Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1143 and PP_1144 overlap by 101 nucleotidesPP_1144 and PP_1145 are separated by 277 nucleotidesPP_1145 and PP_1146 are separated by 150 nucleotidesPP_1146 and PP_1147 are separated by 63 nucleotides PP_1143: PP_1143 - 3-hydroxyisobutyrate dehydrogenase family protein, at 1,308,481 to 1,309,368 _1143 PP_1144: PP_1144 - conserved membrane protein of unknown function, at 1,309,268 to 1,311,532 _1144 PP_1145: PP_1145 - RNA polymerase-associated protein RapA, at 1,311,810 to 1,314,656 _1145 PP_1146: PP_1146 - conserved exported protein of unknown function, at 1,314,807 to 1,315,502 _1146 PP_1147: PP_1147 - conserved protein of unknown function, at 1,315,566 to 1,315,895 _1147
Group Condition PP_1143 PP_1144 PP_1145 PP_1146 PP_1147
temperature shift Temperature shift 30_to_25; with MOPS -0.9 -0.5 -0.1 -0.2 -0.1
nitrogen source nitrate (N) -0.4 -0.2 -0.3 -0.3 -0.5
carbon source Ferulic Acid (C) -0.3 -0.1 -0.3 -0.1 -1.0
carbon source 4-Hydroxyvalerate (C) (40mM) +0.1 +0.0 -0.8 -0.0 -0.9
pH Growth at pH7 and (C) D-Glucose -1.4 +0.0 -0.2 -0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.0 -0.2 +0.2 -1.3
carbon source 5-Aminovaleric 10 mM (C) +0.3 +0.1 -0.9 +0.1 -0.8
carbon source ferulate (C); with MOPS +0.6 -0.2 -0.2 -0.5 -0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.4 -0.1 +0.1 -0.7
carbon source L-Lysine (C) -0.4 +0.2 -0.2 +0.2 -0.8
phage P. put South MOI 10 -0.5 -0.0 -0.6 +0.4 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.5 -0.4 -0.5 +0.0 -0.6
phage P. putida South MOI 10 -0.3 +0.2 -0.8 +0.3 -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 -0.1 -0.7 -0.2 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 +0.1 -0.5 +0.2 -0.7
carbon source ferulate (C); with MOPS -0.4 -0.2 +0.3 -0.6 +0.4
pH Growth at pH8 and (C) D-Glucose -0.4 -0.3 +0.1 -0.5 +0.5
nitrogen source D-2-Aminobutyric (N) -0.4 +0.1 +0.3 +0.4 -0.7
nitrogen source NAG (N); with MOPS +0.3 -0.1 -0.3 -1.1 +0.8
reactor 48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO. -0.3 -0.1 +0.8 -0.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.5 +0.1 +0.1 +0.3 -1.0
nitrogen source NAG (N); with MOPS +0.5 +0.3 -0.5 -0.2 +0.6
nitrogen source Propandiamine (N) -0.5 +0.0 +1.0 -0.0 +0.4
pH pH 7 -0.1 +0.4 +0.4 -0.5 +0.7
phage PUT 8 MOI 1 +0.1 +0.2 -0.4 +0.3 +1.1
phage P. putida PUT 7 MOI 1 +0.6 +0.2 +0.7 +0.6 -0.7
nitrogen source 2,6-diaminopimelic (N) +0.8 +0.3 +0.1 +0.4 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.1 +0.2 -0.1 +0.8
phage P. putida Missley MOI 0.1 -0.1 +0.4 +0.6 +0.3 +0.5
phage P. putida Sci small MOI 0.1 +1.0 +0.3 +0.5 +0.5 +0.4
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