Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1344 and PP_1345 are separated by 114 nucleotidesPP_1345 and PP_1346 are separated by 182 nucleotidesPP_1346 and PP_1347 are separated by 155 nucleotidesPP_1347 and PP_1348 overlap by 4 nucleotides PP_1344: PP_1344 - conserved protein of unknown function, at 1,531,246 to 1,531,875 _1344 PP_1345: PP_1345 - protein translocation complex, ATPase subunit SecA, at 1,531,990 to 1,534,809 _1345 PP_1346: PP_1346 - Glutamate N-acetyltransferase/Amino-acid acetyltransferase, at 1,534,992 to 1,536,209 _1346 PP_1347: PP_1347 - Glutathione S-transferase family protein, at 1,536,365 to 1,536,997 _1347 PP_1348: PP_1348 - putative MutT/nudix family protein/thiamine-phosphate pyrophosphorylase, at 1,536,994 to 1,537,938 _1348
Group Condition PP_1344 PP_1345 PP_1346 PP_1347 PP_1348
nitrogen source D-2-Aminobutyric (N) -1.8 N.D. -1.0 -0.2 -4.9
nitrogen source Propandiamine (N) -0.4 N.D. -1.7 -0.4 -4.6
temperature Growth at 30C; with MOPS -4.3 N.D. -1.3 +0.2 -1.4
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -3.8 N.D. -0.1 -0.2 -2.3
carbon source L-Phenylalanine 5 mM (C) -2.1 N.D. -0.6 +0.1 -3.7
nitrogen source Propandiamine (N) -0.1 N.D. -1.2 -0.5 -4.4
pH Growth at pH6 and (C) D-Glucose -4.3 N.D. -0.3 -0.3 -0.9
nitrogen source D-2-Aminobutyric (N) -0.5 N.D. -0.4 -0.4 -4.4
carbon source 3-methyl-1-butanol 0.1 vol% (C) +0.3 N.D. -1.0 -1.2 -3.7
pH pH 9 -0.9 N.D. -0.4 -0.3 -3.8
pH pH 5 +0.1 N.D. -0.3 -0.1 -4.9
mixed carbon source mixed (C)s Trisodium citrate 10 mM and L-Phenylalanine 5 mM -1.4 N.D. -0.8 -0.5 -2.6
carbon source carbon source Bisabolene 48.9 mM -0.5 N.D. +0.4 -0.2 -4.8
phage PUT 8 MOI 1 -4.7 N.D. +0.0 -0.4 +0.1
carbon source ferulate (C); with MOPS +0.2 N.D. -1.0 -0.7 -3.4
pH pH 4 +0.2 N.D. +0.0 -0.6 -4.4
nitrogen source NAG (N); with MOPS -4.0 N.D. -0.1 +0.4 -1.0
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL -1.7 N.D. +0.2 -0.2 -3.0
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. +0.6 N.D. -1.4 -0.2 -3.6
carbon source carbon source Glycerol 108.6 mM -1.0 N.D. +0.3 +0.1 -4.0
carbon source carbon source 2-Deoxy-D-glucose 50 mM +0.4 N.D. -0.3 -0.5 -3.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -4.1 N.D. -0.6 +0.5 +0.0
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.5 N.D. +0.4 -0.1 -4.9
pH pH 7 +0.0 N.D. +0.6 -0.9 -3.8
carbon source 2,3-Butanediol (C) +0.6 N.D. -0.3 -0.4 -3.9
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.5 N.D. +0.3 -0.9 -3.7
carbon source carbon source 3-O-methyl-glucose 10 mM +1.0 N.D. +0.4 -0.2 -4.6
nitrogen source DL-2-Aminobutyric (N) +1.9 N.D. -0.8 -0.1 -3.0
pH Growth at pH9 and (C) Trisodium citrate +1.6 N.D. -0.6 -0.7 -2.1
carbon source D-Glucose (C) +2.0 N.D. -1.4 -0.6 -1.7
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