Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1120 and PP_1121 are separated by 159 nucleotidesPP_1121 and PP_1122 are separated by 14 nucleotidesPP_1122 and PP_1123 are separated by 98 nucleotidesPP_1123 and PP_1124 are separated by 116 nucleotides PP_1120: PP_1120 - conserved protein of unknown function, at 1,283,409 to 1,283,903 _1120 PP_1121: PP_1121 - OmpA family protein, at 1,284,063 to 1,284,755 _1121 PP_1122: PP_1122 - OmpA family protein, at 1,284,770 to 1,285,564 _1122 PP_1123: PP_1123 - conserved protein of unknown function, at 1,285,663 to 1,285,938 _1123 PP_1124: PP_1124 - conserved membrane protein of unknown function, at 1,286,055 to 1,286,519 _1124
Group Condition PP_1120 PP_1121 PP_1122 PP_1123 PP_1124
nitrogen source nitrogen source nitrite 1mM +0.4 +0.0 -4.5 N.D. +0.4
phage P. putida South MOI 10 +0.1 -0.2 -2.5 N.D. -0.4
nitrogen source 2,6-diaminopimelic (N) -0.2 -0.0 -1.6 N.D. -1.0
nitrogen source Propandiamine (N) -0.1 +0.2 -0.5 N.D. -2.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 -0.1 -2.1 N.D. +0.4
phage PUT 8 MOI 1 +0.1 -0.1 -2.5 N.D. +0.5
pH Growth at pH8 and (C) D-Glucose +0.1 +0.3 -2.2 N.D. -0.1
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL -0.1 +0.1 -1.9 N.D. +0.2
pH Valerolactone (C) pH 6.5 +0.0 +0.2 -0.7 N.D. -1.4
temperature Growth at 25C +0.0 -0.1 -2.6 N.D. +1.0
pH Growth at pH6 and (C) D-Glucose -0.5 +0.4 -0.3 N.D. -1.1
carbon source 3-methyl-1-butanol 0.25 vol% (C) +0.0 +0.0 +0.5 N.D. -1.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.2 +0.2 -1.6 N.D. +0.4
carbon source carbon source 3-O-methyl-glucose 10 mM -0.0 -0.1 -1.3 N.D. +0.9
stress Growth with Chloride 500 mM +0.1 +0.2 +1.0 N.D. -1.4
carbon source carbon source 2-Deoxy-D-glucose 10 mM +0.2 +0.5 -1.4 N.D. +0.9
pH Growth at pH7 and (C) D-Glucose -0.0 -1.0 +1.3 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 +0.4 -1.5 N.D. +0.9
pH pH 7 +0.2 +0.4 -1.5 N.D. +1.3
carbon source D-Glucose (C) -0.2 -0.4 +1.4 N.D. -0.3
pH Growth at pH8 and (C) Trisodium citrate +0.1 +0.4 +1.4 N.D. -1.2
carbon source ferulate (C); with MOPS +0.4 +0.5 -1.1 N.D. +1.0
phage P. putida Sci small MOI 0.1 +0.2 +0.5 -1.2 N.D. +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.1 -0.2 +1.6 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +0.6 -0.8 N.D. +2.1
pH pH 9 +0.2 -0.3 +0.4 N.D. +1.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.1 +1.2 N.D. +0.9
phage P. putida PUT 7 MOI 1 +0.4 +0.7 -0.2 N.D. +1.1
carbon source carbon source 4-vinylphenol 0.1 mM +0.2 +0.6 +0.7 N.D. +1.1
phage P. putida Missley MOI 0.1 +0.6 +1.1 +0.2 N.D. +0.9
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