Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2067 and PP_2068 overlap by 11 nucleotidesPP_2068 and PP_2069 are separated by 57 nucleotidesPP_2069 and PP_2070 are separated by 100 nucleotidesPP_2070 and PP_2071 are separated by 169 nucleotides PP_2067: PP_2067 - putative EmrB/QacA family drug resistance transporter, at 2,351,750 to 2,353,264 _2067 PP_2068: PP_2068 - putative Multidrug efflux MFS membrane fusion protein, at 2,353,254 to 2,354,531 _2068 PP_2069: PP_2069 - putative Multidrug efflux MFS outer membrane protein, at 2,354,589 to 2,356,010 _2069 PP_2070: PP_2070 - Transcriptional regulator, AraC family, at 2,356,111 to 2,356,821 _2070 PP_2071: PP_2071 - conserved protein of unknown function, at 2,356,991 to 2,357,377 _2071
Group Condition PP_2067 PP_2068 PP_2069 PP_2070 PP_2071
phage P. putida Sci small MOI 0.1 -0.2 -0.4 +0.4 -4.4 -2.6
phage P. putida Sci Big MOI 0.1 -0.6 -1.3 -0.8 -1.6 -0.5
carbon source L-Phenylalanine 5 mM (C) -0.5 -0.2 -0.0 -2.4 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.8 -0.1 +0.1 -2.4 +0.1
pH pH 7 -0.5 -0.0 +0.1 -2.1 -0.4
nitrogen source no extra (N); with MOPS -0.2 -0.2 -0.1 -2.4 +0.1
pH Growth at pH8 and (C) D-Glucose -0.1 +0.3 -0.2 -2.4 -0.2
carbon source D-Glucose (C) -0.6 -0.1 -0.1 -0.9 -0.7
pH Growth at pH6 and (C) Trisodium citrate -0.2 -0.1 -0.1 -1.7 -0.3
pH Growth at pH8 and (C) D-Glucose -0.1 -0.2 +0.0 -2.2 +0.1
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM +0.1 -0.4 +0.2 -1.8 -0.1
carbon source D-Glucose (C) +0.3 -0.1 -0.1 -1.7 -0.4
phage P. putida South MOI 10 -0.2 -0.4 +0.0 -1.7 +0.3
pH Growth at pH6 and (C) D-Glucose -0.4 -0.2 -0.1 -1.5 +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.1 +0.1 -2.2 +0.4
temperature shift Temperature shift 30_to_25 +0.5 -0.2 -0.3 -1.2 -0.5
nitrogen source NAG (N); with MOPS -1.1 +0.1 +0.3 -0.2 -0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.1 -0.1 -1.0 +0.4
carbon source ferulate (C); with MOPS +0.1 -0.3 +0.3 -1.9 +0.5
temperature shift Temperature shift 30_to_25; with MOPS +0.7 +0.0 +0.1 -1.7 -0.2
pH Growth at pH7 and (C) D-Glucose +0.2 -0.0 -0.1 -1.6 +0.5
stress R2A with Polymyxin B sulfate 0.001 mg/ml -0.4 +0.0 -0.1 -1.1 +0.7
temperature Growth at 25C +0.3 +0.0 +0.1 -1.3 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.2 +0.3 +0.1 -0.2 +0.8
temperature shift Temperature shift 10_to_25; with MOPS +0.7 +0.2 +0.2 -1.1 +0.6
micoeukaryotes Tetrahymena sp., Mixed culture-2 -0.6 +0.5 +0.2 -0.2 +1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.4 +0.1 -0.2 +1.4 -0.3
phage PUT 8 MOI 1 +0.4 +0.3 +0.1 +1.6 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.0 +0.0 -0.3 +2.1 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.7 +0.0 -0.1 +1.3 +0.3
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