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Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1832 and PP_1833 are separated by 127 nucleotides
PP_1833 and PP_1834 are separated by 236 nucleotides
PP_1834 and PP_1835 are separated by 60 nucleotides
PP_1835 and PP_1836 are separated by 123 nucleotides
PP_1832: PP_1832 - putative Oxidase, at 2,057,248 to 2,057,784
_1832
PP_1833: PP_1833 - conserved protein of unknown function, at 2,057,912 to 2,058,214
_1833
PP_1834: PP_1834 - conserved protein of unknown function, at 2,058,451 to 2,058,975
_1834
PP_1835: PP_1835 - conserved protein of unknown function, at 2,059,036 to 2,059,566
_1835
PP_1836: PP_1836 - putative metal transporter, ZIP family, at 2,059,690 to 2,060,628
_1836
Group
Condition
PP
_1832
PP
_1833
PP
_1834
PP
_1835
PP
_1836
pH
Growth at pH6 and (C) D-Glucose
-0.8
-0.1
-0.9
+0.4
-3.8
nitrogen source
NAG (N); with MOPS
-0.1
+0.3
-0.9
+0.0
-2.9
nitrogen source
2,6-diaminopimelic (N)
-1.1
-0.5
-0.9
-0.3
-0.5
pH
Growth at pH9 and (C) Trisodium citrate
-0.0
-1.7
-0.4
-0.4
-0.7
carbon source
ferulate (C); with MOPS
-0.8
-0.0
+0.4
-1.0
-1.4
pH
Growth at pH6 and (C) Trisodium citrate
-0.1
+0.0
-2.0
-0.5
-0.1
phage
P. putida PUT 7 MOI 1
-1.1
-0.8
+0.5
-0.4
-0.6
nitrogen source
nitrogen source nitrate 5mM
-0.5
-0.2
-0.3
-0.1
-1.4
pH
Growth at pH6 and (C) Trisodium citrate
-0.0
-0.8
-0.4
+0.2
-1.1
phage
P. putida Sci Big MOI 0.1
-0.7
-0.7
+0.5
-0.1
-1.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.3
-1.2
-0.5
-0.4
-0.2
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.4
+0.3
-0.8
+0.3
-0.9
phage
P. putida Sci small MOI 0.1
-0.0
+0.7
-0.6
-0.1
-1.4
phage
P. put South MOI 10
+0.3
+0.0
-0.8
-1.1
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.3
-0.0
+0.5
-0.3
-1.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
+0.9
-1.0
-0.7
-0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
-0.6
+0.1
+0.6
+0.0
-1.4
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-1.1
-0.1
-0.7
+0.4
+0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
+0.2
+0.3
+0.2
+0.1
-1.7
stress
MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL
-0.2
+0.4
+0.5
+0.0
-1.2
carbon source
3-methyl-1-butanol 0.1 vol% (C)
-0.3
+0.8
-1.5
-0.4
+1.1
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
+0.3
+0.4
-0.6
+0.4
-0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
+0.2
+1.2
+0.1
-0.7
nitrogen source
NAG (N); with MOPS
+0.0
+0.4
+1.1
-0.1
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.4
-0.4
+0.5
-0.0
+1.7
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
+0.1
+0.8
+1.0
+0.2
-0.7
pH
pH 5
-0.3
+0.7
+0.5
-0.0
+0.8
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
-0.1
+0.6
+0.8
-0.2
+0.7
carbon source
Nonanoic (C)
+0.2
-0.3
+1.1
+0.1
+0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+0.5
+1.3
-0.4
+1.2
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