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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1517 and PP_1518 are separated by 48 nucleotides
PP_1518 and PP_1519 are separated by 33 nucleotides
PP_1519 and PP_1520 are separated by 76 nucleotides
PP_1520 and PP_1521 are separated by 69 nucleotides
PP_1517: PP_1517 - RND efflux transporter, at 1,721,368 to 1,724,433
_1517
PP_1518: PP_1518 - conserved exported protein of unknown function, at 1,724,482 to 1,725,168
_1518
PP_1519: PP_1519 - putative Lipoprotein, at 1,725,202 to 1,725,600
_1519
PP_1520: PP_1520 - glycerol-3-phosphate acyltransferase, at 1,725,677 to 1,728,163
_1520
PP_1521: PP_1521 - conserved protein of unknown function, at 1,728,233 to 1,728,373
_1521
Group
Condition
PP
_1517
PP
_1518
PP
_1519
PP
_1520
PP
_1521
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
+0.1
-0.6
-0.7
-0.2
-1.8
phage
P. putida Sci small MOI 0.1
-0.1
-1.8
+0.3
-0.3
-1.0
phage
P. putida Missley MOI 0.1
-0.0
-0.4
-0.3
+0.2
-1.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.1
-0.7
+0.2
-0.3
-1.2
carbon source
DL-2-Aminoadipic 10 mM (C)
-0.1
-0.4
-0.1
-0.2
-0.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
-0.4
+0.1
-0.8
+0.1
-0.5
pH
pH 7
+0.0
-0.5
-0.9
+0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.3
-0.2
-0.0
-1.0
phage
P. putida Sci Big MOI 0.1
-0.2
+0.5
-1.5
-0.0
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.3
-0.6
-0.3
-0.1
-0.5
pH
Growth at pH7 and (C) D-Glucose
-0.6
-0.2
-0.4
+0.2
-0.3
phage
P. putida South MOI 10
+0.2
-0.0
+0.0
+0.1
-1.3
temperature
Growth at 30C; with MOPS
-0.1
+0.3
-0.0
-0.2
-1.0
carbon source
Valeric (C)
-0.2
-0.0
-0.7
-0.4
+0.3
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.1
-0.2
+0.4
-0.1
-0.8
carbon source
5-Aminovaleric 10 mM (C)
-0.2
-0.7
-0.4
+0.2
+0.3
nitrogen source
D-2-Aminobutyric (N)
+0.4
-0.2
-0.1
+0.1
-1.0
carbon source
3-methyl-1-butanol 0.1 vol% (C)
+0.4
-0.6
+0.2
+0.1
-0.8
temperature shift
Temperature shift 30_to_25
+0.3
-0.2
-0.5
+0.3
-0.5
mixed carbon source
mixed (C)s Trisodium citrate 10 mM and L-Meta-tyrosine 10 mM
+0.2
+0.2
-0.3
+0.3
-0.8
temperature shift
Temperature shift 10_to_25; with MOPS
+0.2
-0.4
-0.5
-0.1
+0.5
nitrogen source
2,6-diaminopimelic (N)
-0.2
-0.8
+0.2
-0.3
+0.8
carbon source
ferulate (C); with MOPS
-0.1
+0.4
-0.7
+0.4
+0.0
carbon source
ferulate (C); with MOPS
-0.1
+0.5
+0.2
+0.3
-0.7
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.3
-0.4
+0.3
-0.0
+0.7
pH
Growth at pH6 and (C) D-Glucose
-0.1
-0.2
-0.2
-0.2
+1.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
-0.4
+0.7
+0.2
+0.6
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.2
-0.0
+0.6
-0.2
+0.7
stress
Formaldehyde 0.03125 vol%
-0.1
+0.5
+0.4
+0.0
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.0
+0.2
+0.1
+0.2
+1.2
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