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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1402 and PP_1403 are separated by 130 nucleotides
PP_1403 and PP_1404 are separated by 186 nucleotides
PP_1404 and PP_5469 are separated by 166 nucleotides
PP_5469 and PP_1406 are separated by 32 nucleotides
PP_1402: PP_1402 - C4-dicarboxylate transport sensor protein, at 1,597,004 to 1,598,761
_1402
PP_1403: PP_1403 - periplasmic beta-D-glucoside glucohydrolase, at 1,598,892 to 1,601,183
_1403
PP_1404: PP_1404 - conserved protein of unknown function, at 1,601,370 to 1,601,987
_1404
PP_5469: PP_5469 - putative SAM-dependent methyltransferase, at 1,602,154 to 1,603,368
_5469
PP_1406: PP_1406 - conserved protein of unknown function, at 1,603,401 to 1,604,411
_1406
Group
Condition
PP
_1402
PP
_1403
PP
_1404
PP
_5469
PP
_1406
carbon source
Glutaric (C)
-4.6
-1.5
-0.3
+0.2
-0.0
carbon source
Glutaric (C)
-4.4
-1.3
-0.2
+0.1
+0.2
carbon source
a-Ketoglutaric (C)
-2.6
-1.0
-0.1
-0.1
-0.2
pH
Growth at pH7 and (C) Trisodium citrate
+0.1
-0.1
-3.5
-0.3
-0.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs
-0.2
+0.1
-0.7
-1.7
-0.2
carbon source
1,5-Pentanediol (C)
-2.0
-0.6
-0.1
+0.2
-0.1
carbon source
Trisodium citrate (C)
-0.3
-0.0
-1.9
-0.5
+0.1
phage
P. putida Sci small MOI 0.1
+0.0
-0.1
-1.9
-0.1
-0.3
carbon source
1,5-Pentanediol (C)
-2.1
-0.4
-0.2
+0.3
-0.0
carbon source
Valerolactone (C)
-1.2
-0.5
-0.3
+0.0
-0.2
pH
Valerolactone (C) pH 6.5
-1.5
+0.1
-1.7
+0.9
+0.1
carbon source
D-Glucose (C)
-0.1
-0.2
-2.1
+0.4
-0.0
nitrogen source
NAG (N); with MOPS
+0.3
-0.2
-1.7
-0.1
-0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.2
-1.4
-0.6
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.0
-0.2
-1.6
-0.3
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.6
+0.1
+0.1
-1.5
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
-0.2
-0.9
-0.8
+0.3
carbon source
Valerolactone (C)
-1.7
-0.3
+0.1
+0.6
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.2
-0.1
-1.4
-0.4
+0.3
pH
Growth at pH9 and (C) Trisodium citrate
-0.3
-0.2
-0.4
+0.7
-0.7
temperature
Growth at 30C; with MOPS
-0.2
-0.2
+0.7
-0.7
-0.3
nitrogen source
nitrogen source nitrite 1mM
-0.2
-0.0
-1.0
+0.9
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.3
-0.7
+0.7
-0.3
phage
P. putida PUT10 MOI 10
+0.0
+0.4
+0.6
-0.7
-0.5
nitrogen source
D-2-Aminobutyric (N)
-0.5
+0.5
+0.9
-0.3
-0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
+1.0
-0.3
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
-0.2
+1.2
-0.4
+0.3
pH
pH 7
-0.3
-0.2
+1.1
+0.6
+0.1
pH
Growth at pH6 and (C) D-Glucose
+0.3
+0.1
+0.9
+0.6
-0.2
stress
Geraniol 1 vol%
+0.9
+0.9
+0.4
+0.2
+0.2
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