Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1008 and PP_1009 are separated by 256 nucleotidesPP_1009 and PP_1010 are separated by 264 nucleotidesPP_1010 and PP_1011 overlap by 4 nucleotidesPP_1011 and PP_1012 are separated by 78 nucleotides PP_1008: PP_1008 - RNA polymerase subunit sigma-24, at 1,149,241 to 1,149,753 _1008 PP_1009: PP_1009 - glyceraldehyde-3-phosphate dehydrogenase, at 1,150,010 to 1,151,011 _1009 PP_1010: PP_1010 - phosphogluconate dehydratase, at 1,151,276 to 1,153,102 _1010 PP_1011: PP_1011 - glucokinase, at 1,153,099 to 1,154,058 _1011 PP_1012: PP_1012 - DNA-binding response regulator, at 1,154,137 to 1,154,862 _1012
Group Condition PP_1008 PP_1009 PP_1010 PP_1011 PP_1012
nitrogen source D-2-Aminobutyric (N) -0.9 -1.4 N.D. -3.4 -3.6
pH pH 4 +1.4 -1.7 N.D. -3.9 -4.6
pH pH 5 +1.0 -1.2 N.D. -3.7 -4.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -0.4 N.D. -3.5 -4.5
nitrogen source D-2-Aminobutyric (N) +0.3 -2.0 N.D. -2.7 -3.8
nitrogen source Propandiamine (N) +0.2 +0.3 N.D. -3.8 -4.4
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM +0.0 -1.4 N.D. -3.1 -2.4
nitrogen source Propandiamine (N) +0.5 -0.8 N.D. -3.1 -3.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 -0.3 N.D. -2.6 -3.9
pH pH 8 +0.9 -1.5 N.D. -3.1 -2.3
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM +0.7 -1.3 N.D. -3.0 -2.1
pH pH 6 -0.4 -1.1 N.D. -2.7 -1.3
pH pH 7 -0.4 -0.3 N.D. -2.3 -2.3
temperature shift Temperature shift 30_to_25; with MOPS +0.2 -2.8 N.D. -1.2 -1.4
phage P. putida Sci Big MOI 0.1 -4.6 +0.2 N.D. -0.3 -0.2
reactor 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -0.2 -1.0 N.D. -1.8 -1.8
nitrogen source Urea (N) -1.4 -1.3 N.D. -1.0 -1.2
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO. -1.0 -0.7 N.D. -1.7 -1.4
nitrogen source Urea (N) -0.6 -0.8 N.D. -1.8 -1.6
nitrogen source nitrogen source nitrite 1mM -1.8 -0.4 N.D. -1.5 -1.0
pH pH 9 +0.5 -0.7 N.D. -2.6 -1.9
reactor 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.8 -0.7 N.D. -1.6 -1.4
pH Growth at pH9 and (C) D-Glucose -0.0 -0.1 N.D. -2.9 -1.4
nitrogen source nitrogen source Ammonium chloride 20mM +0.1 -0.8 N.D. -2.3 -1.3
carbon source carbon source D-Glucose 55.5 mM -0.6 -2.0 N.D. -1.5 -0.3
pH Growth at pH9 and (C) D-Glucose +0.2 -0.3 N.D. -2.3 -1.7
carbon source carbon source 2-Deoxy-D-glucose 100 mM +0.8 -1.1 N.D. -1.9 -1.7
nitrogen source nitrite (N) +0.3 -0.8 N.D. -2.4 -0.8
temperature shift Temperature shift 30_to_25 +0.9 -1.1 N.D. -1.8 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.2 +0.9 N.D. -0.5 -0.7
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