Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1108 and PP_1109 are separated by 103 nucleotidesPP_1109 and PP_1110 are separated by 181 nucleotidesPP_1110 and PP_1111 overlap by 4 nucleotidesPP_1111 and PP_1112 overlap by 4 nucleotides PP_1108: PP_1108 - Acyl-homoserine lactone acylase QuiP, at 1,265,546 to 1,267,987 _1108 PP_1109: PP_1109 - Transcriptional regulator, GntR family, at 1,268,091 to 1,269,431 _1109 PP_1110: PP_1110 - serine acetyltransferase, at 1,269,613 to 1,270,317 _1110 PP_1111: PP_1111 - putative Synthetase, at 1,270,314 to 1,271,102 _1111 PP_1112: PP_1112 - conserved membrane protein of unknown function, at 1,271,099 to 1,272,310 _1112
Group Condition PP_1108 PP_1109 PP_1110 PP_1111 PP_1112
carbon source D-Glucaric (C) +0.3 -4.7 -8.1 -3.2 -0.3
carbon source carbon source D-Glucose 55.5 mM -0.1 -4.9 -6.3 -4.8 +0.5
carbon source Lauric (C) +0.1 -5.0 -3.9 -5.0 -1.6
pH pH 4 -0.1 -5.9 -5.7 -4.5 +0.9
carbon source carbon source Bisabolene 48.9 mM -0.0 -5.1 -5.9 -4.7 +0.5
carbon source carbon source 2-Deoxy-D-glucose 10 mM -0.2 -4.5 -6.1 -4.2 +0.5
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -0.2 -5.2 -4.9 -3.8 -0.4
carbon source Mucic (C) -0.0 -5.4 -4.4 -3.7 -0.9
carbon source Mucic (C) +0.1 -5.3 -4.1 -3.7 -1.2
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. +0.0 -4.9 -5.2 -4.4 +0.4
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -0.2 -5.0 -5.1 -3.7 -0.3
carbon source carbon source 2-Deoxy-D-glucose 100 mM -0.2 -4.5 -5.9 -4.0 +0.6
carbon source rac-3-Hydroxypentanoic Acid (C) +0.2 -4.4 -5.9 -3.8 -0.1
carbon source Lauric (C) +0.1 -4.4 -3.9 -4.4 -1.3
carbon source carbon source Glycerol 108.6 mM +0.2 -4.6 -5.1 -4.8 +0.6
carbon source carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM +0.1 -5.1 -4.8 -4.5 +0.5
pH pH 8 -0.2 -4.6 -5.7 -4.0 +0.9
pH pH 9 -0.3 -4.7 -4.5 -4.8 +0.8
carbon source carbon source Bisabolol 45 mM -0.1 -4.9 -4.9 -4.1 +0.6
carbon source carbon source 2-Deoxy-D-glucose 50 mM -0.2 -4.5 -4.7 -4.2 +0.6
carbon source carbon source 3-O-methyl-glucose 50 mM -0.4 -4.6 -4.8 -4.0 +0.9
pH pH 5 +0.1 -4.8 -5.1 -4.3 +1.3
carbon source 1,3-Hexanediol (C) -0.0 -3.7 -5.9 -3.4 +0.2
carbon source carbon source 4-vinylphenol 0.01 mM -0.1 -5.2 -4.9 -3.3 +0.8
carbon source Decanoic (C) +0.1 -5.0 -3.3 -4.0 -0.4
reactor 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. -0.1 -4.6 -4.8 -3.3 +0.4
reactor 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. +0.1 -5.0 -3.9 -4.0 +0.5
carbon source Decanoic (C) +0.2 -5.3 -2.3 -4.4 -0.6
carbon source carbon source 4-vinylphenol 0.1 mM -0.1 -4.2 -5.3 -3.8 +1.5
pH pH 7 -0.4 -4.4 -3.9 -4.1 +1.1
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