Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1517 and PP_1518 are separated by 48 nucleotidesPP_1518 and PP_1519 are separated by 33 nucleotidesPP_1519 and PP_1520 are separated by 76 nucleotidesPP_1520 and PP_1521 are separated by 69 nucleotides PP_1517: PP_1517 - RND efflux transporter, at 1,721,368 to 1,724,433 _1517 PP_1518: PP_1518 - conserved exported protein of unknown function, at 1,724,482 to 1,725,168 _1518 PP_1519: PP_1519 - putative Lipoprotein, at 1,725,202 to 1,725,600 _1519 PP_1520: PP_1520 - glycerol-3-phosphate acyltransferase, at 1,725,677 to 1,728,163 _1520 PP_1521: PP_1521 - conserved protein of unknown function, at 1,728,233 to 1,728,373 _1521
Group Condition PP_1517 PP_1518 PP_1519 PP_1520 PP_1521
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs +0.1 -0.6 -0.7 -0.2 -1.8
phage P. putida Sci small MOI 0.1 -0.1 -1.8 +0.3 -0.3 -1.0
phage P. putida Missley MOI 0.1 -0.0 -0.4 -0.3 +0.2 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 -0.7 +0.2 -0.3 -1.2
carbon source DL-2-Aminoadipic 10 mM (C) -0.1 -0.4 -0.1 -0.2 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.4 +0.1 -0.8 +0.1 -0.5
pH pH 7 +0.0 -0.5 -0.9 +0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.3 -0.2 -0.0 -1.0
phage P. putida Sci Big MOI 0.1 -0.2 +0.5 -1.5 -0.0 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.3 -0.6 -0.3 -0.1 -0.5
pH Growth at pH7 and (C) D-Glucose -0.6 -0.2 -0.4 +0.2 -0.3
phage P. putida South MOI 10 +0.2 -0.0 +0.0 +0.1 -1.3
temperature Growth at 30C; with MOPS -0.1 +0.3 -0.0 -0.2 -1.0
carbon source Valeric (C) -0.2 -0.0 -0.7 -0.4 +0.3
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.1 -0.2 +0.4 -0.1 -0.8
carbon source 5-Aminovaleric 10 mM (C) -0.2 -0.7 -0.4 +0.2 +0.3
nitrogen source D-2-Aminobutyric (N) +0.4 -0.2 -0.1 +0.1 -1.0
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.4 -0.6 +0.2 +0.1 -0.8
temperature shift Temperature shift 30_to_25 +0.3 -0.2 -0.5 +0.3 -0.5
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM +0.2 +0.2 -0.3 +0.3 -0.8
temperature shift Temperature shift 10_to_25; with MOPS +0.2 -0.4 -0.5 -0.1 +0.5
nitrogen source 2,6-diaminopimelic (N) -0.2 -0.8 +0.2 -0.3 +0.8
carbon source ferulate (C); with MOPS -0.1 +0.4 -0.7 +0.4 +0.0
carbon source ferulate (C); with MOPS -0.1 +0.5 +0.2 +0.3 -0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.3 -0.4 +0.3 -0.0 +0.7
pH Growth at pH6 and (C) D-Glucose -0.1 -0.2 -0.2 -0.2 +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.4 +0.7 +0.2 +0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.2 -0.0 +0.6 -0.2 +0.7
stress Formaldehyde 0.03125 vol% -0.1 +0.5 +0.4 +0.0 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 +0.2 +0.1 +0.2 +1.2
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