Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1682 and PP_1683 are separated by 62 nucleotidesPP_1683 and PP_1684 are separated by 44 nucleotidesPP_1684 and PP_1685 overlap by 4 nucleotidesPP_1685 and PP_1686 overlap by 11 nucleotides PP_1682: PP_1682 - putative glycoside hydrolase family protein, at 1,876,186 to 1,877,367 _1682 PP_1683: PP_1683 - Transcriptional regulator, MarR family, at 1,877,430 to 1,877,834 _1683 PP_1684: PP_1684 - putative Transporter, at 1,877,879 to 1,879,099 _1684 PP_1685: PP_1685 - conserved exported protein of unknown function, at 1,879,096 to 1,879,218 _1685 PP_1686: PP_1686 - Glutathione peroxidase, at 1,879,208 to 1,879,816 _1686
Group Condition PP_1682 PP_1683 PP_1684 PP_1685 PP_1686
nitrogen source NAG (N); with MOPS -0.1 -1.9 -0.9 N.D. -0.8
pH Valerolactone (C) pH 6.5 -0.1 -2.8 -0.3 N.D. +0.0
pH D-Glucose (C) pH 7.5 -0.0 -2.6 +0.1 N.D. -0.1
pH D-Glucose (C) pH 6.5 -0.1 -2.4 -0.2 N.D. -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.0 -2.9 +0.2 N.D. +0.1
carbon source Valerolactone (C) -0.2 -1.5 -0.6 N.D. -0.0
carbon source DL-3-Hydroxyisobutyric (C) +0.1 -2.8 +0.2 N.D. +0.2
nitrogen source NAG (N); with MOPS -0.2 -1.4 -0.4 N.D. -0.1
nitrogen source NAG (N) -0.2 -1.7 -0.1 N.D. -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -1.6 -0.1 N.D. -0.2
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL +0.1 -1.9 +0.0 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -1.2 -0.5 N.D. -0.4
temperature Growth at 25C -0.2 -1.4 -0.0 N.D. -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. +0.0 -1.7 -0.4 N.D. +0.2
phage P. putida PUT 7 MOI 1 -0.1 -1.8 +0.0 N.D. +0.1
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL +0.1 -1.5 -0.2 N.D. -0.1
nitrogen source 2,6-diaminopimelic (N) -0.2 -0.8 -0.6 N.D. +0.1
carbon source 2-Methylbutanoic (C) +0.1 -1.4 +0.1 N.D. -0.3
temperature shift Temperature shift 10_to_30; with MOPS +0.0 -1.6 +0.2 N.D. -0.1
carbon source DL-3-Hydroxybutyric (C) +0.1 -1.3 +0.2 N.D. -0.1
phage P. put South MOI 10 +0.4 -1.6 +0.0 N.D. +0.1
nitrogen source Urea (N); with MOPS -0.0 -1.3 +0.3 N.D. -0.0
stress Growth with Chloride 500 mM; with MOPS -0.0 -1.2 +0.4 N.D. +0.2
carbon source carbon source 4-vinylphenol 0.1 mM -0.1 -1.2 +0.3 N.D. +0.3
phage PUT 8 MOI 1 +0.1 -1.5 +0.4 N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 +0.6 -0.6 N.D. -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.0 +1.2 -0.5 N.D. +0.1
carbon source ferulate (C); with MOPS -0.3 +1.4 +0.3 N.D. -0.3
temperature shift Temperature shift 10_to_34; with MOPS +0.2 +1.1 -0.3 N.D. +0.1
nitrogen source nitrite (N) +0.0 +1.6 +0.1 N.D. +0.2
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