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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1143 and PP_1144 overlap by 101 nucleotides
PP_1144 and PP_1145 are separated by 277 nucleotides
PP_1145 and PP_1146 are separated by 150 nucleotides
PP_1146 and PP_1147 are separated by 63 nucleotides
PP_1143: PP_1143 - 3-hydroxyisobutyrate dehydrogenase family protein, at 1,308,481 to 1,309,368
_1143
PP_1144: PP_1144 - conserved membrane protein of unknown function, at 1,309,268 to 1,311,532
_1144
PP_1145: PP_1145 - RNA polymerase-associated protein RapA, at 1,311,810 to 1,314,656
_1145
PP_1146: PP_1146 - conserved exported protein of unknown function, at 1,314,807 to 1,315,502
_1146
PP_1147: PP_1147 - conserved protein of unknown function, at 1,315,566 to 1,315,895
_1147
Group
Condition
PP
_1143
PP
_1144
PP
_1145
PP
_1146
PP
_1147
temperature shift
Temperature shift 30_to_25; with MOPS
-0.9
-0.5
-0.1
-0.2
-0.1
nitrogen source
nitrate (N)
-0.4
-0.2
-0.3
-0.3
-0.5
carbon source
Ferulic Acid (C)
-0.3
-0.1
-0.3
-0.1
-1.0
carbon source
4-Hydroxyvalerate (C) (40mM)
+0.1
+0.0
-0.8
-0.0
-0.9
pH
Growth at pH7 and (C) D-Glucose
-1.4
+0.0
-0.2
-0.1
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
-0.0
-0.2
+0.2
-1.3
carbon source
5-Aminovaleric 10 mM (C)
+0.3
+0.1
-0.9
+0.1
-0.8
carbon source
ferulate (C); with MOPS
+0.6
-0.2
-0.2
-0.5
-0.8
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
+0.4
-0.1
+0.1
-0.7
carbon source
L-Lysine (C)
-0.4
+0.2
-0.2
+0.2
-0.8
phage
P. put South MOI 10
-0.5
-0.0
-0.6
+0.4
-0.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.5
-0.4
-0.5
+0.0
-0.6
phage
P. putida South MOI 10
-0.3
+0.2
-0.8
+0.3
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.3
-0.1
-0.7
-0.2
+0.4
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
+0.3
+0.1
-0.5
+0.2
-0.7
carbon source
ferulate (C); with MOPS
-0.4
-0.2
+0.3
-0.6
+0.4
pH
Growth at pH8 and (C) D-Glucose
-0.4
-0.3
+0.1
-0.5
+0.5
nitrogen source
D-2-Aminobutyric (N)
-0.4
+0.1
+0.3
+0.4
-0.7
nitrogen source
NAG (N); with MOPS
+0.3
-0.1
-0.3
-1.1
+0.8
reactor
48hr timepoint of 1L M9/1% dextrose DISCONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat set to 10% DO.
-0.3
-0.1
+0.8
-0.1
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.5
+0.1
+0.1
+0.3
-1.0
nitrogen source
NAG (N); with MOPS
+0.5
+0.3
-0.5
-0.2
+0.6
nitrogen source
Propandiamine (N)
-0.5
+0.0
+1.0
-0.0
+0.4
pH
pH 7
-0.1
+0.4
+0.4
-0.5
+0.7
phage
PUT 8 MOI 1
+0.1
+0.2
-0.4
+0.3
+1.1
phage
P. putida PUT 7 MOI 1
+0.6
+0.2
+0.7
+0.6
-0.7
nitrogen source
2,6-diaminopimelic (N)
+0.8
+0.3
+0.1
+0.4
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.1
+0.2
-0.1
+0.8
phage
P. putida Missley MOI 0.1
-0.1
+0.4
+0.6
+0.3
+0.5
phage
P. putida Sci small MOI 0.1
+1.0
+0.3
+0.5
+0.5
+0.4
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