Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1832 and PP_1833 are separated by 127 nucleotidesPP_1833 and PP_1834 are separated by 236 nucleotidesPP_1834 and PP_1835 are separated by 60 nucleotidesPP_1835 and PP_1836 are separated by 123 nucleotides PP_1832: PP_1832 - putative Oxidase, at 2,057,248 to 2,057,784 _1832 PP_1833: PP_1833 - conserved protein of unknown function, at 2,057,912 to 2,058,214 _1833 PP_1834: PP_1834 - conserved protein of unknown function, at 2,058,451 to 2,058,975 _1834 PP_1835: PP_1835 - conserved protein of unknown function, at 2,059,036 to 2,059,566 _1835 PP_1836: PP_1836 - putative metal transporter, ZIP family, at 2,059,690 to 2,060,628 _1836
Group Condition PP_1832 PP_1833 PP_1834 PP_1835 PP_1836
pH Growth at pH6 and (C) D-Glucose -0.8 -0.1 -0.9 +0.4 -3.8
nitrogen source NAG (N); with MOPS -0.1 +0.3 -0.9 +0.0 -2.9
nitrogen source 2,6-diaminopimelic (N) -1.1 -0.5 -0.9 -0.3 -0.5
pH Growth at pH9 and (C) Trisodium citrate -0.0 -1.7 -0.4 -0.4 -0.7
carbon source ferulate (C); with MOPS -0.8 -0.0 +0.4 -1.0 -1.4
pH Growth at pH6 and (C) Trisodium citrate -0.1 +0.0 -2.0 -0.5 -0.1
phage P. putida PUT 7 MOI 1 -1.1 -0.8 +0.5 -0.4 -0.6
nitrogen source nitrogen source nitrate 5mM -0.5 -0.2 -0.3 -0.1 -1.4
pH Growth at pH6 and (C) Trisodium citrate -0.0 -0.8 -0.4 +0.2 -1.1
phage P. putida Sci Big MOI 0.1 -0.7 -0.7 +0.5 -0.1 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 -1.2 -0.5 -0.4 -0.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.4 +0.3 -0.8 +0.3 -0.9
phage P. putida Sci small MOI 0.1 -0.0 +0.7 -0.6 -0.1 -1.4
phage P. put South MOI 10 +0.3 +0.0 -0.8 -1.1 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 -0.0 +0.5 -0.3 -1.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.9 -1.0 -0.7 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.6 +0.1 +0.6 +0.0 -1.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.1 -0.1 -0.7 +0.4 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.2 +0.3 +0.2 +0.1 -1.7
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -0.2 +0.4 +0.5 +0.0 -1.2
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.3 +0.8 -1.5 -0.4 +1.1
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay +0.3 +0.4 -0.6 +0.4 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 +0.2 +1.2 +0.1 -0.7
nitrogen source NAG (N); with MOPS +0.0 +0.4 +1.1 -0.1 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.4 +0.5 -0.0 +1.7
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.1 +0.8 +1.0 +0.2 -0.7
pH pH 5 -0.3 +0.7 +0.5 -0.0 +0.8
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.1 +0.6 +0.8 -0.2 +0.7
carbon source Nonanoic (C) +0.2 -0.3 +1.1 +0.1 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.5 +1.3 -0.4 +1.2
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