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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1108 and PP_1109 are separated by 103 nucleotides
PP_1109 and PP_1110 are separated by 181 nucleotides
PP_1110 and PP_1111 overlap by 4 nucleotides
PP_1111 and PP_1112 overlap by 4 nucleotides
PP_1108: PP_1108 - Acyl-homoserine lactone acylase QuiP, at 1,265,546 to 1,267,987
_1108
PP_1109: PP_1109 - Transcriptional regulator, GntR family, at 1,268,091 to 1,269,431
_1109
PP_1110: PP_1110 - serine acetyltransferase, at 1,269,613 to 1,270,317
_1110
PP_1111: PP_1111 - putative Synthetase, at 1,270,314 to 1,271,102
_1111
PP_1112: PP_1112 - conserved membrane protein of unknown function, at 1,271,099 to 1,272,310
_1112
Group
Condition
PP
_1108
PP
_1109
PP
_1110
PP
_1111
PP
_1112
carbon source
D-Glucaric (C)
+0.3
-4.7
-8.1
-3.2
-0.3
carbon source
carbon source D-Glucose 55.5 mM
-0.1
-4.9
-6.3
-4.8
+0.5
carbon source
Lauric (C)
+0.1
-5.0
-3.9
-5.0
-1.6
pH
pH 4
-0.1
-5.9
-5.7
-4.5
+0.9
carbon source
carbon source Bisabolene 48.9 mM
-0.0
-5.1
-5.9
-4.7
+0.5
carbon source
carbon source 2-Deoxy-D-glucose 10 mM
-0.2
-4.5
-6.1
-4.2
+0.5
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-0.2
-5.2
-4.9
-3.8
-0.4
carbon source
Mucic (C)
-0.0
-5.4
-4.4
-3.7
-0.9
carbon source
Mucic (C)
+0.1
-5.3
-4.1
-3.7
-1.2
reactor
40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
+0.0
-4.9
-5.2
-4.4
+0.4
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-0.2
-5.0
-5.1
-3.7
-0.3
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
-0.2
-4.5
-5.9
-4.0
+0.6
carbon source
rac-3-Hydroxypentanoic Acid (C)
+0.2
-4.4
-5.9
-3.8
-0.1
carbon source
Lauric (C)
+0.1
-4.4
-3.9
-4.4
-1.3
carbon source
carbon source Glycerol 108.6 mM
+0.2
-4.6
-5.1
-4.8
+0.6
carbon source
carbon source D-Xylose 66.6 mM and Glycerol 108.6 mM
+0.1
-5.1
-4.8
-4.5
+0.5
pH
pH 8
-0.2
-4.6
-5.7
-4.0
+0.9
pH
pH 9
-0.3
-4.7
-4.5
-4.8
+0.8
carbon source
carbon source Bisabolol 45 mM
-0.1
-4.9
-4.9
-4.1
+0.6
carbon source
carbon source 2-Deoxy-D-glucose 50 mM
-0.2
-4.5
-4.7
-4.2
+0.6
carbon source
carbon source 3-O-methyl-glucose 50 mM
-0.4
-4.6
-4.8
-4.0
+0.9
pH
pH 5
+0.1
-4.8
-5.1
-4.3
+1.3
carbon source
1,3-Hexanediol (C)
-0.0
-3.7
-5.9
-3.4
+0.2
carbon source
carbon source 4-vinylphenol 0.01 mM
-0.1
-5.2
-4.9
-3.3
+0.8
carbon source
Decanoic (C)
+0.1
-5.0
-3.3
-4.0
-0.4
reactor
24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
-0.1
-4.6
-4.8
-3.3
+0.4
reactor
40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
+0.1
-5.0
-3.9
-4.0
+0.5
carbon source
Decanoic (C)
+0.2
-5.3
-2.3
-4.4
-0.6
carbon source
carbon source 4-vinylphenol 0.1 mM
-0.1
-4.2
-5.3
-3.8
+1.5
pH
pH 7
-0.4
-4.4
-3.9
-4.1
+1.1
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