Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1471 and PP_5471 are separated by 130 nucleotidesPP_5471 and PP_1473 are separated by 79 nucleotidesPP_1473 and PP_1474 are separated by 186 nucleotidesPP_1474 and PP_1475 are separated by 20 nucleotides PP_1471: PP_1471 - Threonine synthase, at 1,676,198 to 1,677,607 _1471 PP_5471: PP_5471 - putative response regulator receiver protein, at 1,677,738 to 1,678,157 _5471 PP_1473: PP_1473 - conserved protein of unknown function, at 1,678,237 to 1,678,518 _1473 PP_1474: PP_1474 - conserved exported protein of unknown function, at 1,678,705 to 1,679,580 _1474 PP_1475: PP_1475 - CAIB/BAIF family protein, at 1,679,601 to 1,680,800 _1475
Group Condition PP_1471 PP_5471 PP_1473 PP_1474 PP_1475
nitrogen source NAG (N); with MOPS N.D. -4.4 +0.1 -0.2 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -3.9 -0.5 -0.1 +0.1
phage P. putida PUT 7 MOI 1 N.D. -2.7 -1.2 +0.2 -0.5
phage P. putida Missley MOI 0.1 N.D. -3.9 -0.4 +0.0 +0.3
nitrogen source Ammonium chloride (N) N.D. -2.9 -0.5 -0.5 +0.5
stress R2A with Fusaric 240 ug/mL N.D. -2.3 -0.5 -0.1 -0.4
pH Growth at pH6 and (C) Trisodium citrate N.D. -1.5 -1.5 -0.2 -0.1
temperature shift Temperature shift 30_to_25; with MOPS N.D. -2.4 -0.7 -0.2 +0.3
temperature Growth at 25C N.D. -1.8 +0.1 -0.6 -0.7
pH Growth at pH7 and (C) Trisodium citrate N.D. -2.3 -0.4 +0.1 -0.3
nitrogen source nitrogen source Ammonium chloride 20mM N.D. -1.2 -0.9 -0.1 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs N.D. -1.9 -0.2 -0.4 +0.1
nitrogen source nitrogen source nitrite 1mM N.D. -1.2 -1.2 -0.3 +0.2
temperature shift Temperature shift 10_to_25; with MOPS N.D. -2.9 +0.5 -0.0 +0.5
r2a control with 0.2x vogels R2A control with 0.2X Vogels N.D. -1.7 +0.3 +0.2 -0.7
micoeukaryotes C.elegans, mixed culture-2 N.D. -2.5 -0.3 +0.5 +0.5
nitrogen source 2,6-diaminopimelic (N) N.D. -1.8 -0.1 +0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -1.7 -0.3 +0.2 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. +0.7 -0.7 -0.4 -0.8
pH Growth at pH6 and (C) D-Glucose N.D. -2.4 +0.5 +0.4 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. +1.3 -0.9 -0.3 -0.4
carbon source 4-Aminobutyric 10 mM (C) N.D. +1.5 +0.2 -0.4 -1.1
pH pH 9 N.D. +1.6 -0.3 -0.4 -0.3
carbon source carbon source D-Glucose 55.5 mM N.D. +1.8 +0.1 -0.1 -1.2
carbon source carbon source 2-Deoxy-D-glucose 10 mM N.D. +1.6 +0.0 -0.4 -0.4
carbon source carbon source 3-O-methyl-glucose 10 mM N.D. +1.6 +0.3 -0.4 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. +0.9 -0.4 +0.7 +0.4
phage P. putida Sci small MOI 0.1 N.D. +1.3 +0.8 -0.4 -0.1
pH pH 7 N.D. +2.1 +0.1 +0.1 -0.4
carbon source acetate (C) N.D. +0.8 +0.9 -0.1 +0.7
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