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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1961 and PP_1962 overlap by 1 nucleotides
PP_1962 and PP_1963 are separated by 82 nucleotides
PP_1963 and PP_1964 overlap by 35 nucleotides
PP_1964 and PP_1966 are separated by 446 nucleotides
PP_1961: PP_1961 - conserved protein of unknown function, at 2,221,629 to 2,222,618
_1961
PP_1962: PP_1962 - Site-specific recombinase, phage integrase family, at 2,222,618 to 2,224,546
_1962
PP_1963: PP_1963 - conserved protein of unknown function, at 2,224,629 to 2,226,632
_1963
PP_1964: PP_1964 - putative dGMP, dTMP, dhydroxyCMP kinase, at 2,226,598 to 2,227,401
_1964
PP_1966: PP_1966 - DNA polymerase III subunit delta', at 2,227,848 to 2,228,834
_1966
Group
Condition
PP
_1961
PP
_1962
PP
_1963
PP
_1964
PP
_1966
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.5
-0.5
-1.6
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.9
-0.2
-0.8
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.6
-0.3
-1.0
N.D.
N.D.
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.6
-0.5
-0.3
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.6
-0.4
-0.4
N.D.
N.D.
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs
-0.5
-0.3
-0.6
N.D.
N.D.
pH
Growth at pH6 and (C) D-Glucose
-0.6
+0.2
-1.0
N.D.
N.D.
carbon source
D-Glucose (C)
-0.4
-0.2
-0.7
N.D.
N.D.
micoeukaryotes
C.elegans, mixed culture-2
-0.5
+0.1
-0.7
N.D.
N.D.
nitrogen source
nitrate (N)
-0.7
+0.4
-0.4
N.D.
N.D.
pH
Valerolactone (C) pH 6.5
-0.2
-0.8
+0.4
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.4
-1.1
N.D.
N.D.
temperature
Growth at 25C
+0.6
+0.1
-1.0
N.D.
N.D.
nitrogen source
D-2-Aminobutyric (N)
+0.2
+0.7
-0.9
N.D.
N.D.
nitrogen source
no extra (N); with MOPS
+0.6
+0.1
-0.7
N.D.
N.D.
carbon source
acetate (C)
-0.4
+0.2
+0.7
N.D.
N.D.
phage
P. put South MOI 10
+0.9
+0.5
-0.2
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.6
+0.2
N.D.
N.D.
phage
P. putida South MOI 10
+0.6
+0.5
+0.2
N.D.
N.D.
nitrogen source
Urea (N); with MOPS
+0.5
+0.2
+0.8
N.D.
N.D.
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
+0.6
+0.2
+0.7
N.D.
N.D.
carbon source
carbon source 3-O-methyl-glucose 50 mM
+0.5
+0.2
+0.8
N.D.
N.D.
phage
P. putida Sci Big MOI 0.1
+0.4
+0.8
+0.4
N.D.
N.D.
phage
P. putida Sci small MOI 0.1
+0.7
+0.4
+0.7
N.D.
N.D.
phage
PUT8 MOI 10
+0.5
+0.3
+0.9
N.D.
N.D.
phage
P. putida PUT 7 MOI 1
+0.4
+1.0
+0.5
N.D.
N.D.
phage
PUT8 MOI 0.1
+0.5
+0.6
+0.9
N.D.
N.D.
phage
PUT8 MOI 0.1
+1.0
+0.5
+0.6
N.D.
N.D.
phage
P. putida Missley MOI 0.1
+0.4
+0.8
+1.0
N.D.
N.D.
phage
P. putida Sci small MOI 0.1
+1.0
+0.9
+0.6
N.D.
N.D.
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