Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_1006 and PP_1007 are separated by 126 nucleotidesPP_1007 and PP_1008 overlap by 4 nucleotidesPP_1008 and PP_1009 are separated by 256 nucleotidesPP_1009 and PP_1010 are separated by 264 nucleotides PP_1006: PP_1006 - putative outer membrane heme receptor, at 1,145,474 to 1,148,062 _1006 PP_1007: PP_1007 - putative Transmembrane sensor, at 1,148,189 to 1,149,244 _1007 PP_1008: PP_1008 - RNA polymerase subunit sigma-24, at 1,149,241 to 1,149,753 _1008 PP_1009: PP_1009 - glyceraldehyde-3-phosphate dehydrogenase, at 1,150,010 to 1,151,011 _1009 PP_1010: PP_1010 - phosphogluconate dehydratase, at 1,151,276 to 1,153,102 _1010
Group Condition PP_1006 PP_1007 PP_1008 PP_1009 PP_1010
phage P. putida Sci Big MOI 0.1 -0.0 -0.2 -4.6 +0.2 N.D.
carbon source 3-methyl-1-butanol (C) -0.1 -1.4 -0.3 -2.8 N.D.
carbon source Myristic (C) +0.1 -0.3 -2.7 -0.1 N.D.
carbon source 3-methyl-1-butanol (C) -0.0 -0.7 -0.3 -1.8 N.D.
carbon source 3-methyl-1-butanol 0.1 vol% (C) -0.3 -0.7 +0.2 -2.1 N.D.
temperature shift Temperature shift 30_to_25; with MOPS -0.1 +0.3 +0.2 -2.8 N.D.
carbon source carbon source D-Glucose 55.5 mM +0.1 +0.1 -0.6 -2.0 N.D.
reactor 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. -0.0 +0.5 -2.3 -0.5 N.D.
pH Growth at pH6 and (C) Trisodium citrate +0.1 +0.4 -1.9 -0.7 N.D.
nitrogen source Urea (N) +0.1 +0.4 -1.4 -1.3 N.D.
carbon source D-Glucose (C) +0.0 +0.1 -0.7 -1.5 N.D.
carbon source D-Fructose (C) -0.1 +0.2 -0.3 -1.8 N.D.
nitrogen source D-2-Aminobutyric (N) -0.1 +0.3 -0.9 -1.4 N.D.
nitrogen source nitrogen source nitrite 1mM +0.1 +0.3 -1.8 -0.4 N.D.
carbon source carbon source 3-O-methyl-glucose 50 mM +0.1 +0.4 -1.0 -1.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X +0.2 -0.6 -1.4 +0.2 N.D.
nitrogen source D-2-Aminobutyric (N) -0.0 +0.2 +0.3 -2.0 N.D.
temperature shift Temperature shift 30_to_25; with MOPS -0.2 -0.0 +0.7 -1.4 N.D.
stress MOPS minimal media_Glucose20mM with Fluoroacetate 80 mM +0.1 -0.3 +0.7 -1.3 N.D.
pH pH 8 +0.2 -0.1 +0.9 -1.5 N.D.
pH Growth at pH6 and (C) Trisodium citrate +0.1 +0.6 -1.4 +0.4 N.D.
pH pH 4 -0.1 +0.1 +1.4 -1.7 N.D.
pH pH 5 +0.0 +0.3 +1.0 -1.2 N.D.
temperature shift Temperature shift 30_to_25 +0.1 +0.3 +0.9 -1.1 N.D.
pH Growth at pH7 and (C) D-Glucose -0.1 +0.6 +0.8 -0.9 N.D.
carbon source D-Glucose (C) +0.0 +0.1 +1.8 -1.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.8 -0.3 +0.6 +0.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +2.1 -0.9 +1.8 -0.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +2.4 -0.6 +2.2 -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.5 +2.2 +0.9 N.D.
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