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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_5505 and PP_2264 are separated by 39 nucleotides
PP_2264 and PP_2265 are separated by 126 nucleotides
PP_2265 and PP_t37 are separated by 269 nucleotides
PP_t37 and PP_t38 are separated by 41 nucleotides
PP_5505: PP_5505 - conserved transmembrane protein of unknown function, at 2,582,462 to 2,582,734
_5505
PP_2264: PP_2264 - putative Sugar ABC transporter, periplasmic sugar-binding protein, at 2,582,774 to 2,584,513
_2264
PP_2265: PP_2265 - methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 2,584,640 to 2,585,494
_2265
PP_t37: PP_t37 - tRNA-Arg, at 2,585,764 to 2,585,840
_t37
PP_t38: PP_t38 - tRNA-His, at 2,585,882 to 2,585,957
_t38
Group
Condition
PP
_5505
PP
_2264
PP
_2265
PP
_t37
PP
_t38
pH
Growth at pH7 and (C) Trisodium citrate
-4.1
-0.2
N.D.
N.D.
N.D.
nitrogen source
Propandiamine (N)
-2.4
-0.2
N.D.
N.D.
N.D.
temperature shift
Temperature shift 10_to_30
-2.5
-0.1
N.D.
N.D.
N.D.
pH
Growth at pH9 and (C) Trisodium citrate
-2.6
+0.1
N.D.
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-2.1
-0.0
N.D.
N.D.
N.D.
carbon source
L-Leucine (C)
-2.0
+0.2
N.D.
N.D.
N.D.
pH
Growth at pH9 and (C) Trisodium citrate
-1.8
-0.0
N.D.
N.D.
N.D.
nitrogen source
nitrite (N)
-1.6
-0.2
N.D.
N.D.
N.D.
phage
P. putida Sci small MOI 0.1
-1.6
-0.1
N.D.
N.D.
N.D.
carbon source
Trisodium citrate (C)
-1.5
-0.1
N.D.
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-1.5
+0.0
N.D.
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-1.8
+0.3
N.D.
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.6
+0.2
N.D.
N.D.
N.D.
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-1.1
-0.3
N.D.
N.D.
N.D.
nitrogen source
nitrate (N)
-1.4
+0.0
N.D.
N.D.
N.D.
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
-1.3
-0.1
N.D.
N.D.
N.D.
pH
Growth at pH6 and (C) Trisodium citrate
-1.1
-0.3
N.D.
N.D.
N.D.
carbon source
Valerolactone (C)
-1.3
+0.0
N.D.
N.D.
N.D.
carbon source
1,3-Hexanediol (C)
-1.0
-0.3
N.D.
N.D.
N.D.
temperature shift
Temperature shift 10_to_21; with MOPS
-1.1
-0.1
N.D.
N.D.
N.D.
carbon source
2,3-Butanediol (C)
-1.1
-0.1
N.D.
N.D.
N.D.
pH
Growth at pH6 and (C) D-Glucose
-1.3
+0.1
N.D.
N.D.
N.D.
carbon source
ferulate (C); with MOPS
-1.2
+0.1
N.D.
N.D.
N.D.
carbon source
acetate (C)
-1.3
+0.2
N.D.
N.D.
N.D.
phage
P. putida PUT10 MOI 1
-1.3
+0.3
N.D.
N.D.
N.D.
nitrogen source
Urea (N)
-0.9
+0.3
N.D.
N.D.
N.D.
phage
P. putida Sci Big MOI 0.1
-0.7
+0.6
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.1
-0.1
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.0
+0.2
N.D.
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.9
+0.5
N.D.
N.D.
N.D.
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