Fitness for 5 genes in Pseudomonas putida KT2440

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500 ntPP_0995 and PP_0996 are separated by 77 nucleotidesPP_0996 and PP_0997 are separated by 127 nucleotidesPP_0997 and PP_0998 are separated by 140 nucleotidesPP_0998 and PP_0999 overlap by 4 nucleotides PP_0995: PP_0995 - conserved membrane protein of unknown function, at 1,133,414 to 1,134,055 _0995 PP_0996: PP_0996 - glutamate:sodium symporter, at 1,134,133 to 1,135,338 _0996 PP_0997: PP_0997 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 1,135,466 to 1,136,974 _0997 PP_0998: PP_0998 - conserved protein of unknown function, at 1,137,115 to 1,137,543 _0998 PP_0999: PP_0999 - carbamate kinase, at 1,137,540 to 1,138,469 _0999
Group Condition PP_0995 PP_0996 PP_0997 PP_0998 PP_0999
nitrogen source Hippuric (N) -1.9 +0.2 -5.7 +0.1 +0.1
nitrogen source Hippuric (N) -1.8 +0.1 -5.0 -0.2 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.2 -0.0 -2.0 -4.1
carbon source Delta-Nonalactone (C) -1.4 +0.1 -4.5 -0.1 -0.3
nitrogen source Glycine (N) -0.8 +0.2 -4.5 +0.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -2.0 -0.2 +0.4 -0.7 -2.6
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X -0.3 -0.0 -0.4 -1.7 -2.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.9 -0.1 -0.3 -0.6 -2.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.0 -0.1 -1.6 -2.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.0 +0.1 -1.8 -3.3
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X -0.2 -0.2 -0.1 -1.4 -2.8
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.2X -0.4 -0.2 +0.0 -1.4 -2.6
nitrogen source Glycine (N) -0.6 +0.0 -4.0 +0.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X -0.4 -0.2 -0.1 -1.6 -2.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.2 -0.2 -1.2 -2.7
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.1 -0.1 -0.1 -2.0 -2.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.0 -0.1 -0.0 -1.7 -2.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.1 -0.1 -1.2 -2.6
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X -0.3 -0.1 -0.1 -1.6 -1.9
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.4 +0.1 -0.0 -0.8 -1.8
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.2X -0.3 +0.0 -0.3 -1.6 -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 +0.4 -0.3 -1.7 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.4 -0.1 -0.0 +0.2 -2.3
carbon source Delta-Decalactone (C) -1.6 +0.2 -2.2 -0.1 +0.1
carbon source ferulate (C); with MOPS -2.3 -0.2 +0.1 +0.1 -1.4
r2a control R2A control -0.2 +0.0 +0.2 -1.6 -2.0
carbon source Delta-Decalactone (C) -1.5 -0.0 -2.3 -0.0 +0.6
nitrogen source nitrogen source nitrate 5mM +0.4 +0.3 -0.5 -1.6 -1.5
nitrogen source NAG (N); with MOPS +0.4 +0.3 +0.0 +0.1 -2.9
temperature Growth at 25C; with MOPS +0.6 +0.1 -0.3 -1.7 +1.1
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