. N.D. N.D.
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay -1.3 -0.1 N.D. N.D. N.D.
pH Growth at pH6 and (C) Trisodium citrate -1.1 -0.3 N.D. N.D. N.D.
carbon source Valerolactone (C) -1.3 +0.0 N.D. N.D. N.D.
carbon source 1,3-Hexanediol (C) -1.0 -0.3 N.D. N.D. N.D.
temperature shift Temperature shift 10_to_21; with MOPS -1.1 -0.1 N.D. N.D. N.D.
carbon source 2,3-Butanediol (C) -1.1 -0.1 N.D. N.D. N.D.
pH Growth at pH6 and (C) D-Glucose -1.3 +0.1 N.D. N.D. N.D.
carbon source ferulate (C); with MOPS -1.2 +0.1 N.D. N.D. N.D.
carbon source acetate (C) -1.3 +0.2 N.D. N.D. N.D.
phage P. putida PUT10 MOI 1 -1.3 +0.3 N.D. N.D. N.D.
nitrogen source Urea (N) -0.9 +0.3 N.D. N.D. N.D.
phage P. putida Sci Big MOI 0.1 -0.7 +0.6 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.1 -0.1 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.0 +0.2 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.5 N.D. N.D. N.D.
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