Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1411 and PP_5470 are separated by 212 nucleotidesPP_5470 and PP_1412 are separated by 44 nucleotidesPP_1412 and PP_1413 are separated by 126 nucleotidesPP_1413 and PP_1414 are separated by 26 nucleotides PP_1411: PP_1411 - GGDEF domain protein, at 1,608,783 to 1,610,264 _1411 PP_5470: PP_5470 - conserved protein of unknown function, at 1,610,477 to 1,610,926 _5470 PP_1412: PP_1412 - 3-hydroxyisobutyryl-CoA hydrolase, at 1,610,971 to 1,612,077 _1412 PP_1413: PP_1413 - Uracil-DNA glycosylase, at 1,612,204 to 1,612,896 _1413 PP_1414: PP_1414 - 5'-nucleotidase - 2',3'-cyclic phosphodiesterase, at 1,612,923 to 1,614,605 _1414
Group Condition PP_1411 PP_5470 PP_1412 PP_1413 PP_1414
nitrogen source NAG (N); with MOPS -0.1 +0.3 -0.9 +0.1 -0.1
temperature shift Temperature shift 30_to_21; with MOPS -0.1 +0.1 -0.1 -0.8 +0.2
stress R2A with Fusaric 240 ug/mL -0.2 -0.1 -0.5 +0.3 -0.2
carbon source Valeric (C) -0.1 +0.2 -0.8 +0.1 -0.1
stress Geraniol 1 vol% -0.5 +0.3 -0.3 -0.1 +0.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.0 -0.1 -0.7 +0.3 +0.0
carbon source carbon source 4-vinylphenol 0.01 mM -0.1 -0.0 -0.4 +0.5 -0.2
phage P. putida Sci small MOI 0.1 +0.2 +0.1 +0.2 -0.7 +0.1
micoeukaryotes Acanthamoeba sp., muxed culture-1 -0.4 +0.3 +0.3 -0.2 -0.0
mixed carbon source mixed (C)s Trisodium citrate 10 mM and 3-Chloro-L-tyrosine 10 mM -0.0 +0.2 +0.2 -0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.0 -0.3 +0.6 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.1 +0.1 -0.6 +0.9 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.2 -0.2 +0.6 -0.0
carbon source carbon source 3-O-methyl-glucose 10 mM -0.0 -0.0 +0.5 +0.3 -0.4
carbon source Delta-Nonalactone (C) -0.1 -0.0 -0.3 +0.7 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 +0.1 -0.3 +0.5 -0.1
carbon source Nonanoic (C) +0.0 +0.1 -0.3 +0.7 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 +0.2 -0.4 +0.8 -0.0
stress Isobutyl alcohol 0.6 vol% -0.1 -0.0 -0.2 +0.8 +0.1
carbon source Myristic (C) +0.0 +0.5 -0.3 +0.3 +0.2
carbon source L-Leucine (C) +0.2 -0.2 +0.4 +0.5 -0.2
carbon source ferulate (C); with MOPS +0.2 +0.0 -0.2 +0.4 +0.3
pH pH 8 +0.2 +0.2 +0.4 +0.3 -0.2
pH pH 6 +0.1 +0.4 +0.4 +0.6 -0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.3 +0.2 +0.0 +0.6 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.0 +0.9 +0.2 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.1 +1.2 +0.0 -0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.1 +0.7 +1.0 -0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +1.3 +0.4 -0.3 -0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 +1.2 +0.6 +0.6 -0.1
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