Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1446 and PP_1447 are separated by 136 nucleotidesPP_1447 and PP_1448 are separated by 1 nucleotidesPP_1448 and PP_1449 are separated by 160 nucleotidesPP_1449 and PP_1450 are separated by 27 nucleotides PP_1446: PP_1446 - TonB-dependent receptor, at 1,649,017 to 1,651,059 _1446 PP_1447: PP_1447 - conserved protein of unknown function, at 1,651,196 to 1,651,444 _1447 PP_1448: PP_1448 - conserved membrane protein of unknown function, at 1,651,446 to 1,652,081 _1448 PP_1449: PP_1449 - conserved exported protein of unknown function, at 1,652,242 to 1,656,768 _1449 PP_1450: PP_1450 - putative Activation/secretion protein, TPS family, at 1,656,796 to 1,658,493 _1450
Group Condition PP_1446 PP_1447 PP_1448 PP_1449 PP_1450
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -2.0 -0.2 -0.1 +0.1
carbon source L-Leucine (C) -0.3 -1.0 -0.2 -0.3 +0.1
nitrogen source NAG (N); with MOPS -0.2 -0.8 +0.1 -0.0 -0.5
pH Growth at pH7 and (C) D-Glucose -0.3 -0.8 -0.1 +0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.1 -1.1 -0.1 -0.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -1.1 -0.0 -0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 -1.8 -0.1 +0.2 +0.1
r2a control with 0.2x vogels R2A control with 0.2X Vogels +0.1 -0.7 -0.5 -0.2 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 -0.5 -0.5 -0.2 -0.1
carbon source L-Leucine (C) -0.2 -0.6 -0.3 +0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 -0.3 -0.5 -0.1 +0.1
pH Growth at pH6 and (C) D-Glucose -0.0 -1.0 -0.3 +0.3 +0.0
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.1 -0.3 +0.2 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs -0.3 -0.8 -0.2 +0.1 +0.3
stress Growth with Chloride 500 mM; with MOPS -0.2 -0.8 +0.3 +0.1 -0.2
stress Growth with Chloride 200 mM -0.2 -0.6 +0.3 +0.0 -0.3
phage P. putida PUT 7 MOI 1 +0.3 -0.4 -0.8 +0.2 -0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 -0.5 -0.3 +0.1 -0.3
nitrogen source Urea (N) -0.1 +0.4 -0.3 -0.3 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.2 -0.8 -0.1 -0.0 +0.2
pH Growth at pH7 and (C) Trisodium citrate -0.7 +0.3 -0.2 +0.1 +0.1
carbon source L-Lysine (C) -0.5 +0.7 -0.4 -0.1 -0.1
nitrogen source nitrite (N) -0.2 -0.5 -0.2 +0.3 +0.2
carbon source Trisodium citrate (C) -0.3 +0.2 +0.4 -0.3 -0.3
nitrogen source D-2-Aminobutyric (N) +0.2 -0.8 +0.5 -0.1 +0.2
temperature shift Temperature shift 30_to_4; with MOPS -0.3 +0.6 +0.2 -0.0 -0.1
pH Growth at pH7 and (C) Trisodium citrate -0.3 +0.3 +0.5 +0.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.7 -0.2 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +0.5 +0.4 -0.2 +0.3
micoeukaryotes Tetrahymena sp., Mixed culture-2 +0.1 +0.9 +0.5 +0.3 +0.0
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