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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PP_2007 and PP_2008 are separated by 281 nucleotides
PP_2008 and PP_2009 are separated by 236 nucleotides
PP_2009 and PP_2010 overlap by 10 nucleotides
PP_2010 and PP_2011 are separated by 228 nucleotides
PP_2007: PP_2007 - P-47-related protein, at 2,277,347 to 2,278,579
_2007
PP_2008: PP_2008 - 2,4-dienoyl-CoA reductase, at 2,278,861 to 2,280,942
_2008
PP_2009: PP_2009 - putative 1-aminocyclopropane-1-carboxylate deaminase, at 2,281,179 to 2,282,072
_2009
PP_2010: PP_2010 - Cytochrome b561, at 2,282,063 to 2,282,608
_2010
PP_2011: PP_2011 - conserved exported protein of unknown function, at 2,282,837 to 2,283,814
_2011
Group
Condition
PP
_2007
PP
_2008
PP
_2009
PP
_2010
PP
_2011
phage
P. putida Sci small MOI 0.1
-0.2
-0.1
-1.6
-0.2
+0.1
nitrogen source
2,6-diaminopimelic (N)
-0.1
-0.3
-0.3
-0.1
-0.9
phage
P. putida PUT 7 MOI 1
-0.2
-0.1
-1.3
+0.0
-0.2
phage
P. putida Sci small MOI 0.1
-0.2
-0.4
-0.9
+0.1
-0.2
stress
R2A with Fusaric 240 ug/mL
-0.1
-0.3
-0.4
-0.7
-0.1
phage
P. put South MOI 10
-0.3
-0.4
-0.2
-0.3
+0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.1
-0.3
+0.2
+0.2
-0.9
carbon source
Valerolactone (C)
+0.0
-0.4
-0.6
+0.3
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.4
+0.2
-0.3
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-0.1
+0.2
-0.1
+0.2
-0.7
pH
Growth at pH7 and (C) D-Glucose
-0.2
+0.3
-0.5
+0.2
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.5
-0.5
+0.4
-0.1
reactor
40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
+0.1
+0.0
-0.5
+0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.4
-0.6
+0.3
-0.6
dilutelb
LB 0.1x with 55.5 mM D-Glucose
+0.3
+0.2
+0.2
-0.6
-0.4
micoeukaryotes
Acanthamoeba sp., muxed culture-2
+0.3
-0.2
+0.2
-0.3
-0.4
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.3
-0.3
+0.3
-0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.1
-0.0
+0.5
-0.8
+0.2
stress
MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
-0.3
-0.2
+0.3
-0.3
+0.3
stress
1-Pentanol 0.3 vol%
+0.1
+0.2
-0.2
-0.5
+0.4
carbon source
D-Glucose (C)
-0.1
+0.2
-0.6
+0.0
+0.4
nitrogen source
Propandiamine (N)
+0.0
+0.1
+0.5
-0.7
+0.1
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.5
+0.1
+0.6
-0.5
carbon source
ferulate (C); with MOPS
-0.3
+0.2
+0.3
+0.2
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
-0.1
+0.1
+1.1
-0.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.3
+0.2
-0.2
+0.4
-0.4
pH
pH 6
+0.0
-0.1
+0.8
-0.6
+0.4
temperature shift
Temperature shift 30_to_25; with MOPS
+0.0
+0.2
+0.6
+0.1
-0.4
pH
Valerolactone (C) pH 6.5
+0.2
+0.3
+0.7
-0.1
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
-0.2
+0.4
+0.2
+0.5
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