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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_2050 and PP_2051 overlap by 4 nucleotides
PP_2051 and PP_2052 are separated by 79 nucleotides
PP_2052 and PP_5500 are separated by 79 nucleotides
PP_5500 and PP_2053 are separated by 113 nucleotides
PP_2050: PP_2050 - conserved protein of unknown function, at 2,332,620 to 2,333,072
_2050
PP_2051: PP_2051 - 3-ketoacyl-CoA thiolase (thiolase I), at 2,333,069 to 2,334,253
_2051
PP_2052: PP_2052 - putative bifunctional enzyme: sugar-phosphatase/mannitol-1-phosphate 5-dehydrogenase, at 2,334,333 to 2,336,456
_2052
PP_5500: PP_5500 - protein of unknown function, at 2,336,536 to 2,336,739
_5500
PP_2053: PP_2053 - conserved protein of unknown function, at 2,336,853 to 2,337,149
_2053
Group
Condition
PP
_2050
PP
_2051
PP
_2052
PP
_5500
PP
_2053
stress
Growth with Chloride 500 mM; with MOPS
-0.3
+0.4
-2.3
-4.3
-0.0
stress
Growth with Chloride 500 mM
+0.2
-0.1
-2.3
-3.7
-0.2
stress
Growth with Chloride 500 mM
+0.1
-0.2
-2.5
-3.0
-0.2
phage
P. putida Missley MOI 0.1
-1.0
-0.1
-0.5
-3.0
-0.9
stress
Growth with Chloride 500 mM; with MOPS
+0.2
+0.0
-2.7
-2.7
-0.0
stress
Growth with Chloride 500 mM; with MOPS
-0.1
-0.3
-2.4
-2.1
-0.0
stress
Growth with Chloride 500 mM; with MOPS
+0.0
+0.1
-2.5
-2.4
+0.3
phage
PUT 8 MOI 1
-0.1
-0.3
-0.1
-3.7
+0.0
stress
Growth with Chloride 500 mM; with MOPS
-0.1
+0.1
-2.5
-1.8
+0.1
stress
Growth with Chloride 500 mM
+0.1
-0.2
-1.9
-1.8
-0.1
phage
P. putida PUT 7 MOI 1
-0.9
-0.9
-0.9
-0.3
-0.7
stress
Growth with Chloride 500 mM; with MOPS
+0.2
-0.0
-2.3
-1.3
-0.1
nitrogen source
2,6-diaminopimelic (N)
-0.6
-0.5
+0.1
-1.4
-0.3
nitrogen source
D-2-Aminobutyric (N)
-0.6
+0.3
-0.0
-2.0
-0.2
phage
P. putida Sci small MOI 0.1
-0.6
-0.1
-0.2
-1.5
-0.1
nitrogen source
D-2-Aminobutyric (N)
-0.3
-0.5
+0.0
-0.4
-1.3
nitrogen source
NAG (N); with MOPS
+0.0
-0.2
+0.4
-1.8
-0.8
carbon source
D-Glucose (C)
+0.2
+0.1
+0.0
-1.9
-0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.3
+0.4
-0.2
-2.3
-0.2
phage
P. putida Sci Big MOI 0.1
-0.7
-0.4
-0.4
+0.5
-0.8
temperature shift
Temperature shift 30_to_25; with MOPS
+0.2
+0.2
+0.1
-1.7
-0.1
nitrogen source
nitrite (N)
+0.4
-0.2
+0.2
-1.4
-0.2
carbon source
ferulate (C); with MOPS
+0.0
-0.3
+0.4
-1.8
+0.5
carbon source
Myristic (C)
-1.0
+0.5
-0.3
+0.4
-0.3
nitrogen source
NAG (N); with MOPS
+0.2
-0.2
+0.3
-1.4
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.1
+0.3
+0.3
+0.6
-1.2
temperature
Growth at 25C
-0.5
-0.4
+0.6
+0.5
+0.4
pH
Growth at pH7 and (C) Trisodium citrate
+0.2
+0.3
-0.5
+0.6
+0.8
carbon source
4-Aminobutyric 10 mM (C)
-0.3
-0.0
-0.0
+1.7
+0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.1
+0.3
+1.9
+0.1
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