Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2222 and PP_2223 are separated by 285 nucleotidesPP_2223 and PP_2224 overlap by 4 nucleotidesPP_2224 and PP_2225 are separated by 12 nucleotidesPP_2225 and PP_2226 overlap by 4 nucleotides PP_2222: PP_2222 - conserved protein of unknown function, at 2,531,746 to 2,531,943 _2222 PP_2223: PP_2223 - conserved protein of unknown function, at 2,532,229 to 2,532,759 _2223 PP_2224: PP_2224 - conserved protein of unknown function, at 2,532,756 to 2,532,968 _2224 PP_2225: PP_2225 - putative Multicomponent potassium-proton antiporter, subunit G, at 2,532,981 to 2,533,346 _2225 PP_2226: PP_2226 - putative K(+)/H(+) antiporter subunit F, at 2,533,343 to 2,533,621 _2226
Group Condition PP_2222 PP_2223 PP_2224 PP_2225 PP_2226
nitrogen source Propandiamine (N) -1.1 -1.2 N.D. -0.2 -1.0
micoeukaryotes Dictyostellum sp.mixed culture-2 -1.1 -0.9 N.D. -0.3 -0.7
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X -0.4 -0.2 N.D. -0.6 -1.3
micoeukaryotes C.elegans, mixed culture-1 -0.7 -1.3 N.D. -0.3 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.2 N.D. -0.7 -0.8
micoeukaryotes Acanthamoeba sp., muxed culture-2 -1.1 -1.1 N.D. +0.1 -0.3
carbon source Vanillin (C) -0.8 -0.8 N.D. -0.2 -0.6
carbon source Trisodium citrate (C) -0.2 +0.2 N.D. -1.6 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 -0.4 N.D. -0.4 -1.0
nitrogen source spermidine (N) +0.1 +0.0 N.D. -1.0 -1.3
pH Growth at pH6 and (C) D-Glucose -1.0 +0.2 N.D. -0.3 -0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.7 +0.1 N.D. +0.1 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.4 -0.0 N.D. -0.7 +0.3
pH Growth at pH7 and (C) Trisodium citrate -0.5 -0.4 N.D. +0.3 -1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.1 -0.3 N.D. +0.4 +0.6
stress Growth with Chloride 500 mM -1.2 +0.2 N.D. +0.3 +0.7
carbon source ferulate (C); with MOPS +0.5 +0.7 N.D. +0.4 -1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.2 N.D. +0.9 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 -0.8 N.D. +0.6 +0.6
phage P. putida PUT10 MOI 10 +1.1 +1.1 N.D. -0.3 +0.2
phage P. putida PUT10 MOI 10 +0.6 +1.0 N.D. +0.2 +0.4
phage PUT8 MOI 0.1 +0.8 +1.2 N.D. +0.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.7 +0.0 N.D. +0.8 +0.9
stress Butanol 0.6 vol% +0.8 +1.0 N.D. +0.2 +0.4
carbon source Delta-Nonalactone (C) +0.6 +0.9 N.D. +0.1 +1.0
phage P. putida South MOI 10 +1.3 +1.5 N.D. +0.1 -0.1
phage P. put South MOI 10 +0.4 +1.5 N.D. +0.7 +0.2
phage P. putida South MOI 10 +1.2 +1.5 N.D. +0.2 +0.6
stress 1-Pentanol 0.3 vol% +0.9 +1.8 N.D. +0.4 +0.7
stress 3-methyl-1-butanol 0.4 vol% +1.6 +2.2 N.D. +0.5 +0.5
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