Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_2007 and PP_2008 are separated by 281 nucleotidesPP_2008 and PP_2009 are separated by 236 nucleotidesPP_2009 and PP_2010 overlap by 10 nucleotidesPP_2010 and PP_2011 are separated by 228 nucleotides PP_2007: PP_2007 - P-47-related protein, at 2,277,347 to 2,278,579 _2007 PP_2008: PP_2008 - 2,4-dienoyl-CoA reductase, at 2,278,861 to 2,280,942 _2008 PP_2009: PP_2009 - putative 1-aminocyclopropane-1-carboxylate deaminase, at 2,281,179 to 2,282,072 _2009 PP_2010: PP_2010 - Cytochrome b561, at 2,282,063 to 2,282,608 _2010 PP_2011: PP_2011 - conserved exported protein of unknown function, at 2,282,837 to 2,283,814 _2011
Group Condition PP_2007 PP_2008 PP_2009 PP_2010 PP_2011
phage P. putida Sci small MOI 0.1 -0.2 -0.1 -1.6 -0.2 +0.1
nitrogen source 2,6-diaminopimelic (N) -0.1 -0.3 -0.3 -0.1 -0.9
phage P. putida PUT 7 MOI 1 -0.2 -0.1 -1.3 +0.0 -0.2
phage P. putida Sci small MOI 0.1 -0.2 -0.4 -0.9 +0.1 -0.2
stress R2A with Fusaric 240 ug/mL -0.1 -0.3 -0.4 -0.7 -0.1
phage P. put South MOI 10 -0.3 -0.4 -0.2 -0.3 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 -0.3 +0.2 +0.2 -0.9
carbon source Valerolactone (C) +0.0 -0.4 -0.6 +0.3 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.4 +0.2 -0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.1 +0.2 -0.1 +0.2 -0.7
pH Growth at pH7 and (C) D-Glucose -0.2 +0.3 -0.5 +0.2 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.5 -0.5 +0.4 -0.1
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. +0.1 +0.0 -0.5 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.4 -0.6 +0.3 -0.6
dilutelb LB 0.1x with 55.5 mM D-Glucose +0.3 +0.2 +0.2 -0.6 -0.4
micoeukaryotes Acanthamoeba sp., muxed culture-2 +0.3 -0.2 +0.2 -0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.3 -0.3 +0.3 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 -0.0 +0.5 -0.8 +0.2
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -0.3 -0.2 +0.3 -0.3 +0.3
stress 1-Pentanol 0.3 vol% +0.1 +0.2 -0.2 -0.5 +0.4
carbon source D-Glucose (C) -0.1 +0.2 -0.6 +0.0 +0.4
nitrogen source Propandiamine (N) +0.0 +0.1 +0.5 -0.7 +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 -0.5 +0.1 +0.6 -0.5
carbon source ferulate (C); with MOPS -0.3 +0.2 +0.3 +0.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.1 +0.1 +1.1 -0.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.3 +0.2 -0.2 +0.4 -0.4
pH pH 6 +0.0 -0.1 +0.8 -0.6 +0.4
temperature shift Temperature shift 30_to_25; with MOPS +0.0 +0.2 +0.6 +0.1 -0.4
pH Valerolactone (C) pH 6.5 +0.2 +0.3 +0.7 -0.1 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.2 +0.4 +0.2 +0.5
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