Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2084 and PP_2085 are separated by 24 nucleotidesPP_2085 and PP_2086 are separated by 87 nucleotidesPP_2086 and PP_2087 are separated by 2 nucleotidesPP_2087 and PP_2088 are separated by 89 nucleotides PP_2084: PP_2084 - Putative regulator of ribonuclease activity, at 2,376,844 to 2,377,335 _2084 PP_2085: PP_2085 - CmaX protein, at 2,377,360 to 2,378,358 _2085 PP_2086: PP_2086 - conserved protein of unknown function, CrfX family, at 2,378,446 to 2,378,718 _2086 PP_2087: PP_2087 - CmpX protein, at 2,378,721 to 2,379,545 _2087 PP_2088: PP_2088 - RNA polymerase sigma factor SigX, at 2,379,635 to 2,380,225 _2088
Group Condition PP_2084 PP_2085 PP_2086 PP_2087 PP_2088
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB +0.1 N.D. N.D. -3.0 -5.2
nitrogen source nitrogen source nitrate 5mM +0.2 N.D. N.D. -1.3 -6.9
nitrogen source nitrogen source nitrate 20mM +0.2 N.D. N.D. -0.6 -7.1
nitrogen source L-Lysine (N) -0.2 N.D. N.D. -0.4 -6.7
nitrogen source D-2-Aminobutyric (N) +0.0 N.D. N.D. -2.7 -4.6
nitrogen source Propiolactam (N) +0.3 N.D. N.D. -1.6 -5.9
pH Growth at pH6 and (C) Trisodium citrate +0.0 N.D. N.D. -1.7 -5.3
nitrogen source D-Lysine (N) -0.0 N.D. N.D. -0.7 -6.2
nitrogen source Propiolactam (N) -0.2 N.D. N.D. -1.2 -5.4
nitrogen source L-Isoleucine (N) -0.1 N.D. N.D. -0.5 -6.1
nitrogen source 3-aminobutyric (N) -0.0 N.D. N.D. -0.9 -5.7
carbon source D-Lysine 10 mM (C) -0.0 N.D. N.D. -2.2 -4.4
nitrogen source D-Lysine (N) -0.3 N.D. N.D. +0.0 -6.2
nitrogen source L-Threonine (N) -0.2 N.D. N.D. -0.9 -5.3
pH Growth at pH7 and (C) D-Glucose -0.4 N.D. N.D. -5.9 -0.1
carbon source Soil Extract with D-Glucose 10 mM; 30C; then outgrowth in LB +0.1 N.D. N.D. -1.3 -5.1
temperature Growth at 30C; with MOPS -0.2 N.D. N.D. -0.8 -5.2
nitrogen source DL-2-Aminobutyric (N) +0.0 N.D. N.D. -0.2 -6.1
nitrogen source nitrogen source nitrate 20mM -0.0 N.D. N.D. -1.6 -4.6
stress R2A with Fusaric 480 ug/mL -0.4 N.D. N.D. -0.7 -5.0
temperature shift Temperature shift 30_to_25 -0.5 N.D. N.D. -0.6 -5.0
nitrogen source L-Isoleucine (N) -0.0 N.D. N.D. -0.7 -5.3
nitrogen source L-Lysine (N) -0.2 N.D. N.D. -1.5 -4.3
carbon source 2-Methylbutanoic (C) -1.8 N.D. N.D. -3.4 -0.7
carbon source D-Lysine 10 mM (C) -0.2 N.D. N.D. -1.8 -3.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.1 N.D. N.D. -3.6 -2.1
carbon source Soil Extract with Trisodium citrate 10 mM; 30C -0.1 N.D. N.D. -0.6 -4.9
nitrogen source nitrate (N) -0.0 N.D. N.D. -0.5 -5.1
nitrogen source NAG (N) +0.2 N.D. N.D. -0.4 -5.3
pH Growth at pH9 and (C) Trisodium citrate +0.1 N.D. N.D. -0.5 -5.2
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