Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1556 and PP_1557 overlap by 28 nucleotidesPP_1557 and PP_1558 are separated by 10 nucleotidesPP_1558 and PP_1559 are separated by 199 nucleotidesPP_1559 and PP_1560 are separated by 60 nucleotides PP_1556: PP_1556 - conserved protein of unknown function, at 1,754,299 to 1,754,619 _1556 PP_1557: PP_1557 - conserved protein of unknown function, at 1,754,592 to 1,754,933 _1557 PP_1558: PP_1558 - conserved protein of unknown function, at 1,754,944 to 1,755,285 _1558 PP_1559: PP_1559 - Holin, at 1,755,485 to 1,755,808 _1559 PP_1560: PP_1560 - conserved protein of unknown function, at 1,755,869 to 1,756,051 _1560
Group Condition PP_1556 PP_1557 PP_1558 PP_1559 PP_1560
pH Growth at pH6 and (C) D-Glucose -0.1 +0.1 +0.1 -3.1 +0.7
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.3 -0.5 -0.1 -1.0 -0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.7 -0.4 -0.4 -1.0 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.1 -0.6 +0.5 -1.3 +0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 +0.4 -0.6 -0.7 +0.4
pH Growth at pH7 and (C) D-Glucose -0.5 +0.0 -0.5 +0.7 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 +0.6 -0.1 -0.8 -1.2
no stress control Growth with no stress +0.8 +0.2 -0.4 -1.1 -0.5
nitrogen source D-2-Aminobutyric (N) +0.6 -0.3 -1.0 -0.2 +0.4
nitrogen source Urea (N); with MOPS -0.4 -0.1 +0.3 +0.8 -1.1
phage P. putida Sci Big MOI 0.1 -0.5 +0.4 -0.2 -0.6 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -0.5 +0.6 +0.7 +0.1
nitrogen source Propiolactam (N) +1.2 +0.3 +0.5 -0.8 -0.6
pH Growth at pH6 and (C) Trisodium citrate +1.0 +0.3 +0.3 -0.8 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.5 -0.3 +0.5 +0.9 +0.3
nitrogen source D-2-Aminobutyric (N) +0.6 -0.3 -0.4 +1.3 -0.0
nitrogen source 2,6-diaminopimelic (N) -0.6 +0.9 +0.4 +0.9 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 +0.5 +0.2 +0.5 -0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 -0.4 -0.1 +1.6 -0.3
carbon source Valeric (C) +0.8 -0.1 -0.1 -0.5 +1.6
carbon source Lauric (C) +0.3 -0.2 -0.0 +0.6 +1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 -0.4 +0.4 +1.3 -0.3
nitrogen source no extra (N); with MOPS +0.8 +0.2 -0.1 +0.7 +0.7
carbon source ferulate (C); with MOPS +0.6 -0.1 +0.4 +1.3 +0.3
temperature shift Temperature shift 30_to_21; with MOPS +0.5 +0.4 +0.5 +0.7 +0.5
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.7 +0.6 +0.6 +0.7 +0.2
carbon source 1,3-Butandiol (C) +0.9 +0.2 -0.1 +0.4 +1.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.9 +0.0 +0.3 +0.7 +0.0
carbon source L-Leucine (C) +0.1 +0.7 +0.6 +0.7 +1.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +1.5 +0.8 +0.6 +0.5 +0.5
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