Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1462 and PP_1463 are separated by 5 nucleotidesPP_1463 and PP_1464 are separated by 3 nucleotidesPP_1464 and PP_1465 are separated by 42 nucleotidesPP_1465 and PP_1466 are separated by 94 nucleotides PP_1462: PP_1462 - 30S ribosomal protein S16, at 1,668,557 to 1,668,886 _1462 PP_1463: PP_1463 - Ribosome maturation factor RimM, at 1,668,892 to 1,669,428 _1463 PP_1464: PP_1464 - tRNA (guanine-N(1)-)-methyltransferase, at 1,669,432 to 1,670,184 _1464 PP_1465: PP_1465 - 50S ribosomal protein L19, at 1,670,227 to 1,670,577 _1465 PP_1466: PP_1466 - Long-chain acyl-CoA thioester hydrolase family protein, at 1,670,672 to 1,671,079 _1466
Group Condition PP_1462 PP_1463 PP_1464 PP_1465 PP_1466
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. N.D. -2.3
phage P. putida PUT 7 MOI 1 N.D. N.D. N.D. N.D. -2.0
carbon source 3-methyl-1-butanol 0.25 vol% (C) N.D. N.D. N.D. N.D. -1.8
temperature shift Temperature shift 30_to_21; with MOPS N.D. N.D. N.D. N.D. -1.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. N.D. N.D. N.D. -1.3
carbon source DL-3-Hydroxybutyric (C) N.D. N.D. N.D. N.D. -1.3
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. N.D. N.D. N.D. -1.3
pH D-Glucose (C) pH 7 N.D. N.D. N.D. N.D. -1.2
no stress control Growth with no stress N.D. N.D. N.D. N.D. -1.2
pH Growth at pH6 and (C) D-Glucose N.D. N.D. N.D. N.D. -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. N.D. -1.2
temperature shift Temperature shift 10_to_25; with MOPS N.D. N.D. N.D. N.D. -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. N.D. -1.1
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL N.D. N.D. N.D. N.D. -1.0
carbon source 2-Methylbutanoic (C) N.D. N.D. N.D. N.D. -0.9
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM N.D. N.D. N.D. N.D. -0.9
nitrogen source Propiolactam (N) N.D. N.D. N.D. N.D. +0.9
carbon source Butanol (C) N.D. N.D. N.D. N.D. +0.9
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. N.D. N.D. N.D. N.D. +0.9
stress Growth with Chloride 200 mM; with MOPS N.D. N.D. N.D. N.D. +0.9
pH Growth at pH9 and (C) Trisodium citrate N.D. N.D. N.D. N.D. +0.9
nitrogen source NAG (N); with MOPS N.D. N.D. N.D. N.D. +0.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. N.D. N.D. N.D. +0.9
carbon source ferulate (C); with MOPS N.D. N.D. N.D. N.D. +1.0
nitrogen source 2,6-diaminopimelic (N) N.D. N.D. N.D. N.D. +1.0
pH Growth at pH6 and (C) D-Glucose N.D. N.D. N.D. N.D. +1.1
phage PUT8 MOI 10 N.D. N.D. N.D. N.D. +1.1
pH Valerolactone (C) pH 6.5 N.D. N.D. N.D. N.D. +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. N.D. +1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. N.D. +1.9
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