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Fitness for 5 genes in
Pseudomonas putida KT2440
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PP_2070 and PP_2071 are separated by 169 nucleotides
PP_2071 and PP_2072 are separated by 108 nucleotides
PP_2072 and PP_2073 are separated by 19 nucleotides
PP_2073 and PP_2074 are separated by 197 nucleotides
PP_2070: PP_2070 - Transcriptional regulator, AraC family, at 2,356,111 to 2,356,821
_2070
PP_2071: PP_2071 - conserved protein of unknown function, at 2,356,991 to 2,357,377
_2071
PP_2072: PP_2072 - Transcriptional regulator, AraC family, at 2,357,486 to 2,358,310
_2072
PP_2073: PP_2073 - Acetyltransferase, GNAT family, at 2,358,330 to 2,358,845
_2073
PP_2074: PP_2074 - Transcriptional regulator, LysR family, at 2,359,043 to 2,359,966
_2074
Group
Condition
PP
_2070
PP
_2071
PP
_2072
PP
_2073
PP
_2074
phage
P. putida Sci small MOI 0.1
-4.4
-2.6
-0.1
+1.2
-1.9
phage
P. putida Sci Big MOI 0.1
-1.6
-0.5
-0.1
-2.1
-0.1
pH
Growth at pH7 and (C) D-Glucose
-1.6
+0.5
-0.5
-0.2
-1.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-2.4
+0.1
+0.4
-2.5
+0.9
pH
Growth at pH8 and (C) D-Glucose
-2.4
-0.2
+0.2
-1.3
+0.4
carbon source
L-Phenylalanine 5 mM (C)
-2.4
-0.4
-0.2
-0.2
-0.1
carbon source
Trisodium citrate (C)
-0.9
-0.4
-0.4
-1.4
-0.1
carbon source
D-Glucose (C)
-1.7
-0.4
-0.2
-0.9
+0.2
nitrogen source
2,6-diaminopimelic (N)
-1.3
-0.7
+0.0
-0.2
-0.7
nitrogen source
nitrite (N)
-1.1
-0.2
+0.2
-1.2
-0.4
nitrogen source
no extra (N); with MOPS
-2.4
+0.1
-0.1
-0.6
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-1.5
+0.2
+0.1
-1.1
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.4
-0.3
+0.6
-0.9
-1.6
carbon source
ferulate (C); with MOPS
-1.9
+0.5
-0.3
+0.4
-1.0
pH
Growth at pH6 and (C) Trisodium citrate
+0.7
-0.3
-0.1
-2.1
-0.0
carbon source
carbon source D-Glucose 55.5 mM
-0.1
+0.0
+0.3
-2.4
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.8
+0.3
+0.0
-2.3
-0.6
pH
Growth at pH8 and (C) D-Glucose
-2.2
+0.1
-0.1
+0.5
+0.1
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-2.2
+0.4
+0.1
+0.6
-0.2
pH
Growth at pH6 and (C) Trisodium citrate
-1.7
-0.3
+0.3
+0.7
-0.3
temperature shift
Temperature shift 30_to_25; with MOPS
-1.7
-0.2
-0.3
+0.4
+0.6
stress
R2A with Polymyxin B sulfate 0.001 mg/ml
-1.1
+0.7
+0.2
-1.1
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+1.0
+0.4
+0.4
-1.4
-1.1
phage
P. putida South MOI 10
-1.7
+0.3
-0.2
+0.7
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.7
-0.4
-0.2
-1.0
+0.6
phage
PUT 8 MOI 1
+1.6
-0.2
+0.0
-1.5
+0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
+1.3
+0.3
+0.4
-0.2
-1.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.6
+0.1
-0.1
-0.3
+0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+2.1
+0.4
+0.3
+0.5
-1.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.3
+0.2
+0.2
+1.1
-0.5
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