Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2070 and PP_2071 are separated by 169 nucleotidesPP_2071 and PP_2072 are separated by 108 nucleotidesPP_2072 and PP_2073 are separated by 19 nucleotidesPP_2073 and PP_2074 are separated by 197 nucleotides PP_2070: PP_2070 - Transcriptional regulator, AraC family, at 2,356,111 to 2,356,821 _2070 PP_2071: PP_2071 - conserved protein of unknown function, at 2,356,991 to 2,357,377 _2071 PP_2072: PP_2072 - Transcriptional regulator, AraC family, at 2,357,486 to 2,358,310 _2072 PP_2073: PP_2073 - Acetyltransferase, GNAT family, at 2,358,330 to 2,358,845 _2073 PP_2074: PP_2074 - Transcriptional regulator, LysR family, at 2,359,043 to 2,359,966 _2074
Group Condition PP_2070 PP_2071 PP_2072 PP_2073 PP_2074
phage P. putida Sci small MOI 0.1 -4.4 -2.6 -0.1 +1.2 -1.9
phage P. putida Sci Big MOI 0.1 -1.6 -0.5 -0.1 -2.1 -0.1
pH Growth at pH7 and (C) D-Glucose -1.6 +0.5 -0.5 -0.2 -1.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.4 +0.1 +0.4 -2.5 +0.9
pH Growth at pH8 and (C) D-Glucose -2.4 -0.2 +0.2 -1.3 +0.4
carbon source L-Phenylalanine 5 mM (C) -2.4 -0.4 -0.2 -0.2 -0.1
carbon source Trisodium citrate (C) -0.9 -0.4 -0.4 -1.4 -0.1
carbon source D-Glucose (C) -1.7 -0.4 -0.2 -0.9 +0.2
nitrogen source 2,6-diaminopimelic (N) -1.3 -0.7 +0.0 -0.2 -0.7
nitrogen source nitrite (N) -1.1 -0.2 +0.2 -1.2 -0.4
nitrogen source no extra (N); with MOPS -2.4 +0.1 -0.1 -0.6 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -1.5 +0.2 +0.1 -1.1 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 -0.3 +0.6 -0.9 -1.6
carbon source ferulate (C); with MOPS -1.9 +0.5 -0.3 +0.4 -1.0
pH Growth at pH6 and (C) Trisodium citrate +0.7 -0.3 -0.1 -2.1 -0.0
carbon source carbon source D-Glucose 55.5 mM -0.1 +0.0 +0.3 -2.4 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.8 +0.3 +0.0 -2.3 -0.6
pH Growth at pH8 and (C) D-Glucose -2.2 +0.1 -0.1 +0.5 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.2 +0.4 +0.1 +0.6 -0.2
pH Growth at pH6 and (C) Trisodium citrate -1.7 -0.3 +0.3 +0.7 -0.3
temperature shift Temperature shift 30_to_25; with MOPS -1.7 -0.2 -0.3 +0.4 +0.6
stress R2A with Polymyxin B sulfate 0.001 mg/ml -1.1 +0.7 +0.2 -1.1 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +1.0 +0.4 +0.4 -1.4 -1.1
phage P. putida South MOI 10 -1.7 +0.3 -0.2 +0.7 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 -0.4 -0.2 -1.0 +0.6
phage PUT 8 MOI 1 +1.6 -0.2 +0.0 -1.5 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +1.3 +0.3 +0.4 -0.2 -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.6 +0.1 -0.1 -0.3 +0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +2.1 +0.4 +0.3 +0.5 -1.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 +0.2 +0.2 +1.1 -0.5
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