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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_23SF and PP_5SF are separated by 131 nucleotides
PP_5SF and PP_2240 are separated by 150 nucleotides
PP_2240 and PP_2241 are separated by 210 nucleotides
PP_2241 and PP_2242 are separated by 430 nucleotides
PP_23SF: PP_23SF - 23S ribosomal RNA, at 2,550,717 to 2,553,618
_23SF
PP_5SF: PP_5SF - 5S ribosomal RNA, at 2,553,750 to 2,553,869
_5SF
PP_2240: PP_2240 - putative ABC efflux transporter, permease/ATP-binding protein, at 2,554,020 to 2,555,900
_2240
PP_2241: PP_2241 - putative major facilitator family transporter, at 2,556,111 to 2,557,310
_2241
PP_2242: PP_2242 - ferric enterobactin transport system outer membrane subunit, at 2,557,741 to 2,559,975
_2242
Group
Condition
PP
_23SF
PP
_5SF
PP
_2240
PP
_2241
PP
_2242
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
N.D.
-0.9
-0.0
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
N.D.
-0.7
-0.3
+0.2
nitrogen source
TNT (N)
N.D.
N.D.
+0.3
-0.4
-0.1
temperature
Growth at 30C; with MOPS
N.D.
N.D.
-0.3
+0.3
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
-0.1
-0.3
phage
P. putida South MOI 10
N.D.
N.D.
+0.3
-0.1
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.4
-0.0
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
+0.5
+0.0
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
N.D.
N.D.
+0.4
-0.3
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
N.D.
N.D.
+0.2
-0.2
+0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-2
N.D.
N.D.
-0.2
+0.2
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
N.D.
N.D.
+0.5
-0.2
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.2
-0.1
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
N.D.
+0.4
+0.2
-0.1
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
N.D.
N.D.
+0.2
+0.4
-0.0
phage
P. putida compost MOI 0.1
N.D.
N.D.
+0.3
+0.2
+0.2
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
N.D.
N.D.
+0.4
+0.3
-0.0
stress
R2A with Fusaric 480 ug/mL
N.D.
N.D.
+0.4
+0.2
+0.1
reactor
24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
N.D.
N.D.
+0.4
+0.1
+0.1
carbon source
ferulate (C); with MOPS
N.D.
N.D.
+0.2
-0.0
+0.6
phage
P. putida Sci small MOI 0.1
N.D.
N.D.
+0.2
+0.3
+0.3
stress
R2A with Fusaric 480 ug/mL
N.D.
N.D.
+0.5
+0.2
+0.1
phage
P. put South MOI 10
N.D.
N.D.
+0.4
+0.3
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
N.D.
N.D.
+0.4
+0.3
+0.2
pH
pH 4
N.D.
N.D.
+0.2
+0.4
+0.2
phage
P. putida Sci small MOI 0.1
N.D.
N.D.
+0.4
+0.1
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
N.D.
N.D.
+0.5
+0.4
+0.3
phage
P. putida PUT 7 MOI 1
N.D.
N.D.
+0.7
+0.3
+0.2
phage
P. putida Missley MOI 0.1
N.D.
N.D.
+0.4
+0.5
+0.4
phage
P. putida Sci Big MOI 0.1
N.D.
N.D.
+0.6
+0.6
+0.3
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