Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1918 and PP_5482 are separated by 821 nucleotidesPP_5482 and PP_1920 are separated by 163 nucleotidesPP_1920 and PP_5483 are separated by 67 nucleotidesPP_5483 and PP_1921 are separated by 780 nucleotides PP_1918: PP_1918 - septation protein, at 2,161,053 to 2,162,318 _1918 PP_5482: PP_5482 - protein of unknown function, at 2,163,140 to 2,164,153 _5482 PP_1920: PP_1920 - conserved protein of unknown function, at 2,164,317 to 2,165,537 _1920 PP_5483: PP_5483 - conserved protein of unknown function, at 2,165,605 to 2,166,156 _5483 PP_1921: PP_1921 - conserved protein of unknown function, at 2,166,937 to 2,168,100 _1921
Group Condition PP_1918 PP_5482 PP_1920 PP_5483 PP_1921
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.4 -0.3 -0.4 -0.2 -0.2
temperature shift Temperature shift 10_to_25; with MOPS +0.1 -0.3 +0.3 -3.9 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 -0.4 -0.4 -1.9 -0.4
phage P. put PUT 10 MOI 10 -1.6 +0.1 -0.3 -1.2 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 +0.5 -0.5 -1.9 -0.7
phage P. putida PUT 7 MOI 1 -0.3 -1.1 +0.4 -2.0 -0.1
temperature shift Temperature shift 10_to_25; with MOPS +0.3 -0.1 +0.1 -2.9 -0.3
pH Growth at pH6 and (C) Trisodium citrate -0.2 -0.6 -0.8 -1.3 -0.1
nitrogen source Propandiamine (N) -0.4 +0.0 -0.0 -2.4 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 -0.2 -0.0 -2.5 -0.1
carbon source Decanoic (C) -2.0 +0.0 +0.2 -0.5 -0.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.5 -0.2 +0.0 +0.6 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.4 +0.3 -0.2 -1.6 +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.3 +0.4 -0.1 +0.2 +0.1
phage P. putida South MOI 10 -1.6 -0.1 +0.4 -0.4 +0.3
phage P. putida PUT10 MOI 0.1 -1.8 -0.1 -0.1 +0.4 +0.3
phage PUT 8 MOI 1 -2.2 -0.1 +0.2 +0.2 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -1.6 -0.3 +0.0 +0.6 +0.5
phage P. putida PUT10 MOI 10 -1.7 +0.3 -0.1 +0.4 +0.3
phage P. putida PUT10 MOI 10 -1.6 +0.1 -0.1 +1.2 -0.2
phage PUT8 MOI 0.1 -2.4 +1.0 +0.1 +1.1 +0.1
phage PUT8 MOI 0.1 -2.6 +0.4 +0.3 +1.1 +0.8
dilutelb LB 0.1x with 55.5 mM D-Glucose +0.9 +0.4 -0.2 -1.2 +0.1
phage PUT8 MOI 10 -1.1 +0.7 +0.5 +0.6 +0.6
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.1 +0.2 +0.2 +1.7 -0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.1 -0.2 +2.2 +0.1
pH D-Glucose (C) pH 6 +0.3 +0.2 -0.2 +1.8 -0.1
nitrogen source 2,6-diaminopimelic (N) +0.4 +0.5 +0.7 -0.3 +0.9
nitrogen source D-2-Aminobutyric (N) -0.3 +0.6 -0.2 +1.6 +0.8
phage P. putida Sci small MOI 0.1 -0.4 +0.6 +1.0 +1.4 +0.1
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