Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_23SF and PP_5SF are separated by 131 nucleotidesPP_5SF and PP_2240 are separated by 150 nucleotidesPP_2240 and PP_2241 are separated by 210 nucleotidesPP_2241 and PP_2242 are separated by 430 nucleotides PP_23SF: PP_23SF - 23S ribosomal RNA, at 2,550,717 to 2,553,618 _23SF PP_5SF: PP_5SF - 5S ribosomal RNA, at 2,553,750 to 2,553,869 _5SF PP_2240: PP_2240 - putative ABC efflux transporter, permease/ATP-binding protein, at 2,554,020 to 2,555,900 _2240 PP_2241: PP_2241 - putative major facilitator family transporter, at 2,556,111 to 2,557,310 _2241 PP_2242: PP_2242 - ferric enterobactin transport system outer membrane subunit, at 2,557,741 to 2,559,975 _2242
Group Condition PP_23SF PP_5SF PP_2240 PP_2241 PP_2242
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. N.D. -0.9 -0.0 +0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. N.D. -0.7 -0.3 +0.2
nitrogen source TNT (N) N.D. N.D. +0.3 -0.4 -0.1
temperature Growth at 30C; with MOPS N.D. N.D. -0.3 +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.3 -0.1 -0.3
phage P. putida South MOI 10 N.D. N.D. +0.3 -0.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.4 -0.0 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.5 +0.0 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs N.D. N.D. +0.4 -0.3 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. N.D. +0.2 -0.2 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. N.D. -0.2 +0.2 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. N.D. +0.5 -0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +0.2 -0.1 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. +0.4 +0.2 -0.1
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. N.D. +0.2 +0.4 -0.0
phage P. putida compost MOI 0.1 N.D. N.D. +0.3 +0.2 +0.2
carbon source carbon source 2-Deoxy-D-glucose 50 mM N.D. N.D. +0.4 +0.3 -0.0
stress R2A with Fusaric 480 ug/mL N.D. N.D. +0.4 +0.2 +0.1
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. N.D. N.D. +0.4 +0.1 +0.1
carbon source ferulate (C); with MOPS N.D. N.D. +0.2 -0.0 +0.6
phage P. putida Sci small MOI 0.1 N.D. N.D. +0.2 +0.3 +0.3
stress R2A with Fusaric 480 ug/mL N.D. N.D. +0.5 +0.2 +0.1
phage P. put South MOI 10 N.D. N.D. +0.4 +0.3 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. N.D. +0.4 +0.3 +0.2
pH pH 4 N.D. N.D. +0.2 +0.4 +0.2
phage P. putida Sci small MOI 0.1 N.D. N.D. +0.4 +0.1 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. N.D. +0.5 +0.4 +0.3
phage P. putida PUT 7 MOI 1 N.D. N.D. +0.7 +0.3 +0.2
phage P. putida Missley MOI 0.1 N.D. N.D. +0.4 +0.5 +0.4
phage P. putida Sci Big MOI 0.1 N.D. N.D. +0.6 +0.6 +0.3
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