Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2182 and PP_2183 are separated by 401 nucleotidesPP_2183 and PP_2184 overlap by 25 nucleotidesPP_2184 and PP_2185 overlap by 4 nucleotidesPP_2185 and PP_2186 overlap by 4 nucleotides PP_2182: PP_2182 - conserved protein of unknown function, at 2,487,629 to 2,488,144 _2182 PP_2183: PP_2183 - putative Formate dehydrogenase, gamma subunit, at 2,488,546 to 2,489,028 _2183 PP_2184: PP_2184 - putative Formate dehydrogenase, beta subunit, at 2,489,004 to 2,490,581 _2184 PP_2185: PP_2185 - putative Formate dehydrogenase, alpha subunit, at 2,490,578 to 2,493,460 _2185 PP_2186: PP_2186 - putative Formate dehydrogenase, delta subunit, at 2,493,457 to 2,493,660 _2186
Group Condition PP_2182 PP_2183 PP_2184 PP_2185 PP_2186
phage P. putida Sci Big MOI 0.1 N.D. -0.4 -0.7 -0.4 -2.4
phage P. putida Sci small MOI 0.1 N.D. -1.1 -0.0 -0.2 -2.4
carbon source L-Phenylalanine 5 mM (C) N.D. -0.1 -0.1 -0.0 -1.6
phage PUT 8 MOI 1 N.D. -0.0 +0.0 -0.0 -1.6
nitrogen source nitrogen source nitrite 0.5mM N.D. -0.2 -0.1 -0.1 -1.0
nitrogen source 2,6-diaminopimelic (N) N.D. -0.7 -0.0 -0.2 -0.5
pH Growth at pH9 and (C) Trisodium citrate N.D. -0.3 -0.2 -0.3 -0.6
carbon source Delta-Dodecalactone (C) N.D. -0.4 +0.0 +0.1 -1.1
micoeukaryotes Dictyostellum sp.mixed culture-1 N.D. -0.4 +0.1 -0.0 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. +0.1 +0.0 -0.2 -1.1
micoeukaryotes Tetrahymena sp., Mixed culture-2 N.D. -0.6 -0.2 +0.1 -0.5
carbon source Tween 20 (C) N.D. +0.0 +0.1 -0.1 -1.1
pH Growth at pH6 and (C) Trisodium citrate N.D. -0.1 +0.1 +0.1 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.2 -0.1 +0.2 -0.9
carbon source D-Glucose (C) N.D. +0.3 -0.3 -0.2 -0.7
carbon source pyruvate (C) N.D. +0.4 +0.1 -0.0 -1.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. N.D. +0.4 -0.0 +0.1 -1.1
stress Cholinium alpha ketoglutarate 10 vol% N.D. +0.5 +0.0 +0.1 -0.8
carbon source carbon source 3-O-methyl-glucose 50 mM N.D. -0.7 -0.1 -0.1 +0.7
temperature Growth at 30C N.D. -0.4 -0.1 -0.1 +0.8
temperature shift Temperature shift 30_to_21; with MOPS N.D. -0.5 -0.3 +0.0 +1.0
carbon source carbon source 4-vinylphenol 0.1 mM N.D. -0.2 -0.1 -0.3 +0.9
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.5 +0.1 +0.4 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.1 -0.2 -0.2 +1.2
stress Cholinium glutamate 10 vol% N.D. -0.5 -0.0 +0.1 +1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs N.D. +0.3 -0.1 -0.3 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 +0.2 +0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 +0.1 -0.4 +1.0
carbon source carbon source Glycerol 108.6 mM N.D. +0.1 +0.1 -0.1 +1.4
nitrogen source D-2-Aminobutyric (N) N.D. +1.0 +0.3 +0.2 +0.3
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