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Protein
Homologs
Fitness for 5 genes in
Pseudomonas putida KT2440
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500 nt
PP_1008 and PP_1009 are separated by 256 nucleotides
PP_1009 and PP_1010 are separated by 264 nucleotides
PP_1010 and PP_1011 overlap by 4 nucleotides
PP_1011 and PP_1012 are separated by 78 nucleotides
PP_1008: PP_1008 - RNA polymerase subunit sigma-24, at 1,149,241 to 1,149,753
_1008
PP_1009: PP_1009 - glyceraldehyde-3-phosphate dehydrogenase, at 1,150,010 to 1,151,011
_1009
PP_1010: PP_1010 - phosphogluconate dehydratase, at 1,151,276 to 1,153,102
_1010
PP_1011: PP_1011 - glucokinase, at 1,153,099 to 1,154,058
_1011
PP_1012: PP_1012 - DNA-binding response regulator, at 1,154,137 to 1,154,862
_1012
Group
Condition
PP
_1008
PP
_1009
PP
_1010
PP
_1011
PP
_1012
nitrogen source
D-2-Aminobutyric (N)
-0.9
-1.4
N.D.
-3.4
-3.6
pH
pH 4
+1.4
-1.7
N.D.
-3.9
-4.6
pH
pH 5
+1.0
-1.2
N.D.
-3.7
-4.6
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.0
-0.4
N.D.
-3.5
-4.5
nitrogen source
D-2-Aminobutyric (N)
+0.3
-2.0
N.D.
-2.7
-3.8
nitrogen source
Propandiamine (N)
+0.2
+0.3
N.D.
-3.8
-4.4
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
+0.0
-1.4
N.D.
-3.1
-2.4
nitrogen source
Propandiamine (N)
+0.5
-0.8
N.D.
-3.1
-3.4
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.0
-0.3
N.D.
-2.6
-3.9
pH
pH 8
+0.9
-1.5
N.D.
-3.1
-2.3
stress
MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM
+0.7
-1.3
N.D.
-3.0
-2.1
pH
pH 6
-0.4
-1.1
N.D.
-2.7
-1.3
pH
pH 7
-0.4
-0.3
N.D.
-2.3
-2.3
temperature shift
Temperature shift 30_to_25; with MOPS
+0.2
-2.8
N.D.
-1.2
-1.4
phage
P. putida Sci Big MOI 0.1
-4.6
+0.2
N.D.
-0.3
-0.2
reactor
24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-0.2
-1.0
N.D.
-1.8
-1.8
nitrogen source
Urea (N)
-1.4
-1.3
N.D.
-1.0
-1.2
reactor
72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO.
-1.0
-0.7
N.D.
-1.7
-1.4
nitrogen source
Urea (N)
-0.6
-0.8
N.D.
-1.8
-1.6
nitrogen source
nitrogen source nitrite 1mM
-1.8
-0.4
N.D.
-1.5
-1.0
pH
pH 9
+0.5
-0.7
N.D.
-2.6
-1.9
reactor
48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
-0.8
-0.7
N.D.
-1.6
-1.4
pH
Growth at pH9 and (C) D-Glucose
-0.0
-0.1
N.D.
-2.9
-1.4
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.1
-0.8
N.D.
-2.3
-1.3
carbon source
carbon source D-Glucose 55.5 mM
-0.6
-2.0
N.D.
-1.5
-0.3
pH
Growth at pH9 and (C) D-Glucose
+0.2
-0.3
N.D.
-2.3
-1.7
carbon source
carbon source 2-Deoxy-D-glucose 100 mM
+0.8
-1.1
N.D.
-1.9
-1.7
nitrogen source
nitrite (N)
+0.3
-0.8
N.D.
-2.4
-0.8
temperature shift
Temperature shift 30_to_25
+0.9
-1.1
N.D.
-1.8
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+2.2
+0.9
N.D.
-0.5
-0.7
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