Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1500 and PP_1501 overlap by 4 nucleotidesPP_1501 and PP_1502 are separated by 141 nucleotidesPP_1502 and PP_1503 are separated by 50 nucleotidesPP_1503 and PP_1504 are separated by 219 nucleotides PP_1500: PP_1500 - putative Lipoprotein, at 1,705,936 to 1,706,859 _1500 PP_1501: PP_1501 - conserved protein of unknown function, at 1,706,856 to 1,707,182 _1501 PP_1502: PP_1502 - OmpA family protein, at 1,707,324 to 1,708,115 _1502 PP_1503: PP_1503 - conserved protein of unknown function, at 1,708,166 to 1,708,636 _1503 PP_1504: PP_1504 - conserved protein of unknown function, at 1,708,856 to 1,709,233 _1504
Group Condition PP_1500 PP_1501 PP_1502 PP_1503 PP_1504
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.2 -1.1 -1.8 -0.2
carbon source ferulate (C); with MOPS +0.1 +0.3 -1.3 -1.3 -0.4
pH Growth at pH7 and (C) Trisodium citrate -0.1 -0.1 -0.6 -1.3 -0.1
carbon source carbon source 4-vinylphenol 0.01 mM -0.2 -0.7 -0.7 -0.9 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.3 -0.9 -0.1 -0.9 +0.2
pH Growth at pH8 and (C) D-Glucose +0.1 +0.1 +0.0 -1.9 -0.2
pH pH 7 -0.1 -0.5 -0.5 -1.1 +0.7
pH Growth at pH8 and (C) Trisodium citrate +0.3 +0.0 -0.6 -1.3 +0.1
phage P. putida Sci Big MOI 0.1 -0.8 +0.4 +0.2 -0.3 -0.8
phage P. putida Missley MOI 0.1 -0.1 +0.2 -0.9 -0.6 +0.2
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.0 -0.7 -0.2 -0.9 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.3 +0.4 -0.8 -0.2
carbon source carbon source 4-vinylphenol 0.1 mM -0.2 -0.9 -0.3 -0.2 +0.5
pH Growth at pH6 and (C) D-Glucose +0.5 -1.4 -0.4 +1.1 -0.7
phage P. putida Sci small MOI 0.1 +0.0 -0.8 +0.3 +0.4 -0.9
carbon source carbon source 3-O-methyl-glucose 10 mM -0.2 -0.0 -0.4 -1.0 +0.6
carbon source Valeric (C) -0.5 -0.5 -0.7 +0.7 +0.2
phage P. putida Sci small MOI 0.1 -0.0 -0.5 +0.0 +0.8 -1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.9 -0.2 -0.6 +0.6
pH pH 6 -0.4 -0.5 -0.6 +0.5 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.4 -0.1 +0.5 -1.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.4 +0.4 -0.5 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.0 -1.0 +0.5 +0.6 -0.2
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.0 -1.0 -0.2 +0.6 +0.5
carbon source butyrate (C) +0.0 -0.7 -0.2 +1.3 +0.3
stress MOPS minimal media_Glucose20mM with Lithium chloride 200 mM +0.4 +0.1 +0.4 -0.7 +0.5
temperature shift Temperature shift 30_to_21; with MOPS +0.4 +0.4 -0.4 +0.7 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.0 +0.8 -0.0 +1.2 -0.2
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.2 +0.3 -0.1 +1.0 +1.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.2 +0.2 +1.8 +0.5
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