Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1250 and PP_1251 are separated by 375 nucleotidesPP_1251 and PP_1252 are separated by 479 nucleotidesPP_1252 and PP_1253 are separated by 151 nucleotidesPP_1253 and PP_1254 are separated by 49 nucleotides PP_1250: PP_1250 - Group II intron-encoding maturase, at 1,426,103 to 1,427,524 _1250 PP_1251: PP_1251 - malate:quinone oxidoreductase, at 1,427,900 to 1,429,483 _1251 PP_1252: PP_1252 - Group II intron-encoding maturase, at 1,429,963 to 1,431,384 _1252 PP_1253: PP_1253 - Transcriptional regulator, ArsR family, at 1,431,536 to 1,431,871 _1253 PP_1254: PP_1254 - XenA, at 1,431,921 to 1,433,012 _1254
Group Condition PP_1250 PP_1251 PP_1252 PP_1253 PP_1254
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs N.D. -0.1 N.D. -1.4 -0.2
stress R2A with Fusaric 240 ug/mL N.D. -0.0 N.D. -0.6 -1.0
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. N.D. -0.3 N.D. -0.2 -1.0
carbon source ferulate (C); with MOPS N.D. -0.0 N.D. -0.6 -0.8
nitrogen source Urea (N); with MOPS N.D. -0.2 N.D. -0.8 -0.4
phage P. putida Sci Big MOI 0.1 N.D. -0.7 N.D. -0.7 +0.3
carbon source carbon source 2-Deoxy-D-glucose 100 mM N.D. +0.1 N.D. -1.3 +0.2
carbon source carbon source D-Glucose 55.5 mM N.D. -0.2 N.D. -1.0 +0.1
pH D-Glucose (C) pH 6 N.D. -0.7 N.D. -0.4 +0.3
nitrogen source 2,6-diaminopimelic (N) N.D. +0.2 N.D. +0.2 -1.0
pH Growth at pH7 and (C) Trisodium citrate N.D. +0.3 N.D. -0.3 -0.7
phage P. putida PUT 7 MOI 1 N.D. +0.4 N.D. -0.2 -0.7
carbon source carbon source 3-O-methyl-glucose 10 mM N.D. +0.4 N.D. -1.1 +0.2
carbon source Levulinic Acid (C) N.D. +0.5 N.D. -0.8 -0.3
carbon source carbon source 4-vinylphenol 0.01 mM N.D. +0.3 N.D. -1.3 +0.6
temperature shift Temperature shift 30_to_21; with MOPS N.D. -0.7 N.D. +0.4 -0.2
pH pH 7 N.D. +0.1 N.D. -0.8 +0.3
nitrogen source D-2-Aminobutyric (N) N.D. -1.1 N.D. +0.8 -0.0
pH pH 5 N.D. +0.4 N.D. -0.9 +0.2
pH Growth at pH6 and (C) D-Glucose N.D. -0.9 N.D. +0.2 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. +0.0 N.D. +0.5 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.1 N.D. -0.8 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.2 N.D. -0.8 +0.9
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 N.D. -0.6 +0.7
pH pH 8 N.D. +0.5 N.D. -0.8 +0.3
carbon source carbon source Glycerol 108.6 mM N.D. +0.5 N.D. -0.9 +0.6
phage P. put South MOI 1 N.D. +0.8 N.D. -0.3 -0.2
pH pH 4 N.D. +0.5 N.D. -0.4 +0.3
pH pH 9 N.D. +0.3 N.D. -0.8 +0.9
phage PUT8 MOI 0.1 N.D. +0.6 N.D. +0.0 +0.7
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