Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2074 and PP_2075 are separated by 56 nucleotidesPP_2075 and PP_2076 overlap by 4 nucleotidesPP_2076 and PP_2077 are separated by 332 nucleotidesPP_2077 and PP_2078 are separated by 123 nucleotides PP_2074: PP_2074 - Transcriptional regulator, LysR family, at 2,359,043 to 2,359,966 _2074 PP_2075: PP_2075 - conserved membrane protein of unknown function, at 2,360,023 to 2,361,156 _2075 PP_2076: PP_2076 - conserved protein of unknown function, at 2,361,153 to 2,363,876 _2076 PP_2077: PP_2077 - diaminopimelate decarboxylase, at 2,364,209 to 2,365,441 _2077 PP_2078: PP_2078 - transcriptional activator protein LysR, at 2,365,565 to 2,366,470 _2078
Group Condition PP_2074 PP_2075 PP_2076 PP_2077 PP_2078
phage P. putida Sci small MOI 0.1 -1.9 +0.6 +0.2 -0.5 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.6 -1.2 -0.0 -1.1 +0.7
pH Growth at pH7 and (C) Trisodium citrate -0.3 -0.4 -0.8 -0.6 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 -0.0 -0.1 -1.2 -0.3
nitrogen source 2,6-diaminopimelic (N) -0.7 -0.5 -0.6 +0.5 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.2 +0.1 -0.2 -1.0 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.8 +0.2 -0.3 -0.7 -0.2
stress R2A with Fusaric 240 ug/mL -0.5 +0.3 -0.2 -0.8 -0.7
pH Growth at pH7 and (C) D-Glucose -1.8 +0.2 +0.1 -0.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.2 +0.2 +0.1 -0.9 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.8 -0.3 -0.0 -0.9 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.6 -0.1 -0.5 -0.5 -0.9
carbon source D-Glucose (C) -0.4 -0.5 -0.2 +0.4 -0.6
biofilm mediium pdms biofilm mediium PDMS; 5.5 hours -0.6 +0.2 +0.5 -0.4 -0.6
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.0 -0.2 -0.2 +0.7 -1.1
nitrogen source nitrogen source nitrite 1mM -0.0 -0.7 -0.3 +0.8 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 -0.0 -0.4 -1.1 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs -0.6 +0.2 -0.2 -0.6 +0.6
attachment on pdms attachment on PDMS; 2 hours -0.3 +0.3 +0.4 +0.4 -1.2
temperature Growth at 30C; with MOPS +0.6 +0.3 +0.1 -0.5 -0.8
temperature Growth at 25C; with MOPS -0.1 -0.7 +0.4 +0.6 -0.4
stress Growth with Chloride 500 mM; with MOPS +0.3 -0.5 -0.3 +0.9 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 +0.7 +0.6 -0.2 -0.5
pH Valerolactone (C) pH 6.5 -0.2 +0.6 +0.1 +0.6 -0.9
phage PUT 8 MOI 1 +0.3 -0.2 +0.4 +0.5 -0.9
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.5 +0.3 -0.4 -0.6 +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +0.8 -0.1 -0.6 +0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.9 -0.1 -0.7 +0.2 +0.8
pH Growth at pH8 and (C) D-Glucose +0.4 +0.5 -0.7 +0.7 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 +0.4 +0.5 -0.3 -0.2
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