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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2160 and PP_2161 are separated by 31 nucleotides
PP_2161 and PP_2162 are separated by 122 nucleotides
PP_2162 and PP_2163 are separated by 201 nucleotides
PP_2163 and PP_2164 are separated by 34 nucleotides
PP_2160: PP_2160 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 2,467,111 to 2,467,941
_2160
PP_2161: PP_2161 - conserved protein of unknown function, at 2,467,973 to 2,468,236
_2161
PP_2162: PP_2162 - putative phosphonoacetaldehyde phosphonohydrolase-related protein, at 2,468,359 to 2,468,922
_2162
PP_2163: PP_2163 - VacJ lipoprotein, at 2,469,124 to 2,469,831
_2163
PP_2164: PP_2164 - conserved protein of unknown function, at 2,469,866 to 2,470,165
_2164
Group
Condition
PP
_2160
PP
_2161
PP
_2162
PP
_2163
PP
_2164
stress
Geraniol 1 vol%
-1.6
N.D.
N.D.
-4.9
N.D.
carbon source
2,3-Butanediol (C)
-1.5
N.D.
N.D.
-4.5
N.D.
carbon source
Delta-Decalactone (C)
-1.7
N.D.
N.D.
-4.0
N.D.
stress
R2A with Fusaric 480 ug/mL
-0.2
N.D.
N.D.
-5.4
N.D.
stress
MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
-0.3
N.D.
N.D.
-5.0
N.D.
stress
2-Ethylhexanol 0.4 vol%
-0.2
N.D.
N.D.
-4.9
N.D.
stress
MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
-0.2
N.D.
N.D.
-4.9
N.D.
carbon source
Delta-Undecalactone (C)
-2.1
N.D.
N.D.
-2.4
N.D.
stress
pinene 10 vol%
-1.4
N.D.
N.D.
-3.1
N.D.
nitrogen source
Ammonium chloride and TNT (N)
+0.2
N.D.
N.D.
-4.7
N.D.
stress
R2A with Fusaric 480 ug/mL
+0.1
N.D.
N.D.
-4.5
N.D.
carbon source
ferulate (C); with MOPS
+0.1
N.D.
N.D.
-4.4
N.D.
carbon source
Delta-Decalactone (C)
-0.9
N.D.
N.D.
-3.3
N.D.
nitrogen source
Ammonium chloride and TNT (N)
+0.1
N.D.
N.D.
-4.3
N.D.
pH
Growth at pH6 and (C) D-Glucose
-2.6
N.D.
N.D.
-1.4
N.D.
stress
R2A with Fusaric 480 ug/mL
-0.9
N.D.
N.D.
-3.1
N.D.
carbon source
ferulate (C); with MOPS
+0.4
N.D.
N.D.
-4.4
N.D.
phage
P. putida South MOI 10
+0.3
N.D.
N.D.
-4.2
N.D.
nitrogen source
Ammonium chloride and TNT (N)
-0.4
N.D.
N.D.
-3.5
N.D.
carbon source
Ferulic Acid (C)
-0.5
N.D.
N.D.
-3.4
N.D.
stress
R2A with Fusaric 480 ug/mL
+0.2
N.D.
N.D.
-4.0
N.D.
carbon source
ferulate (C); with MOPS
+0.4
N.D.
N.D.
-3.6
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-3.6
N.D.
N.D.
+0.4
N.D.
carbon source
Decanoic (C)
+0.5
N.D.
N.D.
-3.5
N.D.
phage
P. putida South MOI 10
+1.1
N.D.
N.D.
-3.9
N.D.
phage
P. put South MOI 10
+0.6
N.D.
N.D.
-3.4
N.D.
phage
P. put PUT 10 MOI 0.1
+1.0
N.D.
N.D.
-3.2
N.D.
phage
P. putida South MOI 0.1
+1.6
N.D.
N.D.
-2.8
N.D.
phage
P. putida South MOI 0.1
+1.7
N.D.
N.D.
-2.4
N.D.
phage
P. putida South MOI 1
+1.9
N.D.
N.D.
-2.5
N.D.
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