Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_5503 and PP_2128 are separated by 348 nucleotidesPP_2128 and PP_2129 are separated by 55 nucleotidesPP_2129 and PP_2130 are separated by 112 nucleotidesPP_2130 and PP_2131 are separated by 172 nucleotides PP_5503: PP_5503 - putative CheA signal transduction histidine kinase, at 2,427,215 to 2,427,727 _5503 PP_2128: PP_2128 - putative CheV-like chemotaxis protein, at 2,428,076 to 2,429,011 _2128 PP_2129: PP_2129 - conserved protein of unknown function, at 2,429,067 to 2,429,870 _2129 PP_2130: PP_2130 - putative Soluble lytic transglycosylase, at 2,429,983 to 2,431,956 _2130 PP_2131: PP_2131 - DNA-binding ATPase involved in replication, at 2,432,129 to 2,434,057 _2131
Group Condition PP_5503 PP_2128 PP_2129 PP_2130 PP_2131
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.1 -1.0 -0.2 -2.3
phage P. putida PUT 7 MOI 1 -0.5 +0.1 -0.4 -1.0 -1.3
nitrogen source 2,6-diaminopimelic (N) +0.4 -0.3 -2.5 -0.3 -0.0
phage P. putida Sci Big MOI 0.1 -0.2 -0.1 -1.9 +0.1 -0.1
nitrogen source D-2-Aminobutyric (N) +0.1 +0.3 -0.8 -0.8 -1.0
phage P. putida Missley MOI 0.1 -0.5 -0.2 -0.4 -0.2 -0.9
carbon source Decanoic (C) -0.1 -0.2 -0.3 -1.4 -0.1
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -0.0 -0.4 -0.9 -0.1 -0.6
stress R2A with Fusaric 480 ug/mL -0.3 -0.1 -0.2 -0.9 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.3 -0.3 -1.1 -0.2 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.0 -0.1 -0.5 -0.3 -1.0
carbon source D-Glucose (C); with MOPS -0.4 +0.1 -0.5 -0.2 -0.8
stress R2A with Fusaric 480 ug/mL -0.1 +0.1 -0.4 -1.3 -0.2
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL +0.0 -0.2 +0.1 -1.3 -0.4
carbon source carbon source 3-O-methyl-glucose 50 mM -0.2 +0.1 -1.2 +0.1 -0.5
stress R2A with Fusaric 480 ug/mL -0.1 +0.2 -0.4 -1.6 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.0 -0.1 +0.3 +0.3 -2.2
carbon source carbon source 3-O-methyl-glucose 10 mM +0.2 -0.3 -0.8 +0.2 -0.9
carbon source ferulate (C); with MOPS -0.1 +0.2 -0.6 -0.9 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.2 +0.2 -0.1 -0.3 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 -0.2 +0.5 -0.3 -0.7
carbon source 3-Hydroxydecanoic (C) -0.0 -0.3 +0.5 -0.3 -0.9
carbon source Delta-Decalactone (C) +0.1 +0.5 -0.3 -0.7 -0.4
phage P. putida Sci small MOI 0.1 +0.3 -0.0 +0.4 -0.6 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.0 +0.3 +0.5 -0.8 -0.7
pH Growth at pH9 and (C) Trisodium citrate +0.2 +0.1 -1.0 -0.3 +0.5
carbon source carbon source Bisabolol 45 mM +0.1 -0.2 -0.5 +0.7 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 -0.3 -0.1 -0.7 +0.6
carbon source 4-Aminobutyric 10 mM (C) +0.2 -0.4 -0.8 +0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.1 +1.0 -0.5 -0.3
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