Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1050 and PP_1051 overlap by 43 nucleotidesPP_1051 and PP_1052 overlap by 4 nucleotidesPP_1052 and PP_1053 overlap by 16 nucleotidesPP_1053 and PP_1054 overlap by 4 nucleotides PP_1050: PP_1050 - Type II secretion pathway protein XcpU, at 1,200,804 to 1,201,235 _1050 PP_1051: PP_1051 - Type II secretion pathway protein XcpV, at 1,201,193 to 1,201,600 _1051 PP_1052: PP_1052 - Type II secretion pathway protein XcpW, at 1,201,597 to 1,202,187 _1052 PP_1053: PP_1053 - Type II secretion pathway protein XcpY, at 1,202,172 to 1,203,260 _1053 PP_1054: PP_1054 - Type II secretion pathway protein XcpZ, at 1,203,257 to 1,203,643 _1054
Group Condition PP_1050 PP_1051 PP_1052 PP_1053 PP_1054
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -1.5 N.D. -0.7 -3.9
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.1 N.D. -1.3 -3.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs N.D. +0.2 N.D. -0.3 -4.5
phage P. putida Missley MOI 0.1 N.D. -0.6 N.D. -0.6 -3.2
carbon source D-Glucaric (C) N.D. -0.5 N.D. -0.0 -3.8
pH D-Glucose (C) pH 6 N.D. -0.2 N.D. -0.7 -3.0
nitrogen source D-2-Aminobutyric (N) N.D. -0.3 N.D. -0.4 -2.9
pH pH 9 N.D. -0.8 N.D. +0.4 -2.7
stress MOPS minimal media_Glucose20mM with PHB-HV-HH 0.1 mg/mL N.D. -0.1 N.D. -0.3 -2.6
carbon source Mucic (C) N.D. +0.0 N.D. -0.0 -3.0
stress MOPS minimal media_Glucose20mM with Carbenicillin 100 .g/mL N.D. +0.1 N.D. +0.0 -3.0
carbon source Tween 20 (C) N.D. -0.6 N.D. -0.5 -1.7
carbon source carbon source 2-Deoxy-D-glucose 10 mM N.D. -1.4 N.D. -0.0 -1.3
stress MOPS minimal media_Glucose20mM with Chloramphenicol 25 .g/mL N.D. -0.2 N.D. +0.4 -2.9
carbon source D-Glucose (C) with 20AA_mix_minus_Cys N.D. +0.3 N.D. -0.3 -2.6
temperature shift Temperature shift 30_to_25; with MOPS N.D. +0.0 N.D. -0.0 -2.6
pH Growth at pH6 and (C) D-Glucose N.D. -0.2 N.D. -0.5 -2.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs N.D. -0.9 N.D. -0.2 -1.6
carbon source butyrate (C) N.D. +0.1 N.D. -0.1 -2.3
stress Growth with Chloride 500 mM; with MOPS N.D. +0.3 N.D. -0.5 -2.1
carbon source carbon source 4-vinylphenol 0.1 mM N.D. -0.1 N.D. +0.2 -2.4
carbon source gluconate (C) N.D. -0.6 N.D. +0.3 -1.6
nitrogen source nitrite (N) N.D. +0.5 N.D. +0.1 -2.2
stress MOPS minimal media_Glucose20mM with Chloride 200 mM N.D. +0.5 N.D. +0.2 -1.9
pH Growth at pH6 and (C) Trisodium citrate N.D. +0.7 N.D. -0.9 +1.1
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay N.D. -0.7 N.D. -0.0 +1.9
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay N.D. -1.0 N.D. +0.0 +2.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.2 N.D. -0.5 +0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.0 N.D. -0.3 +2.1
nitrogen source D-2-Aminobutyric (N) N.D. +1.4 N.D. +0.4 +2.4
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