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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2235 and PP_2236 overlap by 22 nucleotides
PP_2236 and PP_2237 overlap by 17 nucleotides
PP_2237 and PP_2238 are separated by 127 nucleotides
PP_2238 and PP_5504 are separated by 39 nucleotides
PP_2235: PP_2235 - conserved protein of unknown function, at 2,542,541 to 2,542,921
_2235
PP_2236: PP_2236 - putative hydrolase of the alpha/beta superfamily, at 2,542,900 to 2,543,670
_2236
PP_2237: PP_2237 - conserved protein of unknown function, at 2,543,654 to 2,544,772
_2237
PP_2238: PP_2238 - putative metallopeptidase, at 2,544,900 to 2,546,801
_2238
PP_5504: PP_5504 - conserved protein of unknown function, at 2,546,841 to 2,547,122
_5504
Group
Condition
PP
_2235
PP
_2236
PP
_2237
PP
_2238
PP
_5504
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.1
-0.3
-0.0
-0.5
-1.0
phage
PUT8 MOI 0.1
+0.3
-0.2
-0.3
-0.1
-1.4
phage
P. put PUT 10 MOI 1
-0.2
-0.2
-0.1
+0.2
-1.0
phage
P. put PUT 10 MOI 10
-0.1
-0.1
+0.0
+0.1
-1.2
nitrogen source
nitrogen source nitrite 1mM
-0.4
+0.0
+0.1
-0.2
-0.7
phage
P. put PUT 10 MOI 10
+0.1
-0.0
-0.0
+0.1
-1.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
-0.3
+0.1
+0.2
-0.8
nitrogen source
Urea (N)
+0.3
-0.1
-0.2
-0.5
-0.3
carbon source
Valerolactone (C)
+0.3
-0.0
-0.1
+0.2
-1.2
micoeukaryotes
Tetrahymena sp., Mixed culture-2
+0.3
-0.1
+0.1
-0.6
-0.4
carbon source
Octanoic (C)
-0.5
-0.0
+0.0
-0.5
+0.5
phage
PUT 8 MOI 1
+0.4
+0.2
-0.3
+0.1
-0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.3
-0.0
-0.1
-0.6
+0.8
phage
PUT8 MOI 0.1
+0.7
-0.1
-0.2
+0.1
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.1
+0.3
-0.0
+0.4
-0.9
pH
Growth at pH9 and (C) Trisodium citrate
+0.5
-0.1
-0.0
+0.5
-0.6
carbon source
D-Glucose (C)
-0.6
-0.1
+0.2
+0.2
+0.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.2
+0.4
+0.1
+0.4
-0.8
nitrogen source
nitrate (N)
+0.4
-0.3
+0.1
-0.1
+0.5
carbon source
ferulate (C); with MOPS
+0.6
+0.1
+0.3
-0.5
+0.3
pH
Growth at pH6 and (C) D-Glucose
+0.5
+0.3
-0.0
-0.3
+0.6
temperature shift
Temperature shift 30_to_25
+0.8
-0.0
+0.1
+0.3
-0.2
carbon source
D-Glucose (C)
+0.4
+0.1
-0.2
-0.0
+0.9
nitrogen source
Urea (N)
-0.0
+0.2
-0.1
+0.5
+0.7
temperature shift
Temperature shift 30_to_25; with MOPS
+0.7
+0.2
+0.0
-0.2
+0.5
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.4
-0.1
-0.1
+0.2
+1.0
nitrogen source
NAG (N); with MOPS
+0.5
+0.3
+0.2
+0.1
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.2
+0.4
+0.3
+0.6
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.2
+0.2
-0.1
+1.4
phage
P. putida Missley MOI 0.1
+0.4
+0.1
+0.4
+0.7
+0.8
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