Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1271 and PP_1272 overlap by 14 nucleotidesPP_1272 and PP_1273 overlap by 4 nucleotidesPP_1273 and PP_1274 are separated by 248 nucleotidesPP_1274 and PP_1275 are separated by 31 nucleotides PP_1271: PP_1271 - putative Multidrug efflux MFS transporter, at 1,451,839 to 1,453,380 _1271 PP_1272: PP_1272 - putative Multidrug efflux MFS membrane fusion protein, at 1,453,367 to 1,454,470 _1272 PP_1273: PP_1273 - putative Multidrug efflux MFS outer membrane protein, at 1,454,467 to 1,455,921 _1273 PP_1274: PP_1274 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 1,456,170 to 1,456,982 _1274 PP_1275: PP_1275 - conserved protein of unknown function, at 1,457,014 to 1,457,478 _1275
Group Condition PP_1271 PP_1272 PP_1273 PP_1274 PP_1275
stress R2A with Fusaric 480 ug/mL -1.7 -0.7 -1.3 -0.1 -0.4
stress R2A with Fusaric 480 ug/mL -0.8 -0.9 -1.0 -0.2 -0.4
stress R2A with Fusaric 480 ug/mL -1.1 -1.0 -0.8 +0.1 -0.2
stress R2A with Fusaric 480 ug/mL -0.8 -0.8 -0.8 -0.1 -0.3
stress R2A with Fusaric 480 ug/mL -1.0 -0.9 -0.6 -0.1 -0.1
stress R2A with Fusaric 480 ug/mL -1.6 -0.4 -0.5 +0.1 -0.2
carbon source 4-Aminobutyric 10 mM (C) -1.7 -0.2 -0.3 -0.3 -0.1
carbon source carbon source 4-vinylphenol 0.01 mM -1.0 +0.0 +0.3 -0.4 -0.5
temperature shift Temperature shift 30_to_25; with MOPS -0.8 -0.3 -0.3 +0.2 -0.3
nitrogen source 2,6-diaminopimelic (N) +0.7 -0.0 -1.0 +0.1 -1.1
pH Growth at pH6 and (C) D-Glucose -1.0 +0.0 -0.2 -0.4 +0.4
phage P. putida Sci small MOI 0.1 -0.2 -0.7 -0.9 +0.5 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 -0.2 +0.4 -0.9 -0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.1 +0.2 -0.4 +0.4 -0.2
no stress control Growth with no stress +0.4 -0.3 -0.3 +0.1 -0.8
pH pH 7 -0.6 -0.0 -0.0 -0.8 +0.5
carbon source carbon source Glycerol 108.6 mM -1.0 +0.2 +0.1 -0.5 +0.4
temperature Growth at 25C -1.0 -0.3 +0.1 +0.2 +0.4
nitrogen source D-2-Aminobutyric (N) -1.4 -0.1 +0.0 +0.4 +0.6
pH Growth at pH6 and (C) D-Glucose +0.4 +0.1 -0.1 +0.4 -1.3
carbon source carbon source 2-Deoxy-D-glucose 50 mM -1.0 +0.0 +0.5 -0.2 +0.3
phage P. putida Missley MOI 0.1 +0.7 +0.2 -0.4 -0.2 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -1.0 -0.1 -0.2 +0.4 +0.7
carbon source carbon source 3-O-methyl-glucose 50 mM -0.0 +0.9 +0.1 -1.1 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs +0.6 -0.3 +0.1 -0.5 +0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.1 -0.5 -0.3 +1.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.0 +0.4 -0.6 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 -0.1 +0.2 +0.4 -0.7
pH Growth at pH8 and (C) D-Glucose +0.6 -0.4 +0.1 +0.4 +0.4
nitrogen source nitrogen source nitrite 1mM -0.3 -0.0 +0.2 +0.8 +0.6
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