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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1170 and PP_1171 are separated by 22 nucleotides
PP_1171 and PP_1173 are separated by 403 nucleotides
PP_1173 and PP_1174 are separated by 753 nucleotides
PP_1174 and PP_1176 are separated by 976 nucleotides
PP_1170: PP_1170 - D-glucaro-1,5-lactonase UxuL
(from data)
, at 1,345,182 to 1,346,063
_1170
PP_1171: PP_1171 - Uronate dehydrogenase (EC 1.1.1.203)
(from data)
, at 1,346,086 to 1,346,892
_1171
PP_1173: PP_1173 - porin-like protein, at 1,347,296 to 1,348,534
_1173
PP_1174: PP_1174 - conserved protein of unknown function, at 1,349,288 to 1,349,422
_1174
PP_1176: PP_1176 - conserved protein of unknown function, at 1,350,399 to 1,350,533
_1176
Group
Condition
PP
_1170
PP
_1171
PP
_1173
PP
_1174
PP
_1176
carbon source
D-Galacturonic Acid (C)
-6.1
-6.1
-0.3
+0.2
-1.5
carbon source
D-Galacturonic Acid (C)
-5.0
-5.9
-0.3
+0.1
-0.4
carbon source
D-Glucuronic gamma lactone (C)
-4.9
-5.6
+0.2
-0.2
+0.0
carbon source
D-Glucuronic gamma lactone (C)
-4.5
-6.0
+0.2
-0.2
+0.4
carbon source
Rhamnogalacturonan - from potato (C)
-2.2
-1.6
-0.3
+0.0
-0.9
carbon source
D-Glucose (C); with MOPS
+0.0
-0.1
-0.0
-0.4
-4.3
carbon source
D-Glucuronic Acid (C)
-2.5
-2.7
+0.4
+0.4
-0.2
carbon source
Rhamnogalacturonan - from potato (C)
-1.6
-1.7
-0.2
-0.0
-0.8
temperature shift
Temperature shift 30_to_21; with MOPS
-0.3
+0.2
-0.1
-0.0
-4.0
phage
P. putida Missley MOI 0.1
+0.2
+0.3
+0.1
+0.5
-5.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
-0.2
+0.1
+0.0
-3.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
+0.2
+0.2
-0.0
-0.1
-3.7
carbon source
D-Glucose (C)
-0.0
-0.2
-0.2
+0.2
-2.9
nitrogen source
no extra (N); with MOPS
-0.6
-0.1
+0.1
-0.5
-1.9
carbon source
carbon source D-Glucose 55.5 mM
-0.7
-0.1
-0.4
-0.5
-0.9
carbon source
carbon source Glycerol 108.6 mM
-0.5
-0.2
+0.0
-0.4
-1.6
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
-0.2
+0.0
-0.2
-0.5
-1.7
microaerobic m9
microaerobic M9
-0.4
-0.1
+0.2
+0.1
-2.4
temperature shift
Temperature shift 10_to_30
+0.3
-0.3
-0.1
-0.1
-2.3
phage
PUT8 MOI 0.1
+0.2
+0.1
+0.1
+0.0
-3.0
temperature
Growth at 25C
+0.2
-0.2
-0.1
-0.8
-1.6
pH
Growth at pH6 and (C) D-Glucose
+0.2
-0.1
+0.1
+0.1
-2.7
r2a control
R2A control
+0.1
+0.0
-0.2
+0.1
-2.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.4
-0.0
+0.3
+0.0
-2.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=72_hrs
-0.1
+0.1
+0.1
+0.4
-2.2
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.3
+0.2
+0.3
+0.1
-1.9
nitrogen source
Urea (N)
-0.0
-0.3
+0.4
+0.2
-1.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.4
+0.5
-0.6
-0.0
-1.7
carbon source
carbon source 4-vinylphenol 0.01 mM
-0.7
-0.4
-0.3
-0.2
+1.1
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.4
+0.1
+0.6
-1.7
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